Pan-Nematoda Transcriptomic Elucidation of Essential Intestinal Functions and Therapeutic Targets With Broad Potential
The nematode intestine is continuous with the outside environment, making it easily accessible to anthelmintics for parasite control, but the development of new therapeutics is impeded by limited knowledge of nematode intestinal cell biology. We established the most comprehensive nematode intestinal...
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doaj-87bc5392cf5a4607997265b518cad37c2020-11-25T02:36:19ZengElsevierEBioMedicine2352-39642015-09-01291079108910.1016/j.ebiom.2015.07.030Pan-Nematoda Transcriptomic Elucidation of Essential Intestinal Functions and Therapeutic Targets With Broad PotentialQi Wang0Bruce A. Rosa1Douglas P. Jasmer2Makedonka Mitreva3McDonnell Genome Institute, Washington University School of Medicine, St. Louis, MO, USAMcDonnell Genome Institute, Washington University School of Medicine, St. Louis, MO, USADepartment of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, USAMcDonnell Genome Institute, Washington University School of Medicine, St. Louis, MO, USAThe nematode intestine is continuous with the outside environment, making it easily accessible to anthelmintics for parasite control, but the development of new therapeutics is impeded by limited knowledge of nematode intestinal cell biology. We established the most comprehensive nematode intestinal functional database to date by generating transcriptional data from the dissected intestines of three parasitic nematodes spanning the phylum, and integrating the results with the whole proteomes of 10 nematodes (including 9 pathogens of humans or animals) and 3 host species and 2 outgroup species. We resolved 10,772 predicted nematode intestinal protein families (IntFams), and studied their presence and absence within the different lineages (births and deaths) among nematodes. Conserved intestinal cell functions representing ancestral functions of evolutionary importance were delineated, and molecular features useful for selective therapeutic targeting were identified. Molecular patterns conserved among IntFam proteins demonstrated large potential as therapeutic targets to inhibit intestinal cell functions with broad applications towards treatment and control of parasitic nematodes.http://www.sciencedirect.com/science/article/pii/S2352396415300827Nematode intestineMolecular functionsIntestinal protein familiesTherapeutic targets |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Qi Wang Bruce A. Rosa Douglas P. Jasmer Makedonka Mitreva |
spellingShingle |
Qi Wang Bruce A. Rosa Douglas P. Jasmer Makedonka Mitreva Pan-Nematoda Transcriptomic Elucidation of Essential Intestinal Functions and Therapeutic Targets With Broad Potential EBioMedicine Nematode intestine Molecular functions Intestinal protein families Therapeutic targets |
author_facet |
Qi Wang Bruce A. Rosa Douglas P. Jasmer Makedonka Mitreva |
author_sort |
Qi Wang |
title |
Pan-Nematoda Transcriptomic Elucidation of Essential Intestinal Functions and Therapeutic Targets With Broad Potential |
title_short |
Pan-Nematoda Transcriptomic Elucidation of Essential Intestinal Functions and Therapeutic Targets With Broad Potential |
title_full |
Pan-Nematoda Transcriptomic Elucidation of Essential Intestinal Functions and Therapeutic Targets With Broad Potential |
title_fullStr |
Pan-Nematoda Transcriptomic Elucidation of Essential Intestinal Functions and Therapeutic Targets With Broad Potential |
title_full_unstemmed |
Pan-Nematoda Transcriptomic Elucidation of Essential Intestinal Functions and Therapeutic Targets With Broad Potential |
title_sort |
pan-nematoda transcriptomic elucidation of essential intestinal functions and therapeutic targets with broad potential |
publisher |
Elsevier |
series |
EBioMedicine |
issn |
2352-3964 |
publishDate |
2015-09-01 |
description |
The nematode intestine is continuous with the outside environment, making it easily accessible to anthelmintics for parasite control, but the development of new therapeutics is impeded by limited knowledge of nematode intestinal cell biology. We established the most comprehensive nematode intestinal functional database to date by generating transcriptional data from the dissected intestines of three parasitic nematodes spanning the phylum, and integrating the results with the whole proteomes of 10 nematodes (including 9 pathogens of humans or animals) and 3 host species and 2 outgroup species. We resolved 10,772 predicted nematode intestinal protein families (IntFams), and studied their presence and absence within the different lineages (births and deaths) among nematodes. Conserved intestinal cell functions representing ancestral functions of evolutionary importance were delineated, and molecular features useful for selective therapeutic targeting were identified. Molecular patterns conserved among IntFam proteins demonstrated large potential as therapeutic targets to inhibit intestinal cell functions with broad applications towards treatment and control of parasitic nematodes. |
topic |
Nematode intestine Molecular functions Intestinal protein families Therapeutic targets |
url |
http://www.sciencedirect.com/science/article/pii/S2352396415300827 |
work_keys_str_mv |
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1724800716271255552 |