Analytical strategy for determination of chloramphenicol in different biological matrices by liquid chromatography - mass spectrometry

• Main Authors: Śniegocki Tomasz, Gbylik-Sikorska Małgorzata, Posyniak Andrzej
• Format: Article
• Language: English
• Published: Sciendo 2017-09-01
• Series: Journal of Veterinary Research
• Subjects: food of animal origin; chloramphenicol; residues; analytical method
• Online Access: https://doi.org/10.1515/jvetres-2017-0032

Introduction: The main problem in determination of chloramphenicol in food of animal origin is a large number of matrices. The main target of this study was to create a method for determination and confirmation of chloramphenicol in products and food of animal origin. Material and Methods: Each 5 g matrix sample was mixed with 5 mL of water and 10 mL of acetonitrile/ethyl acetate, homogenised, and centrifuged. The organic layer was evaporated and redissolved in 6 mL of 4% NaCl. The extract was cleaned up by SPE technique. Chloramphenicol was analysed by LC-MS/MS in electrospray mode. Results: The procedure was validated according to the Commission Decision No. 2002/657/EC. The apparent recoveries were in the range of 92.1% to 107.1% with a repeatability less than 11.0% (4.4%-11.0%) and within-laboratory reproducibility below 13.6% (4.7%-13.6%). Conclusion: The method was successfully validated and proved to be efficient, precise, and useful for quantification of chloramphenicol in more than 20 different matrices.