De novo biosynthesis of C-arabinosylated flavones by utilization of indica rice C-glycosyltransferases
Abstract Flavone C-arabinosides/xylosides are plant-originated glycoconjugates with various bioactivities. However, the potential utility of these molecules is hindered by their low abundance in nature. Engineering biosynthesis pathway in heterologous bacterial chassis provides a sustainable source...
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doaj-8816421d99934729b47a6a982e43f5372021-06-13T11:53:20ZengSpringerOpenBioresources and Bioprocessing2197-43652021-06-018111310.1186/s40643-021-00404-3De novo biosynthesis of C-arabinosylated flavones by utilization of indica rice C-glycosyltransferasesZhuo Chen0Yuwei Sun1Guangyi Wang2Ying Zhang3Qian Zhang4Yulian Zhang5Jianhua Li6Yong Wang7CAS-Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of SciencesCAS-Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of SciencesCAS-Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of SciencesCAS-Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of SciencesCAS-Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of SciencesCAS-Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of SciencesCAS-Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of SciencesCAS-Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of SciencesAbstract Flavone C-arabinosides/xylosides are plant-originated glycoconjugates with various bioactivities. However, the potential utility of these molecules is hindered by their low abundance in nature. Engineering biosynthesis pathway in heterologous bacterial chassis provides a sustainable source of these C-glycosides. We previously reported bifunctional C-glucosyl/C-arabinosyltransferases in Oryza sativa japonica and O. sativa indica, which influence the C-glycoside spectrum in different rice varieties. In this study, we proved the C-arabinosyl-transferring activity of rice C-glycosyltransferases (CGTs) on the mono-C-glucoside substrate nothofagin, followed by taking advantage of specific CGTs and introducing heterologous UDP-pentose supply, to realize the production of eight different C-arabinosides/xylosides in recombinant E. coli. Fed-batch fermentation and precursor supplement maximized the titer of rice-originated C-arabinosides to 20–110 mg/L in an E. coli chassis. The optimized final titer of schaftoside and apigenin di-C-arabinoside reached 19.87 and 113.16 mg/L, respectively. We demonstrate here the success of de novo bio-production of C-arabinosylated and C-xylosylated flavones by heterologous pathway reconstitution. These results lay a foundation for further optimal manufacture of complex flavonoid compounds in microbial cell factories.https://doi.org/10.1186/s40643-021-00404-3De novo biosynthesisC-Arabinoside flavoneC-GlycosyltransferaseRice |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhuo Chen Yuwei Sun Guangyi Wang Ying Zhang Qian Zhang Yulian Zhang Jianhua Li Yong Wang |
spellingShingle |
Zhuo Chen Yuwei Sun Guangyi Wang Ying Zhang Qian Zhang Yulian Zhang Jianhua Li Yong Wang De novo biosynthesis of C-arabinosylated flavones by utilization of indica rice C-glycosyltransferases Bioresources and Bioprocessing De novo biosynthesis C-Arabinoside flavone C-Glycosyltransferase Rice |
author_facet |
Zhuo Chen Yuwei Sun Guangyi Wang Ying Zhang Qian Zhang Yulian Zhang Jianhua Li Yong Wang |
author_sort |
Zhuo Chen |
title |
De novo biosynthesis of C-arabinosylated flavones by utilization of indica rice C-glycosyltransferases |
title_short |
De novo biosynthesis of C-arabinosylated flavones by utilization of indica rice C-glycosyltransferases |
title_full |
De novo biosynthesis of C-arabinosylated flavones by utilization of indica rice C-glycosyltransferases |
title_fullStr |
De novo biosynthesis of C-arabinosylated flavones by utilization of indica rice C-glycosyltransferases |
title_full_unstemmed |
De novo biosynthesis of C-arabinosylated flavones by utilization of indica rice C-glycosyltransferases |
title_sort |
de novo biosynthesis of c-arabinosylated flavones by utilization of indica rice c-glycosyltransferases |
publisher |
SpringerOpen |
series |
Bioresources and Bioprocessing |
issn |
2197-4365 |
publishDate |
2021-06-01 |
description |
Abstract Flavone C-arabinosides/xylosides are plant-originated glycoconjugates with various bioactivities. However, the potential utility of these molecules is hindered by their low abundance in nature. Engineering biosynthesis pathway in heterologous bacterial chassis provides a sustainable source of these C-glycosides. We previously reported bifunctional C-glucosyl/C-arabinosyltransferases in Oryza sativa japonica and O. sativa indica, which influence the C-glycoside spectrum in different rice varieties. In this study, we proved the C-arabinosyl-transferring activity of rice C-glycosyltransferases (CGTs) on the mono-C-glucoside substrate nothofagin, followed by taking advantage of specific CGTs and introducing heterologous UDP-pentose supply, to realize the production of eight different C-arabinosides/xylosides in recombinant E. coli. Fed-batch fermentation and precursor supplement maximized the titer of rice-originated C-arabinosides to 20–110 mg/L in an E. coli chassis. The optimized final titer of schaftoside and apigenin di-C-arabinoside reached 19.87 and 113.16 mg/L, respectively. We demonstrate here the success of de novo bio-production of C-arabinosylated and C-xylosylated flavones by heterologous pathway reconstitution. These results lay a foundation for further optimal manufacture of complex flavonoid compounds in microbial cell factories. |
topic |
De novo biosynthesis C-Arabinoside flavone C-Glycosyltransferase Rice |
url |
https://doi.org/10.1186/s40643-021-00404-3 |
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