Characterization of cellulase secretion and Cre1-mediated carbon source repression in the potential lignocellulose-degrading strain Trichoderma asperellum T-1.
Trichoderma asperellum, a traditional bio-control species, was demonstrated to be an excellent candidate for lignocellulose degradation in this work. Comparing to the representatively industrial strain of Trichoderma reeseiQM6a, T. asperellum T-1 showed more robust growth, stronger spore production,...
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doaj-88657c81b3d94bf99da06ff3155f468e2021-03-03T20:09:24ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01103e011923710.1371/journal.pone.0119237Characterization of cellulase secretion and Cre1-mediated carbon source repression in the potential lignocellulose-degrading strain Trichoderma asperellum T-1.Qun WangHui LinQi ShenXiaoping FanNaling BaiYuhua ZhaoTrichoderma asperellum, a traditional bio-control species, was demonstrated to be an excellent candidate for lignocellulose degradation in this work. Comparing to the representatively industrial strain of Trichoderma reeseiQM6a, T. asperellum T-1 showed more robust growth, stronger spore production, faster secretion of lignocellulose-decomposing enzymes and better pH tolerance. The reducing sugar released by strain T-1 on the second day of fermentation was 87% higher than that of strain QM6a, although the maximum reducing sugar yield and the cellulase production persistence of the strain T-1 were lower. Our experiment found that the cellulase secretion was strongly inhibited by glucose, suggesting the existence of carbon source repression pathway in T. asperellum T-1. The inhibiting effect was enhanced with an increase in glucose concentration and was closely related to mycelium growth. SDS-PAGE and secondary mass-spectrum identification confirmed that the expression of endo-1,4-β-xylanase I in T. asperellum T-1 was down-regulated when glucose was added. The factor Cre1, which plays an important role in the down-regulation of the endo-1,4-β-xylanase I gene, was investigated by bioinformatics methods. The protein structure of Cre1, analyzed using multiple protein sequence alignment, indicates the existence of the Zn-fingers domain. Then, the binding sites of Cre1 on the endo-1,4-β-xylanase I gene promoter were further elucidated. This study is the first report about Cre1-mediated carbon repression in the bio-control strain T. asperellum T-1. All of the above results provided good references for better understanding T. asperellum T-1 and improving its application for lignocellulose degradation.https://doi.org/10.1371/journal.pone.0119237 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Qun Wang Hui Lin Qi Shen Xiaoping Fan Naling Bai Yuhua Zhao |
spellingShingle |
Qun Wang Hui Lin Qi Shen Xiaoping Fan Naling Bai Yuhua Zhao Characterization of cellulase secretion and Cre1-mediated carbon source repression in the potential lignocellulose-degrading strain Trichoderma asperellum T-1. PLoS ONE |
author_facet |
Qun Wang Hui Lin Qi Shen Xiaoping Fan Naling Bai Yuhua Zhao |
author_sort |
Qun Wang |
title |
Characterization of cellulase secretion and Cre1-mediated carbon source repression in the potential lignocellulose-degrading strain Trichoderma asperellum T-1. |
title_short |
Characterization of cellulase secretion and Cre1-mediated carbon source repression in the potential lignocellulose-degrading strain Trichoderma asperellum T-1. |
title_full |
Characterization of cellulase secretion and Cre1-mediated carbon source repression in the potential lignocellulose-degrading strain Trichoderma asperellum T-1. |
title_fullStr |
Characterization of cellulase secretion and Cre1-mediated carbon source repression in the potential lignocellulose-degrading strain Trichoderma asperellum T-1. |
title_full_unstemmed |
Characterization of cellulase secretion and Cre1-mediated carbon source repression in the potential lignocellulose-degrading strain Trichoderma asperellum T-1. |
title_sort |
characterization of cellulase secretion and cre1-mediated carbon source repression in the potential lignocellulose-degrading strain trichoderma asperellum t-1. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2015-01-01 |
description |
Trichoderma asperellum, a traditional bio-control species, was demonstrated to be an excellent candidate for lignocellulose degradation in this work. Comparing to the representatively industrial strain of Trichoderma reeseiQM6a, T. asperellum T-1 showed more robust growth, stronger spore production, faster secretion of lignocellulose-decomposing enzymes and better pH tolerance. The reducing sugar released by strain T-1 on the second day of fermentation was 87% higher than that of strain QM6a, although the maximum reducing sugar yield and the cellulase production persistence of the strain T-1 were lower. Our experiment found that the cellulase secretion was strongly inhibited by glucose, suggesting the existence of carbon source repression pathway in T. asperellum T-1. The inhibiting effect was enhanced with an increase in glucose concentration and was closely related to mycelium growth. SDS-PAGE and secondary mass-spectrum identification confirmed that the expression of endo-1,4-β-xylanase I in T. asperellum T-1 was down-regulated when glucose was added. The factor Cre1, which plays an important role in the down-regulation of the endo-1,4-β-xylanase I gene, was investigated by bioinformatics methods. The protein structure of Cre1, analyzed using multiple protein sequence alignment, indicates the existence of the Zn-fingers domain. Then, the binding sites of Cre1 on the endo-1,4-β-xylanase I gene promoter were further elucidated. This study is the first report about Cre1-mediated carbon repression in the bio-control strain T. asperellum T-1. All of the above results provided good references for better understanding T. asperellum T-1 and improving its application for lignocellulose degradation. |
url |
https://doi.org/10.1371/journal.pone.0119237 |
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