Immunogenicity of HLA-DR1 Restricted Peptides Derived from Leishmania major gp63 Using FVB/N-DR1 Transgenic Mouse Model

Immunogenicity of HLA-DR1 Restricted Peptides Derived from Leishmania major gp63 Using FVB/N-DR1 Transgenic Mouse Model

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  Background: Leishmaniasis is a worldwide disease prevalent in tropical and sub- tropical countries. In the present study the immunogenicity of three human HLA-DR1 restricted peptides derived from L. major gp63 protein was evaluated using FVB/N-DR1 transgenic mouse model. Methods: The immunit...

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Main Author: H Rezvan
Format: Article
Language:English
Published: Tehran University of Medical Sciences 2013-06-01
Series:Iranian Journal of Parasitology
Subjects:
FVB/N-DR1 transgenic mice
NIFN-γ
Leishmania major
Peptide
Proliferation
gp63
Online Access:https://ijpa.tums.ac.ir/index.php/ijpa/article/view/495
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spelling doaj-879666936c2742309c2e92022ef2fb2b2021-04-02T17:36:45ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2013-06-0182Immunogenicity of HLA-DR1 Restricted Peptides Derived from Leishmania major gp63 Using FVB/N-DR1 Transgenic Mouse ModelH Rezvan0Dept. of Laboratory Science, School of Paraveterinary Sciences, Bu-Ali Sina University, Hamadan, Iran.   Background: Leishmaniasis is a worldwide disease prevalent in tropical and sub- tropical countries. In the present study the immunogenicity of three human HLA-DR1 restricted peptides derived from L. major gp63 protein was evaluated using FVB/N-DR1 transgenic mouse model. Methods: The immunity generated by three MHC class II – restricted peptides with the sequence of AARLVRLAAAGAAVT (AAR), AAPLVRLAAAGAAVT (AAP) and SRYDQLVTRVVTHE (ASR) derived from L. major gp63 protein were predicted using a web-based software (SYFPEITHI) and tested in FVB/N-DR1 transgenic mice. Results: Immunization of FVB/N-DR1 transgenic mice with one of the three predicted peptides (AAR) resulted in high levels of Th1-type immune response as well as significant levels of IFN-γ de-tected by Proliferation assay and ELISA. Conclusion: The results indicate a high level of immunogenicity for AAR, which can be a potent candidate for peptide vaccine in Leishmania infections. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/495FVB/N-DR1 transgenic miceNIFN-γLeishmania majorPeptideProliferationgp63
collection DOAJ
language English
format Article
sources DOAJ
author H Rezvan
spellingShingle H Rezvan
Immunogenicity of HLA-DR1 Restricted Peptides Derived from Leishmania major gp63 Using FVB/N-DR1 Transgenic Mouse Model
Iranian Journal of Parasitology
FVB/N-DR1 transgenic mice
NIFN-γ
Leishmania major
Peptide
Proliferation
gp63
author_facet H Rezvan
author_sort H Rezvan
title Immunogenicity of HLA-DR1 Restricted Peptides Derived from Leishmania major gp63 Using FVB/N-DR1 Transgenic Mouse Model
title_short Immunogenicity of HLA-DR1 Restricted Peptides Derived from Leishmania major gp63 Using FVB/N-DR1 Transgenic Mouse Model
title_full Immunogenicity of HLA-DR1 Restricted Peptides Derived from Leishmania major gp63 Using FVB/N-DR1 Transgenic Mouse Model
title_fullStr Immunogenicity of HLA-DR1 Restricted Peptides Derived from Leishmania major gp63 Using FVB/N-DR1 Transgenic Mouse Model
title_full_unstemmed Immunogenicity of HLA-DR1 Restricted Peptides Derived from Leishmania major gp63 Using FVB/N-DR1 Transgenic Mouse Model
title_sort immunogenicity of hla-dr1 restricted peptides derived from leishmania major gp63 using fvb/n-dr1 transgenic mouse model
publisher Tehran University of Medical Sciences
series Iranian Journal of Parasitology
issn 1735-7020
2008-238X
publishDate 2013-06-01
description   Background: Leishmaniasis is a worldwide disease prevalent in tropical and sub- tropical countries. In the present study the immunogenicity of three human HLA-DR1 restricted peptides derived from L. major gp63 protein was evaluated using FVB/N-DR1 transgenic mouse model. Methods: The immunity generated by three MHC class II – restricted peptides with the sequence of AARLVRLAAAGAAVT (AAR), AAPLVRLAAAGAAVT (AAP) and SRYDQLVTRVVTHE (ASR) derived from L. major gp63 protein were predicted using a web-based software (SYFPEITHI) and tested in FVB/N-DR1 transgenic mice. Results: Immunization of FVB/N-DR1 transgenic mice with one of the three predicted peptides (AAR) resulted in high levels of Th1-type immune response as well as significant levels of IFN-γ de-tected by Proliferation assay and ELISA. Conclusion: The results indicate a high level of immunogenicity for AAR, which can be a potent candidate for peptide vaccine in Leishmania infections.
topic FVB/N-DR1 transgenic mice
NIFN-γ
Leishmania major
Peptide
Proliferation
gp63
url https://ijpa.tums.ac.ir/index.php/ijpa/article/view/495
work_keys_str_mv AT hrezvan immunogenicityofhladr1restrictedpeptidesderivedfromleishmaniamajorgp63usingfvbndr1transgenicmousemodel
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