Intestinal Absorption Profile of Three Polygala Oligosaccharide Esters in Polygalae Radix and the Effects of Other Components in Polygalae Radix on Their Absorption

Oligosaccharide esters, which are among the main active components of Polygalae Radix (PR), demonstrate significant pharmacological activities in the human nervous system. In our previous research, some other constituents in PR were able to improve the bioavailability of oligosaccharide esters such...

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Main Authors: YinYing Ba, MengLin Wang, KunFeng Zhang, QiJun Chen, JiaJia Wang, Hang Lv, YanYan Jiang, Renbing Shi
Format: Article
Language:English
Published: Hindawi Limited 2019-01-01
Series:Evidence-Based Complementary and Alternative Medicine
Online Access:http://dx.doi.org/10.1155/2019/1379531
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spelling doaj-87fc718b3dd84d74a83722313e7528232020-11-25T01:20:31ZengHindawi LimitedEvidence-Based Complementary and Alternative Medicine1741-427X1741-42882019-01-01201910.1155/2019/13795311379531Intestinal Absorption Profile of Three Polygala Oligosaccharide Esters in Polygalae Radix and the Effects of Other Components in Polygalae Radix on Their AbsorptionYinYing Ba0MengLin Wang1KunFeng Zhang2QiJun Chen3JiaJia Wang4Hang Lv5YanYan Jiang6Renbing Shi7School of Traditional Chinese Medicine, Capital Medical University, No. 10, Toutiao, You-An-Men-Wai Street, Beijing 100069, ChinaSchool of Traditional Chinese Medicine, Capital Medical University, No. 10, Toutiao, You-An-Men-Wai Street, Beijing 100069, ChinaSchool of Traditional Chinese Medicine, Capital Medical University, No. 10, Toutiao, You-An-Men-Wai Street, Beijing 100069, ChinaSchool of Traditional Chinese Medicine, Capital Medical University, No. 10, Toutiao, You-An-Men-Wai Street, Beijing 100069, ChinaSchool of Traditional Chinese Medicine, Capital Medical University, No. 10, Toutiao, You-An-Men-Wai Street, Beijing 100069, ChinaSchool of Chinese Pharmacy, Beijing University of Chinese Medicine, No. 11, Beisanhuan Dong Street, Beijing 100013, ChinaSchool of Chinese Pharmacy, Beijing University of Chinese Medicine, No. 11, Beisanhuan Dong Street, Beijing 100013, ChinaSchool of Chinese Pharmacy, Beijing University of Chinese Medicine, No. 11, Beisanhuan Dong Street, Beijing 100013, ChinaOligosaccharide esters, which are among the main active components of Polygalae Radix (PR), demonstrate significant pharmacological activities in the human nervous system. In our previous research, some other constituents in PR were able to improve the bioavailability of oligosaccharide esters such as sibiricose A5 (SA5), sibiricose A6 (SA6), and 3,6′-disinapoyl sucrose (DISS), but the related components and their underlying mechanisms remain unknown. The present study aimed to investigate the intestinal absorptive profile of SA5, SA6, and DISS and the absorptive behavior influenced by the coadministration of polygalaxanthone III and total saponins of PR (TS) using an in vitro everted rat gut sac model, along with the possible mechanisms that may influence absorption. The results showed that TS could significantly enhance the absorption of SA5, SA6, and DISS monomers. Verapamil, a P-glycoprotein inhibitor, was able to elevate the absorption of SA5 and SA6, and an absorption experiment using Rho123 led us to conclude that TS influenced the absorption of SA5 and SA6 in a manner similar to that of a P-glycoprotein inhibitor. Sodium caprate, a paracellular absorption enhancer, was found to increase the absorption of SA5, SA6, and DISS. Results showed that the absorption mechanisms of SA5 and SA6 may combine active transport with paracellular passive penetration, while DISS’s absorption was dominated by paracellular passive penetration. However, the relationship between polygala saponins and the absorption of SA5, SA6, and DISS by paracellular passive penetration remain to be examined. This is the direction of our future research.http://dx.doi.org/10.1155/2019/1379531
collection DOAJ
language English
format Article
sources DOAJ
author YinYing Ba
MengLin Wang
KunFeng Zhang
QiJun Chen
JiaJia Wang
Hang Lv
YanYan Jiang
Renbing Shi
spellingShingle YinYing Ba
MengLin Wang
KunFeng Zhang
QiJun Chen
JiaJia Wang
Hang Lv
YanYan Jiang
Renbing Shi
Intestinal Absorption Profile of Three Polygala Oligosaccharide Esters in Polygalae Radix and the Effects of Other Components in Polygalae Radix on Their Absorption
Evidence-Based Complementary and Alternative Medicine
author_facet YinYing Ba
MengLin Wang
KunFeng Zhang
QiJun Chen
JiaJia Wang
Hang Lv
YanYan Jiang
Renbing Shi
author_sort YinYing Ba
title Intestinal Absorption Profile of Three Polygala Oligosaccharide Esters in Polygalae Radix and the Effects of Other Components in Polygalae Radix on Their Absorption
title_short Intestinal Absorption Profile of Three Polygala Oligosaccharide Esters in Polygalae Radix and the Effects of Other Components in Polygalae Radix on Their Absorption
title_full Intestinal Absorption Profile of Three Polygala Oligosaccharide Esters in Polygalae Radix and the Effects of Other Components in Polygalae Radix on Their Absorption
title_fullStr Intestinal Absorption Profile of Three Polygala Oligosaccharide Esters in Polygalae Radix and the Effects of Other Components in Polygalae Radix on Their Absorption
title_full_unstemmed Intestinal Absorption Profile of Three Polygala Oligosaccharide Esters in Polygalae Radix and the Effects of Other Components in Polygalae Radix on Their Absorption
title_sort intestinal absorption profile of three polygala oligosaccharide esters in polygalae radix and the effects of other components in polygalae radix on their absorption
publisher Hindawi Limited
series Evidence-Based Complementary and Alternative Medicine
issn 1741-427X
1741-4288
publishDate 2019-01-01
description Oligosaccharide esters, which are among the main active components of Polygalae Radix (PR), demonstrate significant pharmacological activities in the human nervous system. In our previous research, some other constituents in PR were able to improve the bioavailability of oligosaccharide esters such as sibiricose A5 (SA5), sibiricose A6 (SA6), and 3,6′-disinapoyl sucrose (DISS), but the related components and their underlying mechanisms remain unknown. The present study aimed to investigate the intestinal absorptive profile of SA5, SA6, and DISS and the absorptive behavior influenced by the coadministration of polygalaxanthone III and total saponins of PR (TS) using an in vitro everted rat gut sac model, along with the possible mechanisms that may influence absorption. The results showed that TS could significantly enhance the absorption of SA5, SA6, and DISS monomers. Verapamil, a P-glycoprotein inhibitor, was able to elevate the absorption of SA5 and SA6, and an absorption experiment using Rho123 led us to conclude that TS influenced the absorption of SA5 and SA6 in a manner similar to that of a P-glycoprotein inhibitor. Sodium caprate, a paracellular absorption enhancer, was found to increase the absorption of SA5, SA6, and DISS. Results showed that the absorption mechanisms of SA5 and SA6 may combine active transport with paracellular passive penetration, while DISS’s absorption was dominated by paracellular passive penetration. However, the relationship between polygala saponins and the absorption of SA5, SA6, and DISS by paracellular passive penetration remain to be examined. This is the direction of our future research.
url http://dx.doi.org/10.1155/2019/1379531
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