Genomes of Fasciola hepatica from the Americas Reveal Colonization with Neorickettsia Endobacteria Related to the Agents of Potomac Horse and Human Sennetsu Fevers.
Food borne trematodes (FBTs) are an assemblage of platyhelminth parasites transmitted through the food chain, four of which are recognized as neglected tropical diseases (NTDs). Fascioliasis stands out among the other NTDs due to its broad and significant impact on both human and animal health, as F...
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doaj-883fbd5b5c5b4000bf2b0b4b8bee73752020-11-24T22:20:28ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042017-01-01131e100653710.1371/journal.pgen.1006537Genomes of Fasciola hepatica from the Americas Reveal Colonization with Neorickettsia Endobacteria Related to the Agents of Potomac Horse and Human Sennetsu Fevers.Samantha N McNultyJose F TortGabriel RinaldiKerstin FischerBruce A RosaPablo SmircichSantiago FontenlaYoung-Jun ChoiRahul TyagiKymberlie Hallsworth-PepinVictoria H MannLakshmi KammiliPatricia S LathamNicolas Dell'OcaFernanda DominguezCarlos CarmonaPeter U FischerPaul J BrindleyMakedonka MitrevaFood borne trematodes (FBTs) are an assemblage of platyhelminth parasites transmitted through the food chain, four of which are recognized as neglected tropical diseases (NTDs). Fascioliasis stands out among the other NTDs due to its broad and significant impact on both human and animal health, as Fasciola sp., are also considered major pathogens of domesticated ruminants. Here we present a reference genome sequence of the common liver fluke, Fasciola hepatica isolated from sheep, complementing previously reported isolate from cattle. A total of 14,642 genes were predicted from the 1.14 GB genome of the liver fluke. Comparative genomics indicated that F. hepatica Oregon and related food-borne trematodes are metabolically less constrained than schistosomes and cestodes, taking advantage of the richer millieux offered by the hepatobiliary organs. Protease families differentially expanded between diverse trematodes may facilitate migration and survival within the heterogeneous environments and niches within the mammalian host. Surprisingly, the sequencing of Oregon and Uruguay F. hepatica isolates led to the first discovery of an endobacteria in this species. Two contigs from the F. hepatica Oregon assembly were joined to complete the 859,205 bp genome of a novel Neorickettsia endobacterium (nFh) closely related to the etiological agents of human Sennetsu and Potomac horse fevers. Immunohistochemical studies targeting a Neorickettsia surface protein found nFh in specific organs and tissues of the adult trematode including the female reproductive tract, eggs, the Mehlis' gland, seminal vesicle, and oral suckers, suggesting putative routes for fluke-to-fluke and fluke-to-host transmission. The genomes of F. hepatica and nFh will serve as a resource for further exploration of the biology of F. hepatica, and specifically its newly discovered trans-kingdom interaction with nFh and the impact of both species on disease in ruminants and humans.http://europepmc.org/articles/PMC5257007?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Samantha N McNulty Jose F Tort Gabriel Rinaldi Kerstin Fischer Bruce A Rosa Pablo Smircich Santiago Fontenla Young-Jun Choi Rahul Tyagi Kymberlie Hallsworth-Pepin Victoria H Mann Lakshmi Kammili Patricia S Latham Nicolas Dell'Oca Fernanda Dominguez Carlos Carmona Peter U Fischer Paul J Brindley Makedonka Mitreva |
spellingShingle |
Samantha N McNulty Jose F Tort Gabriel Rinaldi Kerstin Fischer Bruce A Rosa Pablo Smircich Santiago Fontenla Young-Jun Choi Rahul Tyagi Kymberlie Hallsworth-Pepin Victoria H Mann Lakshmi Kammili Patricia S Latham Nicolas Dell'Oca Fernanda Dominguez Carlos Carmona Peter U Fischer Paul J Brindley Makedonka Mitreva Genomes of Fasciola hepatica from the Americas Reveal Colonization with Neorickettsia Endobacteria Related to the Agents of Potomac Horse and Human Sennetsu Fevers. PLoS Genetics |
author_facet |
Samantha N McNulty Jose F Tort Gabriel Rinaldi Kerstin Fischer Bruce A Rosa Pablo Smircich Santiago Fontenla Young-Jun Choi Rahul Tyagi Kymberlie Hallsworth-Pepin Victoria H Mann Lakshmi Kammili Patricia S Latham Nicolas Dell'Oca Fernanda Dominguez Carlos Carmona Peter U Fischer Paul J Brindley Makedonka Mitreva |
author_sort |
Samantha N McNulty |
title |
Genomes of Fasciola hepatica from the Americas Reveal Colonization with Neorickettsia Endobacteria Related to the Agents of Potomac Horse and Human Sennetsu Fevers. |
title_short |
Genomes of Fasciola hepatica from the Americas Reveal Colonization with Neorickettsia Endobacteria Related to the Agents of Potomac Horse and Human Sennetsu Fevers. |
title_full |
Genomes of Fasciola hepatica from the Americas Reveal Colonization with Neorickettsia Endobacteria Related to the Agents of Potomac Horse and Human Sennetsu Fevers. |
title_fullStr |
Genomes of Fasciola hepatica from the Americas Reveal Colonization with Neorickettsia Endobacteria Related to the Agents of Potomac Horse and Human Sennetsu Fevers. |
title_full_unstemmed |
Genomes of Fasciola hepatica from the Americas Reveal Colonization with Neorickettsia Endobacteria Related to the Agents of Potomac Horse and Human Sennetsu Fevers. |
title_sort |
genomes of fasciola hepatica from the americas reveal colonization with neorickettsia endobacteria related to the agents of potomac horse and human sennetsu fevers. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS Genetics |
issn |
1553-7390 1553-7404 |
publishDate |
2017-01-01 |
description |
Food borne trematodes (FBTs) are an assemblage of platyhelminth parasites transmitted through the food chain, four of which are recognized as neglected tropical diseases (NTDs). Fascioliasis stands out among the other NTDs due to its broad and significant impact on both human and animal health, as Fasciola sp., are also considered major pathogens of domesticated ruminants. Here we present a reference genome sequence of the common liver fluke, Fasciola hepatica isolated from sheep, complementing previously reported isolate from cattle. A total of 14,642 genes were predicted from the 1.14 GB genome of the liver fluke. Comparative genomics indicated that F. hepatica Oregon and related food-borne trematodes are metabolically less constrained than schistosomes and cestodes, taking advantage of the richer millieux offered by the hepatobiliary organs. Protease families differentially expanded between diverse trematodes may facilitate migration and survival within the heterogeneous environments and niches within the mammalian host. Surprisingly, the sequencing of Oregon and Uruguay F. hepatica isolates led to the first discovery of an endobacteria in this species. Two contigs from the F. hepatica Oregon assembly were joined to complete the 859,205 bp genome of a novel Neorickettsia endobacterium (nFh) closely related to the etiological agents of human Sennetsu and Potomac horse fevers. Immunohistochemical studies targeting a Neorickettsia surface protein found nFh in specific organs and tissues of the adult trematode including the female reproductive tract, eggs, the Mehlis' gland, seminal vesicle, and oral suckers, suggesting putative routes for fluke-to-fluke and fluke-to-host transmission. The genomes of F. hepatica and nFh will serve as a resource for further exploration of the biology of F. hepatica, and specifically its newly discovered trans-kingdom interaction with nFh and the impact of both species on disease in ruminants and humans. |
url |
http://europepmc.org/articles/PMC5257007?pdf=render |
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