Assessment of Antioxidant and Cytotoxic Activity of Known Antioxidants Compared to Neopterin
Free radicals and other reactive oxygen species (ROS) are constantly formed in the human body. Free-radical mechanisms have been implicated in the pathology of several human diseases. Free radicals are generated physiologically during immune activation. Neopterin, an early marker of immune activatio...
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doaj-878a34c1dfa14c15ae15fd3ed393c5692020-11-24T23:54:56ZengMDPI AGProceedings2504-39002017-11-01110107110.3390/proceedings1101071proceedings1101071Assessment of Antioxidant and Cytotoxic Activity of Known Antioxidants Compared to NeopterinGözde Girgin0Suna Sabuncuoğlu1Ayşe Zeynep Ünal2Terken Baydar3Toxicology Department, Faculty of Pharmacy, Hacettepe University, Ankara 06000, TurkeyToxicology Department, Faculty of Pharmacy, Hacettepe University, Ankara 06000, TurkeyToxicology Department, Faculty of Pharmacy, Hacettepe University, Ankara 06000, TurkeyToxicology Department, Faculty of Pharmacy, Hacettepe University, Ankara 06000, TurkeyFree radicals and other reactive oxygen species (ROS) are constantly formed in the human body. Free-radical mechanisms have been implicated in the pathology of several human diseases. Free radicals are generated physiologically during immune activation. Neopterin, an early marker of immune activation is released from monocyte-macrophages with the induction of interferon-gamma. Its biological levels are known to be increased in different types of pathologies including cancer. Neopterin is also known to be related with oxidative stress. In this study, the antioxidant effect of neopterin and silymarin, melatonin and homocystein was evaluated by NO, SO, and DPPH radicals scavenging assays. Possible cytotoxic effects of the compounds were evaluated in 3 different cell lines by MTT and LDH assays. According to the results all the compounds have the radical scavenging activities. Therefore, none of them have a significant cytotoxic potential. However, only neopterin has a significant proliferative effect at very high concentrations. The results should be confirmed and reevaluated by in vivo and in vitro experiments.https://www.mdpi.com/2504-3900/1/10/1071neopterinsilymarinmelatoninhomocysteineantioxidantcytotoxicity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gözde Girgin Suna Sabuncuoğlu Ayşe Zeynep Ünal Terken Baydar |
spellingShingle |
Gözde Girgin Suna Sabuncuoğlu Ayşe Zeynep Ünal Terken Baydar Assessment of Antioxidant and Cytotoxic Activity of Known Antioxidants Compared to Neopterin Proceedings neopterin silymarin melatonin homocysteine antioxidant cytotoxicity |
author_facet |
Gözde Girgin Suna Sabuncuoğlu Ayşe Zeynep Ünal Terken Baydar |
author_sort |
Gözde Girgin |
title |
Assessment of Antioxidant and Cytotoxic Activity of Known Antioxidants Compared to Neopterin |
title_short |
Assessment of Antioxidant and Cytotoxic Activity of Known Antioxidants Compared to Neopterin |
title_full |
Assessment of Antioxidant and Cytotoxic Activity of Known Antioxidants Compared to Neopterin |
title_fullStr |
Assessment of Antioxidant and Cytotoxic Activity of Known Antioxidants Compared to Neopterin |
title_full_unstemmed |
Assessment of Antioxidant and Cytotoxic Activity of Known Antioxidants Compared to Neopterin |
title_sort |
assessment of antioxidant and cytotoxic activity of known antioxidants compared to neopterin |
publisher |
MDPI AG |
series |
Proceedings |
issn |
2504-3900 |
publishDate |
2017-11-01 |
description |
Free radicals and other reactive oxygen species (ROS) are constantly formed in the human body. Free-radical mechanisms have been implicated in the pathology of several human diseases. Free radicals are generated physiologically during immune activation. Neopterin, an early marker of immune activation is released from monocyte-macrophages with the induction of interferon-gamma. Its biological levels are known to be increased in different types of pathologies including cancer. Neopterin is also known to be related with oxidative stress. In this study, the antioxidant effect of neopterin and silymarin, melatonin and homocystein was evaluated by NO, SO, and DPPH radicals scavenging assays. Possible cytotoxic effects of the compounds were evaluated in 3 different cell lines by MTT and LDH assays. According to the results all the compounds have the radical scavenging activities. Therefore, none of them have a significant cytotoxic potential. However, only neopterin has a significant proliferative effect at very high concentrations. The results should be confirmed and reevaluated by in vivo and in vitro experiments. |
topic |
neopterin silymarin melatonin homocysteine antioxidant cytotoxicity |
url |
https://www.mdpi.com/2504-3900/1/10/1071 |
work_keys_str_mv |
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