Antioxidant activity of ethanolic extract from cultivated strawberries’ leaves (Fragariae folium)
Strawberry is a member of the rose family (Rosaceae, subfamily Rosoideae, tribe Potentilleae) in the genus Fragaria. The cultivated varieties of commercial strawberries usually were designated as Fragaria ananassa. Root, leaf, flower and fruit have the healing properties. The strawberry lea...
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Association of Chemical Engineers of Serbia
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doaj-877561be49064b70ab221f00bfa0b55e2020-11-24T22:48:54ZengAssociation of Chemical Engineers of SerbiaHemijska Industrija 0367-598X2217-74262015-01-0169556757610.2298/HEMIND140718077S0367-598X1400077SAntioxidant activity of ethanolic extract from cultivated strawberries’ leaves (Fragariae folium)Stanojević Ljiljana P.0Stanojević Jelena S.1Cvetković Dragan J.2Cakić Milorad D.3Ilić Dušica P.4Faculty of Technology, LeskovacFaculty of Technology, LeskovacFaculty of Technology, LeskovacFaculty of Technology, LeskovacFaculty of Technology, LeskovacStrawberry is a member of the rose family (Rosaceae, subfamily Rosoideae, tribe Potentilleae) in the genus Fragaria. The cultivated varieties of commercial strawberries usually were designated as Fragaria ananassa. Root, leaf, flower and fruit have the healing properties. The strawberry leaves extract is used for blood cleaning, for treatment of oral inflammation, diarrhea, various gastro-intestinal inflammation, and hemorrhoids, as well as a diuretic. So far, many positive biological effects of strawberries (anticancer, antioxidant and anticoagulant effect) have been proven. The purpose of this study was to evaluate the antioxidant potential of ethanolic extract from cultivated strawberries (Fragariae folium, varieties Senga Sengana) by using different antioxidant assays (DPPH, FRAP, FIC, H2O2 and TBA-MDA). Ethanolic extract from strawberry leaves was obtained by reflux extraction at the boiling temperature. Total phenols and total flavonoids content was determined spectrophotometrically by the method of Folin-Ciocalteu and by method with AlCl3, respectively. In the extract was determined high content of total phenols, while the total flavonoid content is much lower. The concentrations of extract required to neutralize 50% of the initial concentration of DPPH radicals (EC50) after 20 minutes incubation and immediately after adding DPPH radical solution were 7,91 and 19,46 μgcm-3, respectively. Extract was achieved the maximum iron ions chelating ability (67.89%) at a concentration of 2 mgcm-3. Inhibition of lipid peroxidation of 70% was achieved by extract concentration of 0.03125 mgcm-3, while the maximum neutralization of H2O2 (30.47%) was achieved by extract concentration of 0.5 mgcm-3. FRAP value of the investigated extract is 284.51 mgFe/g of dry extract. Presented results of the antioxidant activity show that the obtained extract from the cultivated strawberry leaves is a potential source of natural antioxidants. [Projekat Ministarstva nauke Republike Srbije, br. TR 34012: Biljni i sintetski bioaktivni proizvodi novije generacije]http://www.doiserbia.nb.rs/img/doi/0367-598X/2015/0367-598X1400077S.pdfStrawberry leaves (Fragariae folium)Senga senganatotal phenolstotal flavonoidsantioxidant activity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Stanojević Ljiljana P. Stanojević Jelena S. Cvetković Dragan J. Cakić Milorad D. Ilić Dušica P. |
spellingShingle |
Stanojević Ljiljana P. Stanojević Jelena S. Cvetković Dragan J. Cakić Milorad D. Ilić Dušica P. Antioxidant activity of ethanolic extract from cultivated strawberries’ leaves (Fragariae folium) Hemijska Industrija Strawberry leaves (Fragariae folium) Senga sengana total phenols total flavonoids antioxidant activity |
author_facet |
Stanojević Ljiljana P. Stanojević Jelena S. Cvetković Dragan J. Cakić Milorad D. Ilić Dušica P. |
author_sort |
Stanojević Ljiljana P. |
title |
Antioxidant activity of ethanolic extract from cultivated strawberries’ leaves (Fragariae folium) |
title_short |
Antioxidant activity of ethanolic extract from cultivated strawberries’ leaves (Fragariae folium) |
title_full |
Antioxidant activity of ethanolic extract from cultivated strawberries’ leaves (Fragariae folium) |
title_fullStr |
Antioxidant activity of ethanolic extract from cultivated strawberries’ leaves (Fragariae folium) |
title_full_unstemmed |
Antioxidant activity of ethanolic extract from cultivated strawberries’ leaves (Fragariae folium) |
title_sort |
antioxidant activity of ethanolic extract from cultivated strawberries’ leaves (fragariae folium) |
publisher |
Association of Chemical Engineers of Serbia |
series |
Hemijska Industrija |
issn |
0367-598X 2217-7426 |
publishDate |
2015-01-01 |
description |
Strawberry is a member of the rose family (Rosaceae, subfamily Rosoideae,
tribe Potentilleae) in the genus Fragaria. The cultivated varieties of
commercial strawberries usually were designated as Fragaria ananassa. Root,
leaf, flower and fruit have the healing properties. The strawberry leaves
extract is used for blood cleaning, for treatment of oral inflammation,
diarrhea, various gastro-intestinal inflammation, and hemorrhoids, as well as
a diuretic. So far, many positive biological effects of strawberries
(anticancer, antioxidant and anticoagulant effect) have been proven. The
purpose of this study was to evaluate the antioxidant potential of ethanolic
extract from cultivated strawberries (Fragariae folium, varieties Senga
Sengana) by using different antioxidant assays (DPPH, FRAP, FIC, H2O2 and
TBA-MDA). Ethanolic extract from strawberry leaves was obtained by reflux
extraction at the boiling temperature. Total phenols and total flavonoids
content was determined spectrophotometrically by the method of
Folin-Ciocalteu and by method with AlCl3, respectively. In the extract was
determined high content of total phenols, while the total flavonoid content
is much lower. The concentrations of extract required to neutralize 50% of
the initial concentration of DPPH radicals (EC50) after 20 minutes incubation
and immediately after adding DPPH radical solution were 7,91 and 19,46
μgcm-3, respectively. Extract was achieved the maximum iron ions chelating
ability (67.89%) at a concentration of 2 mgcm-3. Inhibition of lipid
peroxidation of 70% was achieved by extract concentration of 0.03125 mgcm-3,
while the maximum neutralization of H2O2 (30.47%) was achieved by extract
concentration of 0.5 mgcm-3. FRAP value of the investigated extract is 284.51
mgFe/g of dry extract. Presented results of the antioxidant activity show
that the obtained extract from the cultivated strawberry leaves is a
potential source of natural antioxidants. [Projekat Ministarstva nauke
Republike Srbije, br. TR 34012: Biljni i sintetski bioaktivni proizvodi
novije generacije] |
topic |
Strawberry leaves (Fragariae folium) Senga sengana total phenols total flavonoids antioxidant activity |
url |
http://www.doiserbia.nb.rs/img/doi/0367-598X/2015/0367-598X1400077S.pdf |
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