A Bacteriophage DNA Mimic Protein Employs a Non-specific Strategy to Inhibit the Bacterial RNA Polymerase
DNA mimicry by proteins is a strategy that employed by some proteins to occupy the binding sites of the DNA-binding proteins and deny further access to these sites by DNA. Such proteins have been found in bacteriophage, eukaryotic virus, prokaryotic, and eukaryotic cells to imitate non-coding functi...
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Frontiers Media S.A.
2021-06-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.692512/full |
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doaj-874cbe9f37da4d65834efeec2a5e52402021-06-02T05:45:46ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-06-011210.3389/fmicb.2021.692512692512A Bacteriophage DNA Mimic Protein Employs a Non-specific Strategy to Inhibit the Bacterial RNA PolymeraseZhihao Wang0Zhihao Wang1Hongliang Wang2Nancy Mulvenna3Maximo Sanz-Hernandez4Peipei Zhang5Yanqing Li6Jia Ma7Yawen Wang8Steve Matthews9Sivaramesh Wigneshweraraj10Bing Liu11Bing Liu12BioBank, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, ChinaMRC Centre for Molecular Bacteriology and Infection, Imperial College London, London, United KingdomDepartment of Pathogen Biology and Immunology, School of Basic Medical Sciences, Xi’an Jiaotong University, Xi’an, ChinaMRC Centre for Molecular Bacteriology and Infection, Imperial College London, London, United KingdomMRC Centre for Molecular Bacteriology and Infection, Imperial College London, London, United KingdomBioBank, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, ChinaBioBank, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, ChinaBioBank, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, ChinaBioBank, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, ChinaMRC Centre for Molecular Bacteriology and Infection, Imperial College London, London, United KingdomMRC Centre for Molecular Bacteriology and Infection, Imperial College London, London, United KingdomBioBank, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, ChinaInstrument Analysis Centre of Xi’an Jiaotong University, Xi’an, ChinaDNA mimicry by proteins is a strategy that employed by some proteins to occupy the binding sites of the DNA-binding proteins and deny further access to these sites by DNA. Such proteins have been found in bacteriophage, eukaryotic virus, prokaryotic, and eukaryotic cells to imitate non-coding functions of DNA. Here, we report another phage protein Gp44 from bacteriophage SPO1 of Bacillus subtilis, employing mimicry as part of unusual strategy to inhibit host RNA polymerase. Consisting of three simple domains, Gp44 contains a DNA binding motif, a flexible DNA mimic domain and a random-coiled domain. Gp44 is able to anchor to host genome and interact bacterial RNA polymerase via the β and β′ subunit, resulting in bacterial growth inhibition. Our findings represent a non-specific strategy that SPO1 phage uses to target different bacterial transcription machinery regardless of the structural variations of RNA polymerases. This feature may have potential applications like generation of genetic engineered phages with Gp44 gene incorporated used in phage therapy to target a range of bacterial hosts.https://www.frontiersin.org/articles/10.3389/fmicb.2021.692512/fullbacteriophageRNA polymeraseDNA binding abilityintrinsic disordered proteinDNA mimic protein |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Zhihao Wang Zhihao Wang Hongliang Wang Nancy Mulvenna Maximo Sanz-Hernandez Peipei Zhang Yanqing Li Jia Ma Yawen Wang Steve Matthews Sivaramesh Wigneshweraraj Bing Liu Bing Liu |
| spellingShingle |
Zhihao Wang Zhihao Wang Hongliang Wang Nancy Mulvenna Maximo Sanz-Hernandez Peipei Zhang Yanqing Li Jia Ma Yawen Wang Steve Matthews Sivaramesh Wigneshweraraj Bing Liu Bing Liu A Bacteriophage DNA Mimic Protein Employs a Non-specific Strategy to Inhibit the Bacterial RNA Polymerase Frontiers in Microbiology bacteriophage RNA polymerase DNA binding ability intrinsic disordered protein DNA mimic protein |
| author_facet |
Zhihao Wang Zhihao Wang Hongliang Wang Nancy Mulvenna Maximo Sanz-Hernandez Peipei Zhang Yanqing Li Jia Ma Yawen Wang Steve Matthews Sivaramesh Wigneshweraraj Bing Liu Bing Liu |
| author_sort |
Zhihao Wang |
| title |
A Bacteriophage DNA Mimic Protein Employs a Non-specific Strategy to Inhibit the Bacterial RNA Polymerase |
| title_short |
A Bacteriophage DNA Mimic Protein Employs a Non-specific Strategy to Inhibit the Bacterial RNA Polymerase |
| title_full |
A Bacteriophage DNA Mimic Protein Employs a Non-specific Strategy to Inhibit the Bacterial RNA Polymerase |
| title_fullStr |
A Bacteriophage DNA Mimic Protein Employs a Non-specific Strategy to Inhibit the Bacterial RNA Polymerase |
| title_full_unstemmed |
A Bacteriophage DNA Mimic Protein Employs a Non-specific Strategy to Inhibit the Bacterial RNA Polymerase |
| title_sort |
bacteriophage dna mimic protein employs a non-specific strategy to inhibit the bacterial rna polymerase |
| publisher |
Frontiers Media S.A. |
| series |
Frontiers in Microbiology |
| issn |
1664-302X |
| publishDate |
2021-06-01 |
| description |
DNA mimicry by proteins is a strategy that employed by some proteins to occupy the binding sites of the DNA-binding proteins and deny further access to these sites by DNA. Such proteins have been found in bacteriophage, eukaryotic virus, prokaryotic, and eukaryotic cells to imitate non-coding functions of DNA. Here, we report another phage protein Gp44 from bacteriophage SPO1 of Bacillus subtilis, employing mimicry as part of unusual strategy to inhibit host RNA polymerase. Consisting of three simple domains, Gp44 contains a DNA binding motif, a flexible DNA mimic domain and a random-coiled domain. Gp44 is able to anchor to host genome and interact bacterial RNA polymerase via the β and β′ subunit, resulting in bacterial growth inhibition. Our findings represent a non-specific strategy that SPO1 phage uses to target different bacterial transcription machinery regardless of the structural variations of RNA polymerases. This feature may have potential applications like generation of genetic engineered phages with Gp44 gene incorporated used in phage therapy to target a range of bacterial hosts. |
| topic |
bacteriophage RNA polymerase DNA binding ability intrinsic disordered protein DNA mimic protein |
| url |
https://www.frontiersin.org/articles/10.3389/fmicb.2021.692512/full |
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