Hookah Smoke Mediates Cancer-Associated Epigenomic and Transcriptomic Signatures in Human Respiratory Epithelial Cells
Introduction: Although communal smoking of hookah by means of water pipes is perceived to be a safe alternative to cigarette smoking, the effects of hookah smoke in respiratory epithelia have not been well characterized. This study evaluated epigenomic and transcriptomic effects of hookah smoke rela...
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Elsevier
2021-07-01
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Series: | JTO Clinical and Research Reports |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S2666364321000400 |
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doaj-873846f098014644911c9514ccac2c84 |
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Article |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yin Xiong, PhD Sichuan Xi, PhD Sudheer Kumar Gara, PhD Jigui Shan, PhD James Gao, PhD Mary Zhang, MS Vivek Shukla, PhD Ruihong Wang, PhD Chuong D. Hoang, MD Haobin Chen, MD, PhD David S. Schrump, MD, MBA |
spellingShingle |
Yin Xiong, PhD Sichuan Xi, PhD Sudheer Kumar Gara, PhD Jigui Shan, PhD James Gao, PhD Mary Zhang, MS Vivek Shukla, PhD Ruihong Wang, PhD Chuong D. Hoang, MD Haobin Chen, MD, PhD David S. Schrump, MD, MBA Hookah Smoke Mediates Cancer-Associated Epigenomic and Transcriptomic Signatures in Human Respiratory Epithelial Cells JTO Clinical and Research Reports Hookah smoke Cigarette smoke Respiratory epithelia EREG FILIP1L ABI3BP |
author_facet |
Yin Xiong, PhD Sichuan Xi, PhD Sudheer Kumar Gara, PhD Jigui Shan, PhD James Gao, PhD Mary Zhang, MS Vivek Shukla, PhD Ruihong Wang, PhD Chuong D. Hoang, MD Haobin Chen, MD, PhD David S. Schrump, MD, MBA |
author_sort |
Yin Xiong, PhD |
title |
Hookah Smoke Mediates Cancer-Associated Epigenomic and Transcriptomic Signatures in Human Respiratory Epithelial Cells |
title_short |
Hookah Smoke Mediates Cancer-Associated Epigenomic and Transcriptomic Signatures in Human Respiratory Epithelial Cells |
title_full |
Hookah Smoke Mediates Cancer-Associated Epigenomic and Transcriptomic Signatures in Human Respiratory Epithelial Cells |
title_fullStr |
Hookah Smoke Mediates Cancer-Associated Epigenomic and Transcriptomic Signatures in Human Respiratory Epithelial Cells |
title_full_unstemmed |
Hookah Smoke Mediates Cancer-Associated Epigenomic and Transcriptomic Signatures in Human Respiratory Epithelial Cells |
title_sort |
hookah smoke mediates cancer-associated epigenomic and transcriptomic signatures in human respiratory epithelial cells |
publisher |
Elsevier |
series |
JTO Clinical and Research Reports |
issn |
2666-3643 |
publishDate |
2021-07-01 |
description |
Introduction: Although communal smoking of hookah by means of water pipes is perceived to be a safe alternative to cigarette smoking, the effects of hookah smoke in respiratory epithelia have not been well characterized. This study evaluated epigenomic and transcriptomic effects of hookah smoke relative to cigarette smoke in human respiratory epithelial cells. Methods: Primary normal human small airway epithelial cells from three donors and cdk4 and hTERT-immortalized small airway epithelial cells and human bronchial epithelial cells were cultured for 5 days in normal media with or without cigarette smoke condensates (CSCs) or water pipe condensates (WPCs). Cell count, immunoblot, RNA sequencing, quantitative real-time reverse-transcriptase polymerase chain reaction, methylation-specific polymerase chain reaction, and quantitative chromatin immunoprecipitation techniques were used to compare effects of hookah and cigarette smoke on cell proliferation, global histone marks, gene expression, and promoter-related chromatin structure. Results: CSC and WPC decreased global H4K16ac and H4K20me3 histone marks and mediated distinct and overlapping cancer-associated transcriptome signatures and pathway modulations that were cell line dependent and stratified across lung cancer cells in a histology-specific manner. Epiregulin encoding a master regulator of EGFR signaling that is overexpressed in lung cancers was up-regulated, whereas FILIP1L and ABI3BP encoding mediators of senescence that are repressed in lung cancers were down-regulated by CSC and WPC. Induction of epiregulin and repression of FILIP1L and ABI3BP by these condensates coincided with unique epigenetic alterations within the respective promoters. Conclusions: These findings support translational studies to ascertain if hookah-mediated epigenomic and transcriptomic alterations in cultured respiratory epithelia are detectable and clinically relevant in hookah smokers. |
