Benchtop Technologies for Circulating Tumor Cells Separation Based on Biophysical Properties
Circulating tumor cells (CTCs) are tumor cells that have detached from primary tumor site and are transported via the circulation system. The importance of CTCs as prognostic biomarker is leveraged when multiple studies found that patient with cutoff of 5 CTCs per 7.5 mL blood has poor survival rate...
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Online Access: | http://dx.doi.org/10.1155/2015/239362 |
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doaj-877a174fbac44e14b4ba50ab957a609a2020-11-25T00:02:57ZengHindawi LimitedBioMed Research International2314-61332314-61412015-01-01201510.1155/2015/239362239362Benchtop Technologies for Circulating Tumor Cells Separation Based on Biophysical PropertiesWan Shi Low0Wan Abu Bakar Wan Abas1Department of Biomedical Engineering, University of Malaya, 50603 Kuala Lumpur, MalaysiaDepartment of Biomedical Engineering, University of Malaya, 50603 Kuala Lumpur, MalaysiaCirculating tumor cells (CTCs) are tumor cells that have detached from primary tumor site and are transported via the circulation system. The importance of CTCs as prognostic biomarker is leveraged when multiple studies found that patient with cutoff of 5 CTCs per 7.5 mL blood has poor survival rate. Despite its clinical relevance, the isolation and characterization of CTCs can be quite challenging due to their large morphological variability and the rare presence of CTCs within the blood. Numerous methods have been employed and discussed in the literature for CTCs separation. In this paper, we will focus on label free CTCs isolation methods, in which the biophysical and biomechanical properties of cells (e.g., size, deformability, and electricity) are exploited for CTCs detection. To assess the present state of various isolation methods, key performance metrics such as capture efficiency, cell viability, and throughput will be reported. Finally, we discuss the challenges and future perspectives of CTC isolation technologies.http://dx.doi.org/10.1155/2015/239362 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wan Shi Low Wan Abu Bakar Wan Abas |
spellingShingle |
Wan Shi Low Wan Abu Bakar Wan Abas Benchtop Technologies for Circulating Tumor Cells Separation Based on Biophysical Properties BioMed Research International |
author_facet |
Wan Shi Low Wan Abu Bakar Wan Abas |
author_sort |
Wan Shi Low |
title |
Benchtop Technologies for Circulating Tumor Cells Separation Based on Biophysical Properties |
title_short |
Benchtop Technologies for Circulating Tumor Cells Separation Based on Biophysical Properties |
title_full |
Benchtop Technologies for Circulating Tumor Cells Separation Based on Biophysical Properties |
title_fullStr |
Benchtop Technologies for Circulating Tumor Cells Separation Based on Biophysical Properties |
title_full_unstemmed |
Benchtop Technologies for Circulating Tumor Cells Separation Based on Biophysical Properties |
title_sort |
benchtop technologies for circulating tumor cells separation based on biophysical properties |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2015-01-01 |
description |
Circulating tumor cells (CTCs) are tumor cells that have detached from primary tumor site and are transported via the circulation system. The importance of CTCs as prognostic biomarker is leveraged when multiple studies found that patient with cutoff of 5 CTCs per 7.5 mL blood has poor survival rate. Despite its clinical relevance, the isolation and characterization of CTCs can be quite challenging due to their large morphological variability and the rare presence of CTCs within the blood. Numerous methods have been employed and discussed in the literature for CTCs separation. In this paper, we will focus on label free CTCs isolation methods, in which the biophysical and biomechanical properties of cells (e.g., size, deformability, and electricity) are exploited for CTCs detection. To assess the present state of various isolation methods, key performance metrics such as capture efficiency, cell viability, and throughput will be reported. Finally, we discuss the challenges and future perspectives of CTC isolation technologies. |
url |
http://dx.doi.org/10.1155/2015/239362 |
work_keys_str_mv |
AT wanshilow benchtoptechnologiesforcirculatingtumorcellsseparationbasedonbiophysicalproperties AT wanabubakarwanabas benchtoptechnologiesforcirculatingtumorcellsseparationbasedonbiophysicalproperties |
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