Mitochondrial localization of vitamin D receptor in human platelets and differentiated megakaryocytes.
BACKGROUND: Like other steroid hormones, vitamin D elicits both transcriptional events and rapid non genomic effects. Vitamin D receptor (VDR) localization and mechanisms of VDR-triggered non genomic responses are still controversial. Although anticoagulant effects of vitamin D have been reported an...
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doaj-8785f89e005948c1b8812f6b6cdec3332020-11-25T02:39:47ZengPublic Library of Science (PLoS)PLoS ONE1932-62032010-01-0151e867010.1371/journal.pone.0008670Mitochondrial localization of vitamin D receptor in human platelets and differentiated megakaryocytes.Francesca SilvagnoEnrico De VivoAngelo AttanasioValentina GalloGianna MazzuccoGianpiero PescarmonaBACKGROUND: Like other steroid hormones, vitamin D elicits both transcriptional events and rapid non genomic effects. Vitamin D receptor (VDR) localization and mechanisms of VDR-triggered non genomic responses are still controversial. Although anticoagulant effects of vitamin D have been reported and VDR signalling has been characterized in monocytes and vascular cells, nothing is known about VDR expression and functions in human platelets, anucleated fragments of megakaryocytes which are known targets of other steroids. METHODOLOGY/PRINCIPAL FINDINGS: In this study we characterized the expression and cellular localization of VDR in human platelets and in a megakaryocyte lineage. Human platelets and their TPA-differentiated precursors expressed a classical 50 kDa VDR protein, which increased with megakaryocytes maturation. By biochemical fractionation studies we demonstrated the presence of the receptor in the soluble and mitochondrial compartment of human platelets, and the observation was confirmed by immunoelectron microscopy analysis. Similar localization was found in mature megakaryocytes, where besides its classical nuclear localization the receptor was evident as soluble and mitochondria resident protein. CONCLUSIONS: The results reported here suggest that megakaryocytopoiesis and platelet activation, which are calcium-dependent events, might be modulated by a mitochondrial non genomic activity of VDR. These data open challenging future studies on VDR physiological role in platelets and more generally in mitochondria.http://europepmc.org/articles/PMC2809087?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Francesca Silvagno Enrico De Vivo Angelo Attanasio Valentina Gallo Gianna Mazzucco Gianpiero Pescarmona |
spellingShingle |
Francesca Silvagno Enrico De Vivo Angelo Attanasio Valentina Gallo Gianna Mazzucco Gianpiero Pescarmona Mitochondrial localization of vitamin D receptor in human platelets and differentiated megakaryocytes. PLoS ONE |
author_facet |
Francesca Silvagno Enrico De Vivo Angelo Attanasio Valentina Gallo Gianna Mazzucco Gianpiero Pescarmona |
author_sort |
Francesca Silvagno |
title |
Mitochondrial localization of vitamin D receptor in human platelets and differentiated megakaryocytes. |
title_short |
Mitochondrial localization of vitamin D receptor in human platelets and differentiated megakaryocytes. |
title_full |
Mitochondrial localization of vitamin D receptor in human platelets and differentiated megakaryocytes. |
title_fullStr |
Mitochondrial localization of vitamin D receptor in human platelets and differentiated megakaryocytes. |
title_full_unstemmed |
Mitochondrial localization of vitamin D receptor in human platelets and differentiated megakaryocytes. |
title_sort |
mitochondrial localization of vitamin d receptor in human platelets and differentiated megakaryocytes. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2010-01-01 |
description |
BACKGROUND: Like other steroid hormones, vitamin D elicits both transcriptional events and rapid non genomic effects. Vitamin D receptor (VDR) localization and mechanisms of VDR-triggered non genomic responses are still controversial. Although anticoagulant effects of vitamin D have been reported and VDR signalling has been characterized in monocytes and vascular cells, nothing is known about VDR expression and functions in human platelets, anucleated fragments of megakaryocytes which are known targets of other steroids. METHODOLOGY/PRINCIPAL FINDINGS: In this study we characterized the expression and cellular localization of VDR in human platelets and in a megakaryocyte lineage. Human platelets and their TPA-differentiated precursors expressed a classical 50 kDa VDR protein, which increased with megakaryocytes maturation. By biochemical fractionation studies we demonstrated the presence of the receptor in the soluble and mitochondrial compartment of human platelets, and the observation was confirmed by immunoelectron microscopy analysis. Similar localization was found in mature megakaryocytes, where besides its classical nuclear localization the receptor was evident as soluble and mitochondria resident protein. CONCLUSIONS: The results reported here suggest that megakaryocytopoiesis and platelet activation, which are calcium-dependent events, might be modulated by a mitochondrial non genomic activity of VDR. These data open challenging future studies on VDR physiological role in platelets and more generally in mitochondria. |
url |
http://europepmc.org/articles/PMC2809087?pdf=render |
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