Chemical composition and antimicrobial, antioxidant, and anti-inflammatory activities of Lepidium sativum seed oil
Lepidium sativum (garden cress) seed oil was examined for its antimicrobial, antioxidant, and anti-inflammatory activities. The oil was obtained by hydrodistillation, where gas chromatography coupled with mass spectrometry that utilized to study its chemical composition. Microdilution method was use...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2019-07-01
|
Series: | Saudi Journal of Biological Sciences |
Online Access: | http://www.sciencedirect.com/science/article/pii/S1319562X18301189 |
id |
doaj-88495ee8269b4f3a841518fe1d0d681c |
---|---|
record_format |
Article |
spelling |
doaj-88495ee8269b4f3a841518fe1d0d681c2020-11-25T01:12:17ZengElsevierSaudi Journal of Biological Sciences1319-562X2019-07-0126510891092Chemical composition and antimicrobial, antioxidant, and anti-inflammatory activities of Lepidium sativum seed oilFulwah Yahya Alqahtani0Fadilah Sfouq Aleanizy1Amany Z. Mahmoud2Nida Nayyar Farshori3Rihaf Alfaraj4Ebtesam Saad Al-sheddi5Ibrahim A. Alsarra6Department of Pharmaceutics, College of Pharmacy, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi Arabia; Corresponding author.Department of Pharmaceutics, College of Pharmacy, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi Arabia; Pharmaceutical Medicinal Chemistry Department, Faculty of Pharmacy, Assiut University, Assiut, EgyptPharmacognosy Department, College of Pharmacy, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi ArabiaPharmacognosy Department, College of Pharmacy, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmaceutics, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi ArabiaLepidium sativum (garden cress) seed oil was examined for its antimicrobial, antioxidant, and anti-inflammatory activities. The oil was obtained by hydrodistillation, where gas chromatography coupled with mass spectrometry that utilized to study its chemical composition. Microdilution method was used to test the antimicrobial effect of oil against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica, Klebsiella pneumoniae, and Candida albicans. The antioxidant activity was assessed by radical scavenging activity assay using 2,2-diphenyl-1-picrylhydrazyl radical. The major constituents found in the oil were 7,10-hexadecadienoic acid, 11-octadecenoic acid, 7,10,13-hexadecatrienoic acid, and behenic acid. The minimum inhibitory concentration (MIC) against all pathogens was 47.5 mg/ml, except for Salmonella enterica, which showed MIC of 90 mg/ml. The oil demonstrated antioxidant activity in a dose dependent pattern, with a half maximal inhibitory concentration (IC50) value of 40 mg/ml, and exerted anti-inflammatory activity, wherein 21% protection was shown at a concentration of 300 μg/ml. Thus, L. sativum seed oil shows antimicrobial, antioxidant, and anti-inflammatory properties. Keywords: Lepidium sativum oil, Chemical composition, Antioxidant activity, Anti-inflammatory activity, Antimicrobial activityhttp://www.sciencedirect.com/science/article/pii/S1319562X18301189 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fulwah Yahya Alqahtani Fadilah Sfouq Aleanizy Amany Z. Mahmoud Nida Nayyar Farshori Rihaf Alfaraj Ebtesam Saad Al-sheddi Ibrahim A. Alsarra |
spellingShingle |
Fulwah Yahya Alqahtani Fadilah Sfouq Aleanizy Amany Z. Mahmoud Nida Nayyar Farshori Rihaf Alfaraj Ebtesam Saad Al-sheddi Ibrahim A. Alsarra Chemical composition and antimicrobial, antioxidant, and anti-inflammatory activities of Lepidium sativum seed oil Saudi Journal of Biological Sciences |
author_facet |
Fulwah Yahya Alqahtani Fadilah Sfouq Aleanizy Amany Z. Mahmoud Nida Nayyar Farshori Rihaf Alfaraj Ebtesam Saad Al-sheddi Ibrahim A. Alsarra |
author_sort |
Fulwah Yahya Alqahtani |
title |
Chemical composition and antimicrobial, antioxidant, and anti-inflammatory activities of Lepidium sativum seed oil |
title_short |
Chemical composition and antimicrobial, antioxidant, and anti-inflammatory activities of Lepidium sativum seed oil |
title_full |
Chemical composition and antimicrobial, antioxidant, and anti-inflammatory activities of Lepidium sativum seed oil |
title_fullStr |
Chemical composition and antimicrobial, antioxidant, and anti-inflammatory activities of Lepidium sativum seed oil |
title_full_unstemmed |
Chemical composition and antimicrobial, antioxidant, and anti-inflammatory activities of Lepidium sativum seed oil |
title_sort |
chemical composition and antimicrobial, antioxidant, and anti-inflammatory activities of lepidium sativum seed oil |
publisher |
Elsevier |
series |
Saudi Journal of Biological Sciences |
issn |
1319-562X |
publishDate |
2019-07-01 |
description |
Lepidium sativum (garden cress) seed oil was examined for its antimicrobial, antioxidant, and anti-inflammatory activities. The oil was obtained by hydrodistillation, where gas chromatography coupled with mass spectrometry that utilized to study its chemical composition. Microdilution method was used to test the antimicrobial effect of oil against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica, Klebsiella pneumoniae, and Candida albicans. The antioxidant activity was assessed by radical scavenging activity assay using 2,2-diphenyl-1-picrylhydrazyl radical. The major constituents found in the oil were 7,10-hexadecadienoic acid, 11-octadecenoic acid, 7,10,13-hexadecatrienoic acid, and behenic acid. The minimum inhibitory concentration (MIC) against all pathogens was 47.5 mg/ml, except for Salmonella enterica, which showed MIC of 90 mg/ml. The oil demonstrated antioxidant activity in a dose dependent pattern, with a half maximal inhibitory concentration (IC50) value of 40 mg/ml, and exerted anti-inflammatory activity, wherein 21% protection was shown at a concentration of 300 μg/ml. Thus, L. sativum seed oil shows antimicrobial, antioxidant, and anti-inflammatory properties. Keywords: Lepidium sativum oil, Chemical composition, Antioxidant activity, Anti-inflammatory activity, Antimicrobial activity |
url |
http://www.sciencedirect.com/science/article/pii/S1319562X18301189 |
work_keys_str_mv |
AT fulwahyahyaalqahtani chemicalcompositionandantimicrobialantioxidantandantiinflammatoryactivitiesoflepidiumsativumseedoil AT fadilahsfouqaleanizy chemicalcompositionandantimicrobialantioxidantandantiinflammatoryactivitiesoflepidiumsativumseedoil AT amanyzmahmoud chemicalcompositionandantimicrobialantioxidantandantiinflammatoryactivitiesoflepidiumsativumseedoil AT nidanayyarfarshori chemicalcompositionandantimicrobialantioxidantandantiinflammatoryactivitiesoflepidiumsativumseedoil AT rihafalfaraj chemicalcompositionandantimicrobialantioxidantandantiinflammatoryactivitiesoflepidiumsativumseedoil AT ebtesamsaadalsheddi chemicalcompositionandantimicrobialantioxidantandantiinflammatoryactivitiesoflepidiumsativumseedoil AT ibrahimaalsarra chemicalcompositionandantimicrobialantioxidantandantiinflammatoryactivitiesoflepidiumsativumseedoil |
_version_ |
1725167264435535872 |