Baculoviral inhibitor of apoptosis protein repeat-containing protein 3 delays early Wallerian degeneration after sciatic nerve injury
Wallerian degeneration is a complex biological process that occurs after nerve injury, and involves nerve degeneration and regeneration. Schwann cells play a crucial role in the cellular and molecular events of Wallerian degeneration of the peripheral nervous system. However, Wallerian degeneration...
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Wolters Kluwer Medknow Publications
2022-01-01
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doaj-88254b6683c448adb0908429c564bd632021-09-08T03:52:08ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53742022-01-0117484585310.4103/1673-5374.322474Baculoviral inhibitor of apoptosis protein repeat-containing protein 3 delays early Wallerian degeneration after sciatic nerve injuryMin CaiJian ShaoBryant YungYi WangNan-Nan GaoXi XuHuan-Huan ZhangYu-Mei FengDeng-Bing YaoWallerian degeneration is a complex biological process that occurs after nerve injury, and involves nerve degeneration and regeneration. Schwann cells play a crucial role in the cellular and molecular events of Wallerian degeneration of the peripheral nervous system. However, Wallerian degeneration regulating nerve injury and repair remains largely unknown, especially the early response. We have previously reported some key regulators of Wallerian degeneration after sciatic nerve injury. Baculoviral inhibitor of apoptosis protein repeat-containing protein 3 (BIRC3) is an important factor that regulates apoptosis-inhibiting protein. In this study, we established rat models of right sciatic nerve injury. In vitro Schwann cell models were also established and subjected to gene transfection to inhibit and overexpress BIRC3. The data indicated that BIRC3 expression was significantly up-regulated after sciatic nerve injury. Both BIRC3 upregulation and downregulation affected the migration, proliferation and apoptosis of Schwan cells and affected the expression of related factors through activating c-fos and ERK signal pathway. Inhibition of BIRC3 delayed early Wallerian degeneration through inhibiting the apoptosis of Schwann cells after sciatic nerve injury. These findings suggest that BIRC3 plays an important role in peripheral nerve injury repair and regeneration. The study was approved by the Institutional Animal Care and Use Committee of Nantong University, China (approval No. 2019-nsfc004) on March 1, 2019.http://www.nrronline.org/article.asp?issn=1673-5374;year=2022;volume=17;issue=4;spage=845;epage=853;aulast=Caiapoptosis; baculoviral inhibitor of apoptosis protein repeat-containing protein 3; nerve degeneration; rat; schwann cell; sciatic nerve injury; signal pathway; wallerian degeneration |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Min Cai Jian Shao Bryant Yung Yi Wang Nan-Nan Gao Xi Xu Huan-Huan Zhang Yu-Mei Feng Deng-Bing Yao |
spellingShingle |
Min Cai Jian Shao Bryant Yung Yi Wang Nan-Nan Gao Xi Xu Huan-Huan Zhang Yu-Mei Feng Deng-Bing Yao Baculoviral inhibitor of apoptosis protein repeat-containing protein 3 delays early Wallerian degeneration after sciatic nerve injury Neural Regeneration Research apoptosis; baculoviral inhibitor of apoptosis protein repeat-containing protein 3; nerve degeneration; rat; schwann cell; sciatic nerve injury; signal pathway; wallerian degeneration |
author_facet |
Min Cai Jian Shao Bryant Yung Yi Wang Nan-Nan Gao Xi Xu Huan-Huan Zhang Yu-Mei Feng Deng-Bing Yao |
author_sort |
Min Cai |
title |
Baculoviral inhibitor of apoptosis protein repeat-containing protein 3 delays early Wallerian degeneration after sciatic nerve injury |
title_short |
Baculoviral inhibitor of apoptosis protein repeat-containing protein 3 delays early Wallerian degeneration after sciatic nerve injury |
title_full |
Baculoviral inhibitor of apoptosis protein repeat-containing protein 3 delays early Wallerian degeneration after sciatic nerve injury |
title_fullStr |
Baculoviral inhibitor of apoptosis protein repeat-containing protein 3 delays early Wallerian degeneration after sciatic nerve injury |
title_full_unstemmed |
Baculoviral inhibitor of apoptosis protein repeat-containing protein 3 delays early Wallerian degeneration after sciatic nerve injury |
title_sort |
baculoviral inhibitor of apoptosis protein repeat-containing protein 3 delays early wallerian degeneration after sciatic nerve injury |
publisher |
Wolters Kluwer Medknow Publications |
series |
Neural Regeneration Research |
issn |
1673-5374 |
publishDate |
2022-01-01 |
description |
Wallerian degeneration is a complex biological process that occurs after nerve injury, and involves nerve degeneration and regeneration. Schwann cells play a crucial role in the cellular and molecular events of Wallerian degeneration of the peripheral nervous system. However, Wallerian degeneration regulating nerve injury and repair remains largely unknown, especially the early response. We have previously reported some key regulators of Wallerian degeneration after sciatic nerve injury. Baculoviral inhibitor of apoptosis protein repeat-containing protein 3 (BIRC3) is an important factor that regulates apoptosis-inhibiting protein. In this study, we established rat models of right sciatic nerve injury. In vitro Schwann cell models were also established and subjected to gene transfection to inhibit and overexpress BIRC3. The data indicated that BIRC3 expression was significantly up-regulated after sciatic nerve injury. Both BIRC3 upregulation and downregulation affected the migration, proliferation and apoptosis of Schwan cells and affected the expression of related factors through activating c-fos and ERK signal pathway. Inhibition of BIRC3 delayed early Wallerian degeneration through inhibiting the apoptosis of Schwann cells after sciatic nerve injury. These findings suggest that BIRC3 plays an important role in peripheral nerve injury repair and regeneration. The study was approved by the Institutional Animal Care and Use Committee of Nantong University, China (approval No. 2019-nsfc004) on March 1, 2019. |
topic |
apoptosis; baculoviral inhibitor of apoptosis protein repeat-containing protein 3; nerve degeneration; rat; schwann cell; sciatic nerve injury; signal pathway; wallerian degeneration |
url |
http://www.nrronline.org/article.asp?issn=1673-5374;year=2022;volume=17;issue=4;spage=845;epage=853;aulast=Cai |
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