Different effects of granulocyte colony-stimulating factor and erythropoietin on erythropoiesis
Abstract Background Red blood cells are the most abundant cells in the blood that deliver oxygen to the whole body. Erythropoietin (EPO), a positive regulator of erythropoiesis, is currently the major treatment for chronic anemia. Granulocyte colony-stimulating factor (G-CSF) is a multifunctional cy...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2018-05-01
|
Series: | Stem Cell Research & Therapy |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s13287-018-0877-2 |
id |
doaj-87c1814be7bd4949824eb0936ab7ded0 |
---|---|
record_format |
Article |
spelling |
doaj-87c1814be7bd4949824eb0936ab7ded02020-11-25T00:18:33ZengBMCStem Cell Research & Therapy1757-65122018-05-01911910.1186/s13287-018-0877-2Different effects of granulocyte colony-stimulating factor and erythropoietin on erythropoiesisTzu-Lin Chen0Ya-Wen Chiang1Guan-Ling Lin2Hsin-Hou Chang3Te-Sheng Lien4Min-Hua Sheh5Der-Shan Sun6Department of Molecular Biology and Human Genetics, Tzu-Chi UniversityDepartment of Molecular Biology and Human Genetics, Tzu-Chi UniversityInstitute of Medical Sciences, Tzu-Chi UniversityDepartment of Molecular Biology and Human Genetics, Tzu-Chi UniversityDepartment of Molecular Biology and Human Genetics, Tzu-Chi UniversityDepartment of Molecular Biology and Human Genetics, Tzu-Chi UniversityDepartment of Molecular Biology and Human Genetics, Tzu-Chi UniversityAbstract Background Red blood cells are the most abundant cells in the blood that deliver oxygen to the whole body. Erythropoietin (EPO), a positive regulator of erythropoiesis, is currently the major treatment for chronic anemia. Granulocyte colony-stimulating factor (G-CSF) is a multifunctional cytokine and a well-known regulator of hematopoietic stem cell proliferation, differentiation, and mobilization. The use of EPO in combination with G-CSF has been reported to synergistically improve erythroid responses in a group of patients with myelodysplastic syndromes who did not respond to EPO treatment alone; however, the mechanism remains unclear. Methods C57BL/6 J mice injected with G-CSF or EPO were used to compare the erythropoiesis status and the efficiency of erythroid mobilization by flow cytometry. Results In this study, we found that G-CSF induced more orthochromatophilic erythroblast production than did EPO in the bone marrow and spleen. In addition, in contrast to EPO treatments, G-CSF treatments enhanced the efficiency of the mobilization of newly synthesized reticulocytes into peripheral blood. Our results demonstrated that the effects of G-CSF on erythropoiesis and erythrocytic mobilization were independent of EPO secretion and, in contrast to EPO, G-CSF promoted progression of erythropoiesis through transition of early stage R2 (basophilic erythroblasts) to late stage R4 (orthochromatophilic erythroblasts). Conclusions We demonstrate for the first time that G-CSF treatments induce a faster erythropoiesis-enhancing response than that of EPO. These findings suggest an alternative approach to treating acute anemia, especially when patients are experiencing a clinical emergency in remote areas without proper blood bank supplies.http://link.springer.com/article/10.1186/s13287-018-0877-2Granulocyte colony-stimulating factorErythropoietinErythropoiesisReticulocyteErythrocytic mobilization |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tzu-Lin Chen Ya-Wen Chiang Guan-Ling Lin Hsin-Hou Chang Te-Sheng Lien Min-Hua Sheh Der-Shan Sun |
spellingShingle |
Tzu-Lin Chen Ya-Wen Chiang Guan-Ling Lin Hsin-Hou Chang Te-Sheng Lien Min-Hua Sheh Der-Shan Sun Different effects of granulocyte colony-stimulating factor and erythropoietin on erythropoiesis Stem Cell Research & Therapy Granulocyte colony-stimulating factor Erythropoietin Erythropoiesis Reticulocyte Erythrocytic mobilization |
author_facet |
Tzu-Lin Chen Ya-Wen Chiang Guan-Ling Lin Hsin-Hou Chang Te-Sheng Lien Min-Hua Sheh Der-Shan Sun |
author_sort |
Tzu-Lin Chen |
title |
Different effects of granulocyte colony-stimulating factor and erythropoietin on erythropoiesis |
title_short |
Different effects of granulocyte colony-stimulating factor and erythropoietin on erythropoiesis |
title_full |
Different effects of granulocyte colony-stimulating factor and erythropoietin on erythropoiesis |
title_fullStr |
Different effects of granulocyte colony-stimulating factor and erythropoietin on erythropoiesis |
title_full_unstemmed |
Different effects of granulocyte colony-stimulating factor and erythropoietin on erythropoiesis |
title_sort |
different effects of granulocyte colony-stimulating factor and erythropoietin on erythropoiesis |
publisher |
BMC |
series |
Stem Cell Research & Therapy |
issn |
1757-6512 |
publishDate |
2018-05-01 |
description |
Abstract Background Red blood cells are the most abundant cells in the blood that deliver oxygen to the whole body. Erythropoietin (EPO), a positive regulator of erythropoiesis, is currently the major treatment for chronic anemia. Granulocyte colony-stimulating factor (G-CSF) is a multifunctional cytokine and a well-known regulator of hematopoietic stem cell proliferation, differentiation, and mobilization. The use of EPO in combination with G-CSF has been reported to synergistically improve erythroid responses in a group of patients with myelodysplastic syndromes who did not respond to EPO treatment alone; however, the mechanism remains unclear. Methods C57BL/6 J mice injected with G-CSF or EPO were used to compare the erythropoiesis status and the efficiency of erythroid mobilization by flow cytometry. Results In this study, we found that G-CSF induced more orthochromatophilic erythroblast production than did EPO in the bone marrow and spleen. In addition, in contrast to EPO treatments, G-CSF treatments enhanced the efficiency of the mobilization of newly synthesized reticulocytes into peripheral blood. Our results demonstrated that the effects of G-CSF on erythropoiesis and erythrocytic mobilization were independent of EPO secretion and, in contrast to EPO, G-CSF promoted progression of erythropoiesis through transition of early stage R2 (basophilic erythroblasts) to late stage R4 (orthochromatophilic erythroblasts). Conclusions We demonstrate for the first time that G-CSF treatments induce a faster erythropoiesis-enhancing response than that of EPO. These findings suggest an alternative approach to treating acute anemia, especially when patients are experiencing a clinical emergency in remote areas without proper blood bank supplies. |
topic |
Granulocyte colony-stimulating factor Erythropoietin Erythropoiesis Reticulocyte Erythrocytic mobilization |
url |
http://link.springer.com/article/10.1186/s13287-018-0877-2 |
work_keys_str_mv |
AT tzulinchen differenteffectsofgranulocytecolonystimulatingfactoranderythropoietinonerythropoiesis AT yawenchiang differenteffectsofgranulocytecolonystimulatingfactoranderythropoietinonerythropoiesis AT guanlinglin differenteffectsofgranulocytecolonystimulatingfactoranderythropoietinonerythropoiesis AT hsinhouchang differenteffectsofgranulocytecolonystimulatingfactoranderythropoietinonerythropoiesis AT teshenglien differenteffectsofgranulocytecolonystimulatingfactoranderythropoietinonerythropoiesis AT minhuasheh differenteffectsofgranulocytecolonystimulatingfactoranderythropoietinonerythropoiesis AT dershansun differenteffectsofgranulocytecolonystimulatingfactoranderythropoietinonerythropoiesis |
_version_ |
1725375888723279872 |