Transport of environmental natural organic matter coated silver nanoparticle across cell membrane based on membrane etching treatment and inhibitors
Abstract Environmental natural organic matters (NOMs) have great effects on the physicochemical properties of engineering nanoparticles, which may impact the transport of nanoparticles across plasma membrane and the cytotoxicity. Therefore, the kinetics, uptake pathway and mass of transporting into...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Nature Publishing Group
2021-01-01
|
Series: | Scientific Reports |
Online Access: | https://doi.org/10.1038/s41598-020-79901-y |
id |
doaj-86f00ea4238f4885b92cbcd60fc34e00 |
---|---|
record_format |
Article |
spelling |
doaj-86f00ea4238f4885b92cbcd60fc34e002021-01-17T12:32:05ZengNature Publishing GroupScientific Reports2045-23222021-01-0111111310.1038/s41598-020-79901-yTransport of environmental natural organic matter coated silver nanoparticle across cell membrane based on membrane etching treatment and inhibitorsLaijin Zhong0Sisi Chen1Zhijie Tang2Xuewen Guo3Xin Hu4Weijuan Zheng5Hong-zhen Lian6State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry & Chemical Engineering and Centre of Materials Analysis, Nanjing UniversityState Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry & Chemical Engineering and Centre of Materials Analysis, Nanjing UniversityState Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry & Chemical Engineering and Centre of Materials Analysis, Nanjing UniversityState Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry & Chemical Engineering and Centre of Materials Analysis, Nanjing UniversityState Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry & Chemical Engineering and Centre of Materials Analysis, Nanjing UniversityState Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing UniversityState Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry & Chemical Engineering and Centre of Materials Analysis, Nanjing UniversityAbstract Environmental natural organic matters (NOMs) have great effects on the physicochemical properties of engineering nanoparticles, which may impact the transport of nanoparticles across plasma membrane and the cytotoxicity. Therefore, the kinetics, uptake pathway and mass of transporting into A549 cell membrane of silver nanoparticles (AgNPs) coated with citric acid (CA), tartaric acid (TA) and fulvic acid (FA) were investigated, respectively. CA, FA and TA enhanced the colloidal stability of AgNPs in culture medium and have greatly changed the surface plasmon resonance spectrum of AgNPs due to the absorption of CA, FA and TA on surface of AgNPs. Internalizing model showed that velocity of CA-, TA- and FA-nAg transporting into A549 cell were 5.82-, 1.69- and 0.29-fold higher than those of the control group, respectively. Intracellular mass of Ag was dependent on mass of AgNPs delivered to cell from suspension, which obeyed Logistic model and was affected by NOMs that CA- and TA-nAg showed a large promotion on intracellular mass of Ag. The lipid raft/caveolae-mediated endocytosis (LME) of A549 cell uptake of AgNPs were susceptible to CA, TA and FA that uptake of CA-, TA- and FA-nAg showed lower degree of dependent on LME than that of the control (uncoated AgNPs). Actin-involved uptake pathway and macropinocytosis would have less contribution to uptake of FA-nAg. Overall, transmembrane transport of NOMs-coated AgNPs differs greatly from that of the pristine AgNPs.https://doi.org/10.1038/s41598-020-79901-y |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Laijin Zhong Sisi Chen Zhijie Tang Xuewen Guo Xin Hu Weijuan Zheng Hong-zhen Lian |
spellingShingle |
Laijin Zhong Sisi Chen Zhijie Tang Xuewen Guo Xin Hu Weijuan Zheng Hong-zhen Lian Transport of environmental natural organic matter coated silver nanoparticle across cell membrane based on membrane etching treatment and inhibitors Scientific Reports |
author_facet |
Laijin Zhong Sisi Chen Zhijie Tang Xuewen Guo Xin Hu Weijuan Zheng Hong-zhen Lian |
author_sort |
Laijin Zhong |
title |
Transport of environmental natural organic matter coated silver nanoparticle across cell membrane based on membrane etching treatment and inhibitors |
title_short |
Transport of environmental natural organic matter coated silver nanoparticle across cell membrane based on membrane etching treatment and inhibitors |
title_full |
Transport of environmental natural organic matter coated silver nanoparticle across cell membrane based on membrane etching treatment and inhibitors |
title_fullStr |
Transport of environmental natural organic matter coated silver nanoparticle across cell membrane based on membrane etching treatment and inhibitors |
title_full_unstemmed |
Transport of environmental natural organic matter coated silver nanoparticle across cell membrane based on membrane etching treatment and inhibitors |
title_sort |
transport of environmental natural organic matter coated silver nanoparticle across cell membrane based on membrane etching treatment and inhibitors |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2021-01-01 |
description |
Abstract Environmental natural organic matters (NOMs) have great effects on the physicochemical properties of engineering nanoparticles, which may impact the transport of nanoparticles across plasma membrane and the cytotoxicity. Therefore, the kinetics, uptake pathway and mass of transporting into A549 cell membrane of silver nanoparticles (AgNPs) coated with citric acid (CA), tartaric acid (TA) and fulvic acid (FA) were investigated, respectively. CA, FA and TA enhanced the colloidal stability of AgNPs in culture medium and have greatly changed the surface plasmon resonance spectrum of AgNPs due to the absorption of CA, FA and TA on surface of AgNPs. Internalizing model showed that velocity of CA-, TA- and FA-nAg transporting into A549 cell were 5.82-, 1.69- and 0.29-fold higher than those of the control group, respectively. Intracellular mass of Ag was dependent on mass of AgNPs delivered to cell from suspension, which obeyed Logistic model and was affected by NOMs that CA- and TA-nAg showed a large promotion on intracellular mass of Ag. The lipid raft/caveolae-mediated endocytosis (LME) of A549 cell uptake of AgNPs were susceptible to CA, TA and FA that uptake of CA-, TA- and FA-nAg showed lower degree of dependent on LME than that of the control (uncoated AgNPs). Actin-involved uptake pathway and macropinocytosis would have less contribution to uptake of FA-nAg. Overall, transmembrane transport of NOMs-coated AgNPs differs greatly from that of the pristine AgNPs. |
url |
https://doi.org/10.1038/s41598-020-79901-y |
work_keys_str_mv |
AT laijinzhong transportofenvironmentalnaturalorganicmattercoatedsilvernanoparticleacrosscellmembranebasedonmembraneetchingtreatmentandinhibitors AT sisichen transportofenvironmentalnaturalorganicmattercoatedsilvernanoparticleacrosscellmembranebasedonmembraneetchingtreatmentandinhibitors AT zhijietang transportofenvironmentalnaturalorganicmattercoatedsilvernanoparticleacrosscellmembranebasedonmembraneetchingtreatmentandinhibitors AT xuewenguo transportofenvironmentalnaturalorganicmattercoatedsilvernanoparticleacrosscellmembranebasedonmembraneetchingtreatmentandinhibitors AT xinhu transportofenvironmentalnaturalorganicmattercoatedsilvernanoparticleacrosscellmembranebasedonmembraneetchingtreatmentandinhibitors AT weijuanzheng transportofenvironmentalnaturalorganicmattercoatedsilvernanoparticleacrosscellmembranebasedonmembraneetchingtreatmentandinhibitors AT hongzhenlian transportofenvironmentalnaturalorganicmattercoatedsilvernanoparticleacrosscellmembranebasedonmembraneetchingtreatmentandinhibitors |
_version_ |
1724334680715558912 |