Analytical strategy for determination of chloramphenicol in different biological matrices by liquid chromatography - mass spectrometry
Introduction: The main problem in determination of chloramphenicol in food of animal origin is a large number of matrices. The main target of this study was to create a method for determination and confirmation of chloramphenicol in products and food of animal origin. Material and Methods: Each 5 g...
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doaj-87622149c0444e74bb32b03b4fb0d27b2021-09-06T19:40:55ZengSciendoJournal of Veterinary Research2450-86082017-09-0161332132710.1515/jvetres-2017-0032jvetres-2017-0032Analytical strategy for determination of chloramphenicol in different biological matrices by liquid chromatography - mass spectrometryŚniegocki Tomasz0Gbylik-Sikorska Małgorzata1Posyniak Andrzej2Department of Pharmacology and Toxicology National Veterinary Research Institute, 24-100 Pulawy, PolandDepartment of Pharmacology and Toxicology National Veterinary Research Institute, 24-100 Pulawy, PolandDepartment of Pharmacology and Toxicology National Veterinary Research Institute, 24-100 Pulawy, PolandIntroduction: The main problem in determination of chloramphenicol in food of animal origin is a large number of matrices. The main target of this study was to create a method for determination and confirmation of chloramphenicol in products and food of animal origin. Material and Methods: Each 5 g matrix sample was mixed with 5 mL of water and 10 mL of acetonitrile/ethyl acetate, homogenised, and centrifuged. The organic layer was evaporated and redissolved in 6 mL of 4% NaCl. The extract was cleaned up by SPE technique. Chloramphenicol was analysed by LC-MS/MS in electrospray mode. Results: The procedure was validated according to the Commission Decision No. 2002/657/EC. The apparent recoveries were in the range of 92.1% to 107.1% with a repeatability less than 11.0% (4.4%-11.0%) and within-laboratory reproducibility below 13.6% (4.7%-13.6%). Conclusion: The method was successfully validated and proved to be efficient, precise, and useful for quantification of chloramphenicol in more than 20 different matrices.https://doi.org/10.1515/jvetres-2017-0032food of animal originchloramphenicolresiduesanalytical method |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Śniegocki Tomasz Gbylik-Sikorska Małgorzata Posyniak Andrzej |
spellingShingle |
Śniegocki Tomasz Gbylik-Sikorska Małgorzata Posyniak Andrzej Analytical strategy for determination of chloramphenicol in different biological matrices by liquid chromatography - mass spectrometry Journal of Veterinary Research food of animal origin chloramphenicol residues analytical method |
author_facet |
Śniegocki Tomasz Gbylik-Sikorska Małgorzata Posyniak Andrzej |
author_sort |
Śniegocki Tomasz |
title |
Analytical strategy for determination of chloramphenicol in different biological matrices by liquid chromatography - mass spectrometry |
title_short |
Analytical strategy for determination of chloramphenicol in different biological matrices by liquid chromatography - mass spectrometry |
title_full |
Analytical strategy for determination of chloramphenicol in different biological matrices by liquid chromatography - mass spectrometry |
title_fullStr |
Analytical strategy for determination of chloramphenicol in different biological matrices by liquid chromatography - mass spectrometry |
title_full_unstemmed |
Analytical strategy for determination of chloramphenicol in different biological matrices by liquid chromatography - mass spectrometry |
title_sort |
analytical strategy for determination of chloramphenicol in different biological matrices by liquid chromatography - mass spectrometry |
publisher |
Sciendo |
series |
Journal of Veterinary Research |
issn |
2450-8608 |
publishDate |
2017-09-01 |
description |
Introduction: The main problem in determination of chloramphenicol in food of animal origin is a large number of matrices. The main target of this study was to create a method for determination and confirmation of chloramphenicol in products and food of animal origin. Material and Methods: Each 5 g matrix sample was mixed with 5 mL of water and 10 mL of acetonitrile/ethyl acetate, homogenised, and centrifuged. The organic layer was evaporated and redissolved in 6 mL of 4% NaCl. The extract was cleaned up by SPE technique. Chloramphenicol was analysed by LC-MS/MS in electrospray mode. Results: The procedure was validated according to the Commission Decision No. 2002/657/EC. The apparent recoveries were in the range of 92.1% to 107.1% with a repeatability less than 11.0% (4.4%-11.0%) and within-laboratory reproducibility below 13.6% (4.7%-13.6%). Conclusion: The method was successfully validated and proved to be efficient, precise, and useful for quantification of chloramphenicol in more than 20 different matrices. |
topic |
food of animal origin chloramphenicol residues analytical method |
url |
https://doi.org/10.1515/jvetres-2017-0032 |
work_keys_str_mv |
AT sniegockitomasz analyticalstrategyfordeterminationofchloramphenicolindifferentbiologicalmatricesbyliquidchromatographymassspectrometry AT gbyliksikorskamałgorzata analyticalstrategyfordeterminationofchloramphenicolindifferentbiologicalmatricesbyliquidchromatographymassspectrometry AT posyniakandrzej analyticalstrategyfordeterminationofchloramphenicolindifferentbiologicalmatricesbyliquidchromatographymassspectrometry |
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