Emergence of HIV-1 drug resistance mutations in mothers on treatment with a history of prophylaxis in Ghana
Abstract Background Antiretrovirals have been available in Ghana since 2003 for HIV-1 positive pregnant women for prevention of mother-to-child transmission (PMTCT). Suboptimal responses to treatment observed post-PMTCT interventions necessitated the need to investigate the profile of viral mutation...
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doaj-8835036d278e45d7a3cf3dc2990224f52020-11-24T21:55:12ZengBMCVirology Journal1743-422X2018-09-011511910.1186/s12985-018-1051-2Emergence of HIV-1 drug resistance mutations in mothers on treatment with a history of prophylaxis in GhanaAlexander Martin-Odoom0Charles Addoquaye Brown1John Kofi Odoom2Evelyn Yayra Bonney3Nana Afia Asante Ntim4Elena Delgado5Margaret Lartey6Kwamena William Sagoe7Theophilus Adiku8William Kwabena Ampofo9Department of Medical Laboratory Sciences, School of Biomedical & Allied Health Sciences, College of Health Sciences, University of GhanaDepartment of Medical Laboratory Sciences, School of Biomedical & Allied Health Sciences, College of Health Sciences, University of GhanaDepartment of Virology, Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of GhanaDepartment of Virology, Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of GhanaDepartment of Virology, Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of GhanaCentro Nacional de Microbiología, Department Patogenia Viral/Uni of Biology and Variability of HIV, Instituto de Salud Carlos IIISchool of Medicine and Dentistry, College of Health Sciences, University of GhanaDepartment of Medical Laboratory Sciences, School of Biomedical & Allied Health Sciences, College of Health Sciences, University of GhanaDepartment of Medical Laboratory Sciences, School of Biomedical & Allied Health Sciences, College of Health Sciences, University of GhanaDepartment of Virology, Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of GhanaAbstract Background Antiretrovirals have been available in Ghana since 2003 for HIV-1 positive pregnant women for prevention of mother-to-child transmission (PMTCT). Suboptimal responses to treatment observed post-PMTCT interventions necessitated the need to investigate the profile of viral mutations generated. This study investigated HIV-1 drug resistance profiles in mothers in selected centres in Ghana on treatment with a history of prophylaxis. Methods Genotypic Drug Resistance Testing for HIV-1 was carried out. Subtyping was done by phylogenetic analysis and Stanford HIV Database programme was used for drug resistance analysis and interpretation. To compare the significance between the different groups and the emergence of drug resistance mutations, p values were used. Results Participants who had prophylaxis before treatment, those who had treatment without prophylaxis and those yet to initiate PMTCT showed 32% (8), 5% (3) and 15% (4) HIV-1 drug resistance associated mutations respectively. The differences were significant with p value < 0.05. Resistance Associated Mutations (RAMs) were seen in 14 participants (35%) to nucleoside reverse transcriptase inhibitors (NRTIs) and non-nucleoside reverse transcriptase inhibitors (NNRTIs). The most common NRTI mutation found was M184 V; K103 N and A98G were the most common NNRTI mutations seen. Thymidine Analogue Mutations (TAMs) such as M41 L, K70R and T215Y were found in all the groups; the most common of the TAMs found were M41 L and T215Y. Majority of the subtypes were CRF02_AG (82%). Conclusion In Ghana initiation of uninterrupted treatment upon diagnosis, coupled with drug resistance testing, would produce a better treatment outcome for HIV-1 positive pregnant women.http://link.springer.com/article/10.1186/s12985-018-1051-2Antiretroviral therapyPhylogenetic analysisDrug resistance profilesTreatment outcome |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alexander Martin-Odoom Charles Addoquaye Brown John Kofi Odoom Evelyn Yayra Bonney Nana Afia Asante Ntim Elena Delgado Margaret Lartey Kwamena William Sagoe Theophilus Adiku William Kwabena Ampofo |
spellingShingle |
Alexander Martin-Odoom Charles Addoquaye Brown John Kofi Odoom Evelyn Yayra Bonney Nana Afia Asante Ntim Elena Delgado Margaret Lartey Kwamena William Sagoe Theophilus Adiku William Kwabena Ampofo Emergence of HIV-1 drug resistance mutations in mothers on treatment with a history of prophylaxis in Ghana Virology Journal Antiretroviral therapy Phylogenetic analysis Drug resistance profiles Treatment outcome |
author_facet |
Alexander Martin-Odoom Charles Addoquaye Brown John Kofi Odoom Evelyn Yayra Bonney Nana Afia Asante Ntim Elena Delgado Margaret Lartey Kwamena William Sagoe Theophilus Adiku William Kwabena Ampofo |
author_sort |
Alexander Martin-Odoom |
title |
Emergence of HIV-1 drug resistance mutations in mothers on treatment with a history of prophylaxis in Ghana |
title_short |
Emergence of HIV-1 drug resistance mutations in mothers on treatment with a history of prophylaxis in Ghana |
title_full |
Emergence of HIV-1 drug resistance mutations in mothers on treatment with a history of prophylaxis in Ghana |
title_fullStr |
Emergence of HIV-1 drug resistance mutations in mothers on treatment with a history of prophylaxis in Ghana |
title_full_unstemmed |
Emergence of HIV-1 drug resistance mutations in mothers on treatment with a history of prophylaxis in Ghana |
title_sort |
emergence of hiv-1 drug resistance mutations in mothers on treatment with a history of prophylaxis in ghana |
publisher |
BMC |
series |
Virology Journal |
issn |
1743-422X |
publishDate |
2018-09-01 |
description |
Abstract Background Antiretrovirals have been available in Ghana since 2003 for HIV-1 positive pregnant women for prevention of mother-to-child transmission (PMTCT). Suboptimal responses to treatment observed post-PMTCT interventions necessitated the need to investigate the profile of viral mutations generated. This study investigated HIV-1 drug resistance profiles in mothers in selected centres in Ghana on treatment with a history of prophylaxis. Methods Genotypic Drug Resistance Testing for HIV-1 was carried out. Subtyping was done by phylogenetic analysis and Stanford HIV Database programme was used for drug resistance analysis and interpretation. To compare the significance between the different groups and the emergence of drug resistance mutations, p values were used. Results Participants who had prophylaxis before treatment, those who had treatment without prophylaxis and those yet to initiate PMTCT showed 32% (8), 5% (3) and 15% (4) HIV-1 drug resistance associated mutations respectively. The differences were significant with p value < 0.05. Resistance Associated Mutations (RAMs) were seen in 14 participants (35%) to nucleoside reverse transcriptase inhibitors (NRTIs) and non-nucleoside reverse transcriptase inhibitors (NNRTIs). The most common NRTI mutation found was M184 V; K103 N and A98G were the most common NNRTI mutations seen. Thymidine Analogue Mutations (TAMs) such as M41 L, K70R and T215Y were found in all the groups; the most common of the TAMs found were M41 L and T215Y. Majority of the subtypes were CRF02_AG (82%). Conclusion In Ghana initiation of uninterrupted treatment upon diagnosis, coupled with drug resistance testing, would produce a better treatment outcome for HIV-1 positive pregnant women. |
topic |
Antiretroviral therapy Phylogenetic analysis Drug resistance profiles Treatment outcome |
url |
http://link.springer.com/article/10.1186/s12985-018-1051-2 |
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