A Label-Free Assay for Aminoacylation of tRNA

Aminoacylation of tRNA generates an aminoacyl-tRNA (aa-tRNA) that is active for protein synthesis on the ribosome. Quantification of aminoacylation of tRNA is critical to understand the mechanism of specificity and the flux of the aa-tRNA into the protein synthesis machinery, which determines the ra...

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Bibliographic Details
Main Authors: Howard Gamper, Ya-Ming Hou
Format: Article
Language:English
Published: MDPI AG 2020-10-01
Series:Genes
Subjects:
Online Access:https://www.mdpi.com/2073-4425/11/10/1173