topic |
Hookah smoke Cigarette smoke Respiratory epithelia EREG FILIP1L ABI3BP |
url |
http://www.sciencedirect.com/science/article/pii/S2666364321000400 |
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doaj-873846f098014644911c9514ccac2c842021-07-25T04:44:01ZengElsevierJTO Clinical and Research Reports2666-36432021-07-0127100181Hookah Smoke Mediates Cancer-Associated Epigenomic and Transcriptomic Signatures in Human Respiratory Epithelial CellsYin Xiong, PhD0Sichuan Xi, PhD1Sudheer Kumar Gara, PhD2Jigui Shan, PhD3James Gao, PhD4Mary Zhang, MS5Vivek Shukla, PhD6Ruihong Wang, PhD7Chuong D. Hoang, MD8Haobin Chen, MD, PhD9David S. Schrump, MD, MBA10Thoracic Epigenetics Section, Thoracic Surgery Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MarylandThoracic Epigenetics Section, Thoracic Surgery Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MarylandThoracic Epigenetics Section, Thoracic Surgery Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MarylandThe Biomedical Informatics and Data Science, Frederick National Laboratory for Cancer Research, Frederick, MarylandThoracic Epigenetics Section, Thoracic Surgery Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MarylandThoracic Epigenetics Section, Thoracic Surgery Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MarylandThoracic Epigenetics Section, Thoracic Surgery Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MarylandThoracic Epigenetics Section, Thoracic Surgery Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MarylandThoracic Epigenetics Section, Thoracic Surgery Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MarylandThoracic Epigenetics Section, Thoracic Surgery Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MarylandThoracic Epigenetics Section, Thoracic Surgery Branch, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland; Corresponding author Address for Correspondence: David S. Schrump, MD, MBA, Thoracic Epigenetics Section, Thoracic Surgery Branch, Center for Cancer Research, National Cancer Institute, Building 10, 4-3942, 10 Center Drive, Bethesda, MD 20892Introduction: Although communal smoking of hookah by means of water pipes is perceived to be a safe alternative to cigarette smoking, the effects of hookah smoke in respiratory epithelia have not been well characterized. This study evaluated epigenomic and transcriptomic effects of hookah smoke relative to cigarette smoke in human respiratory epithelial cells. Methods: Primary normal human small airway epithelial cells from three donors and cdk4 and hTERT-immortalized small airway epithelial cells and human bronchial epithelial cells were cultured for 5 days in normal media with or without cigarette smoke condensates (CSCs) or water pipe condensates (WPCs). Cell count, immunoblot, RNA sequencing, quantitative real-time reverse-transcriptase polymerase chain reaction, methylation-specific polymerase chain reaction, and quantitative chromatin immunoprecipitation techniques were used to compare effects of hookah and cigarette smoke on cell proliferation, global histone marks, gene expression, and promoter-related chromatin structure. Results: CSC and WPC decreased global H4K16ac and H4K20me3 histone marks and mediated distinct and overlapping cancer-associated transcriptome signatures and pathway modulations that were cell line dependent and stratified across lung cancer cells in a histology-specific manner. Epiregulin encoding a master regulator of EGFR signaling that is overexpressed in lung cancers was up-regulated, whereas FILIP1L and ABI3BP encoding mediators of senescence that are repressed in lung cancers were down-regulated by CSC and WPC. Induction of epiregulin and repression of FILIP1L and ABI3BP by these condensates coincided with unique epigenetic alterations within the respective promoters. Conclusions: These findings support translational studies to ascertain if hookah-mediated epigenomic and transcriptomic alterations in cultured respiratory epithelia are detectable and clinically relevant in hookah smokers.http://www.sciencedirect.com/science/article/pii/S2666364321000400Hookah smokeCigarette smokeRespiratory epitheliaEREGFILIP1LABI3BP |