A superficial hyperechoic band in human articular cartilage on ultrasonography with histological correlation: preliminary observations
Purpose: To demonstrate the superficial hyperechoic band (SHEB) in articular cartilage by using ultrasonography (US) and to assess its correlation with histological images. Methods: In total, 47 regions of interest (ROIs) were analyzed from six tibial osteochondral specimens (OCSs) that were obtaine...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Korean Society of Ultrasound in Medicine
2015-04-01
|
Series: | Ultrasonography |
Subjects: | |
Online Access: | http://www.e-ultrasonography.org/upload/usg-14047.pdf |
id |
doaj-87841e6bfda14bd69f37c9c713aa6cba |
---|---|
record_format |
Article |
spelling |
doaj-87841e6bfda14bd69f37c9c713aa6cba2020-11-25T01:05:23ZengKorean Society of Ultrasound in MedicineUltrasonography2288-59192288-59432015-04-0134211512410.14366/usg.1404769A superficial hyperechoic band in human articular cartilage on ultrasonography with histological correlation: preliminary observationsTae Sun Han0Kyu-Sung Kwack1Sunghoon Park2Byoung-Hyun Min3Seung-Hyun Yoon4Hyun Young Lee5Kyi Beom Lee6 Department of Radiology, Ajou University School of Medicine, Suwon, Korea Department of Radiology, Ajou University School of Medicine, Suwon, Korea Department of Radiology, Ajou University School of Medicine, Suwon, Korea Department of Orthopedic Surgery, Ajou University School of Medicine, Suwon, Korea Cartilage Regeneration Center, Ajou University Medical Center, Suwon, Korea Regional Clinical Trial Center, Ajou University Medical Center, Suwon, Korea Department of Pathology, Ajou University School of Medicine, Suwon, KoreaPurpose: To demonstrate the superficial hyperechoic band (SHEB) in articular cartilage by using ultrasonography (US) and to assess its correlation with histological images. Methods: In total, 47 regions of interest (ROIs) were analyzed from six tibial osteochondral specimens (OCSs) that were obtained after total knee arthroplasty. Ultrasonograms were obtained for each OCS. Then, matching histological sections from all specimens were obtained for comparison with the ultrasonograms. Two types of histological staining were used: Safranin-O stain (SO) to identify glycosaminoglycans (GAG) and Masson’s trichrome stain (MT) to identify collagen. In step 1, two observers evaluated whether there was an SHEB in each ROI. In step 2, the two observers evaluated which histological staining method correlated better with the SHEB by using the ImageJ software. Results: In step 1 of the analysis, 20 out of 47 ROIs showed an SHEB (42.6%, kappa=0.579). Step 2 showed that the SHEB correlated significantly better with the topographical variation in stainability in SO staining, indicating the GAG distribution, than with MT staining, indicating the collagen distribution (P<0.05, kappa=0.722). Conclusion: The SHEB that is frequently seen in human articular cartilage on high-resolution US correlated better with variations in SO staining than with variations in MT staining. Thus, we suggest that a SHEB is predominantly related to changes in GAG. Identifying an SHEB by US is a promising method for assessing the thickness of articular cartilage or for monitoring early osteoarthritis.http://www.e-ultrasonography.org/upload/usg-14047.pdfCartilageUltrasonographyKnee jointHistology |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tae Sun Han Kyu-Sung Kwack Sunghoon Park Byoung-Hyun Min Seung-Hyun Yoon Hyun Young Lee Kyi Beom Lee |
spellingShingle |
Tae Sun Han Kyu-Sung Kwack Sunghoon Park Byoung-Hyun Min Seung-Hyun Yoon Hyun Young Lee Kyi Beom Lee A superficial hyperechoic band in human articular cartilage on ultrasonography with histological correlation: preliminary observations Ultrasonography Cartilage Ultrasonography Knee joint Histology |
author_facet |
Tae Sun Han Kyu-Sung Kwack Sunghoon Park Byoung-Hyun Min Seung-Hyun Yoon Hyun Young Lee Kyi Beom Lee |
author_sort |
Tae Sun Han |
title |
A superficial hyperechoic band in human articular cartilage on ultrasonography with histological correlation: preliminary observations |
title_short |
A superficial hyperechoic band in human articular cartilage on ultrasonography with histological correlation: preliminary observations |
title_full |
