Psp140: an immunodominant antigen in the supernatant of Streptococcus pneumoniae culture
Background and Objectives: Streptococcus pneumoniae causes many lethal infections. Due to its reduced sensitivity to commonly used antibiotics, development of new strategies against pneumococcal infections seems to be necessary. We aimed to investigate immunodominant antigens in S. pneumoniae cultu...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Tehran University of Medical Sciences
2020-07-01
|
Series: | Iranian Journal of Microbiology |
Subjects: | |
Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/2512 |
id |
doaj-87e2643e3e924e01b1b58c94464a7952 |
---|---|
record_format |
Article |
spelling |
doaj-87e2643e3e924e01b1b58c94464a79522020-12-02T06:37:25ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472020-07-0112410.18502/ijm.v12i4.3938Psp140: an immunodominant antigen in the supernatant of Streptococcus pneumoniae cultureDavoud Afshar0Solmaz Ohadian Moghadam1Farhad Safarpoor Dehkordi2Reza Ranjbar3Amir Hasanzadeh4Department of Microbiology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, IranUro Oncology Research Center, Tehran University of Medical Sciences, Tehran, IranHalal Research Center of IRI, FDA, Tehran, IranMolecular Biology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, IranDepartment of Microbiology, Maragheh University of Medical Sciences, Maragheh, Iran Background and Objectives: Streptococcus pneumoniae causes many lethal infections. Due to its reduced sensitivity to commonly used antibiotics, development of new strategies against pneumococcal infections seems to be necessary. We aimed to investigate immunodominant antigens in S. pneumoniae culture supernatant in order to develop novel targets for pneumococcal vaccines. Materials and Methods: In this study S. pneumoniae ATCC49619 was sub-cultured into BHI broth from overnight culture at 37°C for 4 h. The supernatant proteins were precipitated using acetone precipitation method. A rabbit was intramuscularly immunized with alum adjuvant and 100 μg pneumococcal supernatant proteins, 6 times at 14 days' intervals to produce hyperimmune serum. ELISA assay was performed to determine the antibody level response to pneumococcal secretory proteins. Then dot blot applied for rapid evaluation of hyperimmune serum reactivity to pneumococcus supernatant proteins. The western blot was also used to determine the interaction of supernatant proteins with immunogenic rabbit's hyperimmune-serum. Results: According to the western blot analysis, the immunodominant protein had 140KDa molecular weight and designated as pneumococcal secretory protein140 (Psp140). Conclusion: The Psp140 protein in the supernatant of S. pneumoniae culture is an immunodominant protein and it is likely related to pneumococcal secretory protein or surface exposed protein which released into culture supernatant during bacterial growth. https://ijm.tums.ac.ir/index.php/ijm/article/view/2512Enzyme-linked immunosorbent assay;Immunodominant antigen;Streptococcus pneumoniae;Western blot |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Davoud Afshar Solmaz Ohadian Moghadam Farhad Safarpoor Dehkordi Reza Ranjbar Amir Hasanzadeh |
spellingShingle |
Davoud Afshar Solmaz Ohadian Moghadam Farhad Safarpoor Dehkordi Reza Ranjbar Amir Hasanzadeh Psp140: an immunodominant antigen in the supernatant of Streptococcus pneumoniae culture Iranian Journal of Microbiology Enzyme-linked immunosorbent assay; Immunodominant antigen; Streptococcus pneumoniae; Western blot |
author_facet |
Davoud Afshar Solmaz Ohadian Moghadam Farhad Safarpoor Dehkordi Reza Ranjbar Amir Hasanzadeh |
author_sort |
Davoud Afshar |
title |
Psp140: an immunodominant antigen in the supernatant of Streptococcus pneumoniae culture |
title_short |
Psp140: an immunodominant antigen in the supernatant of Streptococcus pneumoniae culture |
title_full |
Psp140: an immunodominant antigen in the supernatant of Streptococcus pneumoniae culture |
title_fullStr |
Psp140: an immunodominant antigen in the supernatant of Streptococcus pneumoniae culture |
title_full_unstemmed |
Psp140: an immunodominant antigen in the supernatant of Streptococcus pneumoniae culture |
title_sort |
psp140: an immunodominant antigen in the supernatant of streptococcus pneumoniae culture |
publisher |
Tehran University of Medical Sciences |
series |
Iranian Journal of Microbiology |
issn |
2008-3289 2008-4447 |
publishDate |
2020-07-01 |
description |
Background and Objectives: Streptococcus pneumoniae causes many lethal infections. Due to its reduced sensitivity to commonly used antibiotics, development of new strategies against pneumococcal infections seems to be necessary. We aimed to investigate immunodominant antigens in S. pneumoniae culture supernatant in order to develop novel targets for pneumococcal vaccines.
Materials and Methods: In this study S. pneumoniae ATCC49619 was sub-cultured into BHI broth from overnight culture at 37°C for 4 h. The supernatant proteins were precipitated using acetone precipitation method. A rabbit was intramuscularly immunized with alum adjuvant and 100 μg pneumococcal supernatant proteins, 6 times at 14 days' intervals to produce hyperimmune serum. ELISA assay was performed to determine the antibody level response to pneumococcal secretory proteins. Then dot blot applied for rapid evaluation of hyperimmune serum reactivity to pneumococcus supernatant proteins. The western blot was also used to determine the interaction of supernatant proteins with immunogenic rabbit's hyperimmune-serum.
Results: According to the western blot analysis, the immunodominant protein had 140KDa molecular weight and designated as pneumococcal secretory protein140 (Psp140).
Conclusion: The Psp140 protein in the supernatant of S. pneumoniae culture is an immunodominant protein and it is likely related to pneumococcal secretory protein or surface exposed protein which released into culture supernatant during bacterial growth.
|
topic |
Enzyme-linked immunosorbent assay; Immunodominant antigen; Streptococcus pneumoniae; Western blot |
url |
https://ijm.tums.ac.ir/index.php/ijm/article/view/2512 |
work_keys_str_mv |
AT davoudafshar psp140animmunodominantantigeninthesupernatantofstreptococcuspneumoniaeculture AT solmazohadianmoghadam psp140animmunodominantantigeninthesupernatantofstreptococcuspneumoniaeculture AT farhadsafarpoordehkordi psp140animmunodominantantigeninthesupernatantofstreptococcuspneumoniaeculture AT rezaranjbar psp140animmunodominantantigeninthesupernatantofstreptococcuspneumoniaeculture AT amirhasanzadeh psp140animmunodominantantigeninthesupernatantofstreptococcuspneumoniaeculture |
_version_ |
1724408584036417536 |