Changes of PBP5 Gene Expression in Enterococcal Isolates from Renal Transplantation Recipients

Changes of PBP5 Gene Expression in Enterococcal Isolates from Renal Transplantation Recipients

The aim of the study was to evaluate changes in expression of PBP5 gene associated with immunosuppression. A linear locked nucleic acid (LNA) probe was used to measure resistance gene expression by the Flow-FISH method. Expression of the PBP5 gene measured by Flow-FISH was higher in enterococcal st...

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Main Authors: T. Jarzembowski, A. Daca, J. Witkowski, B. Rutkowski, J. Gołębiewska, A. Dębska-Ślizień
Format: Article
Language:English
Published: Hindawi Limited 2013-01-01
Series:BioMed Research International
Online Access:http://dx.doi.org/10.1155/2013/687156
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spelling doaj-876e0e005b514aa1854810ecc88731af2020-11-24T21:10:28ZengHindawi LimitedBioMed Research International2314-61332314-61412013-01-01201310.1155/2013/687156687156Changes of PBP5 Gene Expression in Enterococcal Isolates from Renal Transplantation RecipientsT. Jarzembowski0A. Daca1J. Witkowski2B. Rutkowski3J. Gołębiewska4A. Dębska-Ślizień5Department of Microbiology, Medical University of Gdańsk, Do Studzienki 38 Street, 80-227 Gdańsk, PolandDepartment of Pathophysiology, Medical University of Gdańsk, Dębinki 7 Street, 80-211 Gdańsk, PolandDepartment of Pathophysiology, Medical University of Gdańsk, Dębinki 7 Street, 80-211 Gdańsk, PolandDepartment of Nephrology, Transplantology and Internal Medicine, Medical University of Gdańsk, Dębinki 7 Street, 80-952 Gdańsk, PolandDepartment of Nephrology, Transplantology and Internal Medicine, Medical University of Gdańsk, Dębinki 7 Street, 80-952 Gdańsk, PolandDepartment of Nephrology, Transplantology and Internal Medicine, Medical University of Gdańsk, Dębinki 7 Street, 80-952 Gdańsk, PolandThe aim of the study was to evaluate changes in expression of PBP5 gene associated with immunosuppression. A linear locked nucleic acid (LNA) probe was used to measure resistance gene expression by the Flow-FISH method. Expression of the PBP5 gene measured by Flow-FISH was higher in enterococcal strains isolated from renal transplantation (RTx) recipients than in commensal strains. Additionally, in contrast to commensal strains in isolates from RTx patients, PBP5 gene expression was 17.45% higher in biofilms than in planktonic cells. Detailed comparison also showed that cyclosporine seemed to induce higher expression of PBP5 as compared to tacrolimus.http://dx.doi.org/10.1155/2013/687156
collection DOAJ
language English
format Article
sources DOAJ
author T. Jarzembowski
A. Daca
J. Witkowski
B. Rutkowski
J. Gołębiewska
A. Dębska-Ślizień
spellingShingle T. Jarzembowski
A. Daca
J. Witkowski
B. Rutkowski
J. Gołębiewska
A. Dębska-Ślizień
Changes of PBP5 Gene Expression in Enterococcal Isolates from Renal Transplantation Recipients
BioMed Research International
author_facet T. Jarzembowski
A. Daca
J. Witkowski
B. Rutkowski
J. Gołębiewska
A. Dębska-Ślizień
author_sort T. Jarzembowski
title Changes of PBP5 Gene Expression in Enterococcal Isolates from Renal Transplantation Recipients
title_short Changes of PBP5 Gene Expression in Enterococcal Isolates from Renal Transplantation Recipients
title_full Changes of PBP5 Gene Expression in Enterococcal Isolates from Renal Transplantation Recipients
title_fullStr Changes of PBP5 Gene Expression in Enterococcal Isolates from Renal Transplantation Recipients
title_full_unstemmed Changes of PBP5 Gene Expression in Enterococcal Isolates from Renal Transplantation Recipients
title_sort changes of pbp5 gene expression in enterococcal isolates from renal transplantation recipients
publisher Hindawi Limited
series BioMed Research International
issn 2314-6133
2314-6141
publishDate 2013-01-01
description The aim of the study was to evaluate changes in expression of PBP5 gene associated with immunosuppression. A linear locked nucleic acid (LNA) probe was used to measure resistance gene expression by the Flow-FISH method. Expression of the PBP5 gene measured by Flow-FISH was higher in enterococcal strains isolated from renal transplantation (RTx) recipients than in commensal strains. Additionally, in contrast to commensal strains in isolates from RTx patients, PBP5 gene expression was 17.45% higher in biofilms than in planktonic cells. Detailed comparison also showed that cyclosporine seemed to induce higher expression of PBP5 as compared to tacrolimus.
url http://dx.doi.org/10.1155/2013/687156
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