A superficial hyperechoic band in human articular cartilage on ultrasonography with histological correlation: preliminary observations |
title_fullStr |
A superficial hyperechoic band in human articular cartilage on ultrasonography with histological correlation: preliminary observations |
title_full_unstemmed |
A superficial hyperechoic band in human articular cartilage on ultrasonography with histological correlation: preliminary observations |
title_sort |
superficial hyperechoic band in human articular cartilage on ultrasonography with histological correlation: preliminary observations |
publisher |
Korean Society of Ultrasound in Medicine |
series |
Ultrasonography |
issn |
2288-5919 2288-5943 |
publishDate |
2015-04-01 |
description |
Purpose: To demonstrate the superficial hyperechoic band (SHEB) in articular cartilage by using ultrasonography (US) and to assess its correlation with histological images. Methods: In total, 47 regions of interest (ROIs) were analyzed from six tibial osteochondral specimens (OCSs) that were obtained after total knee arthroplasty. Ultrasonograms were obtained for each OCS. Then, matching histological sections from all specimens were obtained for comparison with the ultrasonograms. Two types of histological staining were used: Safranin-O stain (SO) to identify glycosaminoglycans (GAG) and Masson’s trichrome stain (MT) to identify collagen. In step 1, two observers evaluated whether there was an SHEB in each ROI. In step 2, the two observers evaluated which histological staining method correlated better with the SHEB by using the ImageJ software. Results: In step 1 of the analysis, 20 out of 47 ROIs showed an SHEB (42.6%, kappa=0.579). Step 2 showed that the SHEB correlated significantly better with the topographical variation in stainability in SO staining, indicating the GAG distribution, than with MT staining, indicating the collagen distribution (P<0.05, kappa=0.722). Conclusion: The SHEB that is frequently seen in human articular cartilage on high-resolution US correlated better with variations in SO staining than with variations in MT staining. Thus, we suggest that a SHEB is predominantly related to changes in GAG. Identifying an SHEB by US is a promising method for assessing the thickness of articular cartilage or for monitoring early osteoarthritis. |
topic |
Cartilage Ultrasonography Knee joint Histology |
url |
http://www.e-ultrasonography.org/upload/usg-14047.pdf |
work_keys_str_mv |
AT taesunhan asuperficialhyperechoicbandinhumanarticularcartilageonultrasonographywithhistologicalcorrelationpreliminaryobservations AT kyusungkwack asuperficialhyperechoicbandinhumanarticularcartilageonultrasonographywithhistologicalcorrelationpreliminaryobservations AT sunghoonpark asuperficialhyperechoicbandinhumanarticularcartilageonultrasonographywithhistologicalcorrelationpreliminaryobservations AT byounghyunmin asuperficialhyperechoicbandinhumanarticularcartilageonultrasonographywithhistologicalcorrelationpreliminaryobservations AT seunghyunyoon asuperficialhyperechoicbandinhumanarticularcartilageonultrasonographywithhistologicalcorrelationpreliminaryobservations AT hyunyounglee asuperficialhyperechoicbandinhumanarticularcartilageonultrasonographywithhistologicalcorrelationpreliminaryobservations AT kyibeomlee asuperficialhyperechoicbandinhumanarticularcartilageonultrasonographywithhistologicalcorrelationpreliminaryobservations AT taesunhan superficialhyperechoicbandinhumanarticularcartilageonultrasonographywithhistologicalcorrelationpreliminaryobservations AT kyusungkwack superficialhyperechoicbandinhumanarticularcartilageonultrasonographywithhistologicalcorrelationpreliminaryobservations AT sunghoonpark superficialhyperechoicbandinhumanarticularcartilageonultrasonographywithhistologicalcorrelationpreliminaryobservations AT byounghyunmin superficialhyperechoicbandinhumanarticularcartilageonultrasonographywithhistologicalcorrelationpreliminaryobservations AT seunghyunyoon superficialhyperechoicbandinhumanarticularcartilageonultrasonographywithhistologicalcorrelationpreliminaryobservations AT hyunyounglee superficialhyperechoicbandinhumanarticularcartilageonultrasonographywithhistologicalcorrelationpreliminaryobservations AT kyibeomlee superficialhyperechoicbandinhumanarticularcartilageonultrasonographywithhistologicalcorrelationpreliminaryobservations |
_version_ |
1725194797082214400 |