Isolation and Characterization of Multipotent Cells from Human Fetal Dermis
We report that cells from human fetal dermis, termed here multipotent fetal dermal cells, can be isolated with high efficiency by using a nonenzymatic, cell outgrowth method. The resulting cell population was consistent with the definition of mesenchymal stromal cells by the International Society fo...
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Online Access: | https://doi.org/10.3727/096368913X668618 |
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doaj-878a6431ccc14b369f3a41c694ba406e2020-11-25T03:20:34ZengSAGE PublishingCell Transplantation0963-68971555-38922014-10-012310.3727/096368913X668618Isolation and Characterization of Multipotent Cells from Human Fetal DermisCinzia Maria Chinnici Ph.D0Giandomenico Amico1Marcello Monti2Stefania Motta3Rosario Casalone4Sergio Li Petri5Marco Spada6Bruno Gridelli7Pier Giulio Conaldi8Fondazione Ri.MED, Regenerative Medicine and Biomedical Technologies Unit, Department of Laboratory Medicine and Advanced Biotechnologies, ISMETT, Palermo, ItalyFondazione Ri.MED, Regenerative Medicine and Biomedical Technologies Unit, Department of Laboratory Medicine and Advanced Biotechnologies, ISMETT, Palermo, ItalyTranslational Medicine Department, University of Milan Istituto Clinico Humanitas, Milan, ItalyTranslational Medicine Department, University of Milan Istituto Clinico Humanitas, Milan, ItalySSD Genetica, Azienda Ospedaliera Ospedale di Circolo e Fondazione Macchi Polo, Varese, ItalyMediterranean Institute for Transplantation and Advanced Specialized Therapies (ISMETT), Palermo, ItalyMediterranean Institute for Transplantation and Advanced Specialized Therapies (ISMETT), Palermo, ItalyMediterranean Institute for Transplantation and Advanced Specialized Therapies (ISMETT), Palermo, ItalyMediterranean Institute for Transplantation and Advanced Specialized Therapies (ISMETT), Palermo, ItalyWe report that cells from human fetal dermis, termed here multipotent fetal dermal cells, can be isolated with high efficiency by using a nonenzymatic, cell outgrowth method. The resulting cell population was consistent with the definition of mesenchymal stromal cells by the International Society for Cellular Therapy. As multipotent fetal dermal cells proliferate extensively, with no loss of multilineage differentiation potential up to passage 25, they may be an ideal source for cell therapy to repair damaged tissues and organs. Multipotent fetal dermal cells were not recognized as targets by T lymphocytes in vitro, thus supporting their feasibility for allogenic transplantation. Moreover, the expansion protocol did not affect the normal phenotype and karyotype of cells. When compared with adult dermal cells, fetal cells displayed several advantages, including a greater cellular yield after isolation, the ability to proliferate longer, and the retention of differentiation potential. Interestingly, multipotent fetal dermal cells expressed the pluripotency marker SSEA4 (90.56 ± 3.15% fetal vs. 10.5 ± 8.5% adult) and coexpressed mesenchymal and epithelial markers (>80% CD90 + /CK18 + cells), coexpression lacking in the adult counterparts isolated under the same conditions. Multipotent fetal dermal cells were able to form capillary structures, as well as differentiate into a simple epithelium in vitro, indicating skin regeneration capabilities.https://doi.org/10.3727/096368913X668618 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Cinzia Maria Chinnici Ph.D Giandomenico Amico Marcello Monti Stefania Motta Rosario Casalone Sergio Li Petri Marco Spada Bruno Gridelli Pier Giulio Conaldi |
spellingShingle |
Cinzia Maria Chinnici Ph.D Giandomenico Amico Marcello Monti Stefania Motta Rosario Casalone Sergio Li Petri Marco Spada Bruno Gridelli Pier Giulio Conaldi Isolation and Characterization of Multipotent Cells from Human Fetal Dermis Cell Transplantation |
author_facet |
Cinzia Maria Chinnici Ph.D Giandomenico Amico Marcello Monti Stefania Motta Rosario Casalone Sergio Li Petri Marco Spada Bruno Gridelli Pier Giulio Conaldi |
author_sort |
Cinzia Maria Chinnici Ph.D |
title |
Isolation and Characterization of Multipotent Cells from Human Fetal Dermis |
title_short |
Isolation and Characterization of Multipotent Cells from Human Fetal Dermis |
title_full |
Isolation and Characterization of Multipotent Cells from Human Fetal Dermis |
title_fullStr |
Isolation and Characterization of Multipotent Cells from Human Fetal Dermis |
title_full_unstemmed |
Isolation and Characterization of Multipotent Cells from Human Fetal Dermis |
title_sort |
isolation and characterization of multipotent cells from human fetal dermis |
publisher |
SAGE Publishing |
series |
Cell Transplantation |
issn |
0963-6897 1555-3892 |
publishDate |
2014-10-01 |
description |
We report that cells from human fetal dermis, termed here multipotent fetal dermal cells, can be isolated with high efficiency by using a nonenzymatic, cell outgrowth method. The resulting cell population was consistent with the definition of mesenchymal stromal cells by the International Society for Cellular Therapy. As multipotent fetal dermal cells proliferate extensively, with no loss of multilineage differentiation potential up to passage 25, they may be an ideal source for cell therapy to repair damaged tissues and organs. Multipotent fetal dermal cells were not recognized as targets by T lymphocytes in vitro, thus supporting their feasibility for allogenic transplantation. Moreover, the expansion protocol did not affect the normal phenotype and karyotype of cells. When compared with adult dermal cells, fetal cells displayed several advantages, including a greater cellular yield after isolation, the ability to proliferate longer, and the retention of differentiation potential. Interestingly, multipotent fetal dermal cells expressed the pluripotency marker SSEA4 (90.56 ± 3.15% fetal vs. 10.5 ± 8.5% adult) and coexpressed mesenchymal and epithelial markers (>80% CD90 + /CK18 + cells), coexpression lacking in the adult counterparts isolated under the same conditions. Multipotent fetal dermal cells were able to form capillary structures, as well as differentiate into a simple epithelium in vitro, indicating skin regeneration capabilities. |
url |
https://doi.org/10.3727/096368913X668618 |
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