BLIMP1 Is Required for Postnatal Epidermal Homeostasis but Does Not Define a Sebaceous Gland Progenitor under Steady-State Conditions
B-lymphocyte-induced nuclear maturation protein 1 (BLIMP1) was previously reported to define a sebaceous gland (SG) progenitor population in the epidermis. However, the recent identification of multiple stem cell populations in the hair follicle junctional zone has led us to re-evaluate its function...
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doaj-87761270245f4670ac108e702a4347592020-11-24T23:18:06ZengElsevierStem Cell Reports2213-67112014-10-013462063310.1016/j.stemcr.2014.08.007BLIMP1 Is Required for Postnatal Epidermal Homeostasis but Does Not Define a Sebaceous Gland Progenitor under Steady-State ConditionsKai Kretzschmar0Denny L. Cottle1Giacomo Donati2Ming-Feng Chiang3Sven R. Quist4Harald P. Gollnick5Ken Natsuga6Kuo-I Lin7Fiona M. Watt8Centre for Stem Cells and Regenerative Medicine, King’s College London, Guy’s Hospital, 28th Floor Tower Wing, Great Maze Pond, London SE1 9RT, UKWellcome Trust-Medical Research Council Stem Cell Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UKCentre for Stem Cells and Regenerative Medicine, King’s College London, Guy’s Hospital, 28th Floor Tower Wing, Great Maze Pond, London SE1 9RT, UKGraduate Institute of Life Sciences, National Defense Medical Centre, Taipei 114, Taiwan, ROCEpithelial Cell Biology Laboratory, Cancer Research UK Cambridge Research Institute, Li Ka Shing Centre, Robinson Way, Cambridge CB2 0RE, UKClinic of Dermatology and Venereology, Otto-von-Guericke University, Magdeburg, Leipziger Strasse 44, 39120 Magdeburg, GermanyEpithelial Cell Biology Laboratory, Cancer Research UK Cambridge Research Institute, Li Ka Shing Centre, Robinson Way, Cambridge CB2 0RE, UKGraduate Institute of Life Sciences, National Defense Medical Centre, Taipei 114, Taiwan, ROCCentre for Stem Cells and Regenerative Medicine, King’s College London, Guy’s Hospital, 28th Floor Tower Wing, Great Maze Pond, London SE1 9RT, UKB-lymphocyte-induced nuclear maturation protein 1 (BLIMP1) was previously reported to define a sebaceous gland (SG) progenitor population in the epidermis. However, the recent identification of multiple stem cell populations in the hair follicle junctional zone has led us to re-evaluate its function. We show, in agreement with previous studies, that BLIMP1 is expressed by postmitotic, terminally differentiated epidermal cells within the SG, interfollicular epidermis, and hair follicle. Epidermal overexpression of c-Myc results in loss of BLIMP1+ cells, an effect modulated by androgen signaling. Epidermal-specific deletion of Blimp1 causes multiple differentiation defects in the epidermis in addition to SG enlargement. In culture, BLIMP1+ sebocytes have no greater clonogenic potential than BLIMP1− sebocytes. Finally, lineage-tracing experiments reveal that, under steady-state conditions, BLIMP1-expressing cells do not divide. Thus, rather than defining a sebocyte progenitor population, BLIMP1 functions in terminally differentiated cells to maintain homeostasis in multiple epidermal compartments.http://www.sciencedirect.com/science/article/pii/S2213671114002616 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kai Kretzschmar Denny L. Cottle Giacomo Donati Ming-Feng Chiang Sven R. Quist Harald P. Gollnick Ken Natsuga Kuo-I Lin Fiona M. Watt |
spellingShingle |
Kai Kretzschmar Denny L. Cottle Giacomo Donati Ming-Feng Chiang Sven R. Quist Harald P. Gollnick Ken Natsuga Kuo-I Lin Fiona M. Watt BLIMP1 Is Required for Postnatal Epidermal Homeostasis but Does Not Define a Sebaceous Gland Progenitor under Steady-State Conditions Stem Cell Reports |
author_facet |
Kai Kretzschmar Denny L. Cottle Giacomo Donati Ming-Feng Chiang Sven R. Quist Harald P. Gollnick Ken Natsuga Kuo-I Lin Fiona M. Watt |
author_sort |
Kai Kretzschmar |
title |
BLIMP1 Is Required for Postnatal Epidermal Homeostasis but Does Not Define a Sebaceous Gland Progenitor under Steady-State Conditions |
title_short |
BLIMP1 Is Required for Postnatal Epidermal Homeostasis but Does Not Define a Sebaceous Gland Progenitor under Steady-State Conditions |
title_full |
BLIMP1 Is Required for Postnatal Epidermal Homeostasis but Does Not Define a Sebaceous Gland Progenitor under Steady-State Conditions |
title_fullStr |
BLIMP1 Is Required for Postnatal Epidermal Homeostasis but Does Not Define a Sebaceous Gland Progenitor under Steady-State Conditions |
title_full_unstemmed |
BLIMP1 Is Required for Postnatal Epidermal Homeostasis but Does Not Define a Sebaceous Gland Progenitor under Steady-State Conditions |
title_sort |
blimp1 is required for postnatal epidermal homeostasis but does not define a sebaceous gland progenitor under steady-state conditions |
publisher |
Elsevier |
series |
Stem Cell Reports |
issn |
2213-6711 |
publishDate |
2014-10-01 |
description |
B-lymphocyte-induced nuclear maturation protein 1 (BLIMP1) was previously reported to define a sebaceous gland (SG) progenitor population in the epidermis. However, the recent identification of multiple stem cell populations in the hair follicle junctional zone has led us to re-evaluate its function. We show, in agreement with previous studies, that BLIMP1 is expressed by postmitotic, terminally differentiated epidermal cells within the SG, interfollicular epidermis, and hair follicle. Epidermal overexpression of c-Myc results in loss of BLIMP1+ cells, an effect modulated by androgen signaling. Epidermal-specific deletion of Blimp1 causes multiple differentiation defects in the epidermis in addition to SG enlargement. In culture, BLIMP1+ sebocytes have no greater clonogenic potential than BLIMP1− sebocytes. Finally, lineage-tracing experiments reveal that, under steady-state conditions, BLIMP1-expressing cells do not divide. Thus, rather than defining a sebocyte progenitor population, BLIMP1 functions in terminally differentiated cells to maintain homeostasis in multiple epidermal compartments. |
url |
http://www.sciencedirect.com/science/article/pii/S2213671114002616 |
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