Metal-Carbon Interactions on Reduced Graphene Oxide under Facile Thermal Treatment: Microbiological and Cell Assay
Silver-functionalized reduced graphene oxide (Ag-rGO) nanosheets were prepared by single chemical and thermal processes, with very low concentration of silver. The resulting carbon framework consists of reduced graphene oxide (rGO) sheets or 3D networks, decorated with anchored silver nanoparticles....
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doaj-87772073355a449e935909085748bcd22020-11-24T22:50:34ZengHindawi LimitedJournal of Nanomaterials1687-41101687-41292017-01-01201710.1155/2017/60595406059540Metal-Carbon Interactions on Reduced Graphene Oxide under Facile Thermal Treatment: Microbiological and Cell AssayN. L. V. Carreño0A. M. Barbosa1V. C. Duarte2C. F. Correa3C. Ferrúa4F. Nedel5S. Peralta6C. A. Mills7R. Rhodes8F. L. M. Sam9S. R. P. Silva10Graduate Program: Materials Science and Engineering, Technology Development Center, Federal University of Pelotas, 96010-610 Pelotas, RS, BrazilGraduate Program: Materials Science and Engineering, Technology Development Center, Federal University of Pelotas, 96010-610 Pelotas, RS, BrazilGraduate Program: Materials Science and Engineering, Technology Development Center, Federal University of Pelotas, 96010-610 Pelotas, RS, BrazilGraduate Program: Materials Science and Engineering, Technology Development Center, Federal University of Pelotas, 96010-610 Pelotas, RS, BrazilPost-Graduate Program in Health and Behavior, Catholic University of Pelotas, Pelotas, RS, BrazilPost-Graduate Program in Health and Behavior, Catholic University of Pelotas, Pelotas, RS, BrazilSchool of Dentistry, Federal University of Pelotas, 96010-610 Pelotas, RS, BrazilNano-Electronics Centre, Advanced Technology Institute (ATI), University of Surrey, Guildford GU2 7XH, UKNano-Electronics Centre, Advanced Technology Institute (ATI), University of Surrey, Guildford GU2 7XH, UKNano-Electronics Centre, Advanced Technology Institute (ATI), University of Surrey, Guildford GU2 7XH, UKNano-Electronics Centre, Advanced Technology Institute (ATI), University of Surrey, Guildford GU2 7XH, UKSilver-functionalized reduced graphene oxide (Ag-rGO) nanosheets were prepared by single chemical and thermal processes, with very low concentration of silver. The resulting carbon framework consists of reduced graphene oxide (rGO) sheets or 3D networks, decorated with anchored silver nanoparticles. The Ag-rGO nanosheets were dispersed into a polymer matrix and the composites evaluated for use as biological scaffolds. The rGO material in poly(dimethylsiloxane) (PDMS) has been tested for antimicrobial activity against Gram-positive Staphylococcus aureus (S. Aureus) bacteria, after exposure times of 24 and 120 hours, as well as in the determination of cell viability on cultures of fibroblast cells (NIH/3T3). Using 1 mL of Ag-rGO in PDMS the antibacterial effectiveness against Staphylococcus aureus was limited, showing an increased amount of Colony Forming Units (CFU), after 24 hours of contact. In the cell viability assay, after 48 hours of contact, the group of 1 mL of Ag-rGO with PDMS was the only group that increased cell viability when compared to the control group. In this context, it is believed these behaviors are due to the increase in cell adhesion capacity promoted by the rGO. Thus, the Ag-rGO/PDMS hybrid nanocomposite films can be used as scaffolds for tissue engineering, as they limit antimicrobial activity.http://dx.doi.org/10.1155/2017/6059540 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
N. L. V. Carreño A. M. Barbosa V. C. Duarte C. F. Correa C. Ferrúa F. Nedel S. Peralta C. A. Mills R. Rhodes F. L. M. Sam S. R. P. Silva |
spellingShingle |
N. L. V. Carreño A. M. Barbosa V. C. Duarte C. F. Correa C. Ferrúa F. Nedel S. Peralta C. A. Mills R. Rhodes F. L. M. Sam S. R. P. Silva Metal-Carbon Interactions on Reduced Graphene Oxide under Facile Thermal Treatment: Microbiological and Cell Assay Journal of Nanomaterials |
author_facet |
N. L. V. Carreño A. M. Barbosa V. C. Duarte C. F. Correa C. Ferrúa F. Nedel S. Peralta C. A. Mills R. Rhodes F. L. M. Sam S. R. P. Silva |
author_sort |
N. L. V. Carreño |
title |
Metal-Carbon Interactions on Reduced Graphene Oxide under Facile Thermal Treatment: Microbiological and Cell Assay |
title_short |
Metal-Carbon Interactions on Reduced Graphene Oxide under Facile Thermal Treatment: Microbiological and Cell Assay |
title_full |
Metal-Carbon Interactions on Reduced Graphene Oxide under Facile Thermal Treatment: Microbiological and Cell Assay |
title_fullStr |
Metal-Carbon Interactions on Reduced Graphene Oxide under Facile Thermal Treatment: Microbiological and Cell Assay |
title_full_unstemmed |
Metal-Carbon Interactions on Reduced Graphene Oxide under Facile Thermal Treatment: Microbiological and Cell Assay |
title_sort |
metal-carbon interactions on reduced graphene oxide under facile thermal treatment: microbiological and cell assay |
publisher |
Hindawi Limited |
series |
Journal of Nanomaterials |
issn |
1687-4110 1687-4129 |
publishDate |
2017-01-01 |
description |
Silver-functionalized reduced graphene oxide (Ag-rGO) nanosheets were prepared by single chemical and thermal processes, with very low concentration of silver. The resulting carbon framework consists of reduced graphene oxide (rGO) sheets or 3D networks, decorated with anchored silver nanoparticles. The Ag-rGO nanosheets were dispersed into a polymer matrix and the composites evaluated for use as biological scaffolds. The rGO material in poly(dimethylsiloxane) (PDMS) has been tested for antimicrobial activity against Gram-positive Staphylococcus aureus (S. Aureus) bacteria, after exposure times of 24 and 120 hours, as well as in the determination of cell viability on cultures of fibroblast cells (NIH/3T3). Using 1 mL of Ag-rGO in PDMS the antibacterial effectiveness against Staphylococcus aureus was limited, showing an increased amount of Colony Forming Units (CFU), after 24 hours of contact. In the cell viability assay, after 48 hours of contact, the group of 1 mL of Ag-rGO with PDMS was the only group that increased cell viability when compared to the control group. In this context, it is believed these behaviors are due to the increase in cell adhesion capacity promoted by the rGO. Thus, the Ag-rGO/PDMS hybrid nanocomposite films can be used as scaffolds for tissue engineering, as they limit antimicrobial activity. |
url |
http://dx.doi.org/10.1155/2017/6059540 |
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