Diurnal Differences in Human Muscle Isometric Force In Vivo Are Associated with Differential Phosphorylation of Sarcomeric M-Band Proteins

• Main Authors: Zulezwan Ab Ab Malik, Kelly A. Bowden Davies, Elliott C. R. Hall, Jennifer Barrett, Samuel A. Pullinger, Robert M. Erskine, Sam O. Shepherd, Zafar Iqbal, Ben J. Edwards, Jatin G. Burniston
• Format: Article
• Language: English
• Published: MDPI AG 2020-08-01
• Series: Proteomes
• Subjects: maximum voluntary isometric contraction; muscle contraction; phosphopeptide; phosphoproteomic; protein processing; post-translational
• Online Access: https://www.mdpi.com/2227-7382/8/3/22

We investigated whether diurnal differences in muscle force output are associated with the post-translational state of muscle proteins. Ten physically active men (mean ± SD; age 26.7 ± 3.7 y) performed experimental sessions in the morning (08:00 h) and evening (17:00 h), which were counterbalanced in order of administration and separated by at least 72 h. Knee extensor maximal voluntary isometric contraction (MVIC) force and peak rate of force development (RFD) were measured, and samples of vastus lateralis were collected immediately after exercise. MVIC force was greater in the evening (mean difference of 67 N, 10.2%; <em>p</em> < 0.05). Two-dimensional (2D) gel analysis encompassed 122 proteoforms and discovered 6 significant (<em>p </em>< 0.05; false discovery rate [FDR] = 10%) diurnal differences. Phosphopeptide analysis identified 1693 phosphopeptides and detected 140 phosphopeptides from 104 proteins that were more (<em>p </em>< 0.05, FDR = 22%) phosphorylated in the morning. Myomesin 2, muscle creatine kinase, and the C-terminus of titin exhibited the most robust (FDR < 10%) diurnal differences. Exercise in the morning, compared to the evening, coincided with a greater phosphorylation of M-band-associated proteins in human muscle. These protein modifications may alter the M-band structure and disrupt force transmission, thus potentially explaining the lower force output in the morning.