Comparative Genome-Wide-Association Mapping Identifies Common Loci Controlling Root System Architecture and Resistance to Aphanomyces euteiches in Pea
Combining plant genetic resistance with architectural traits that are unfavorable to disease development is a promising strategy for reducing epidemics. However, few studies have identified root system architecture (RSA) traits with the potential to limit root disease development. Pea is a major cul...
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Frontiers Media S.A.
2018-01-01
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Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2017.02195/full |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Aurore Desgroux Aurore Desgroux Aurore Desgroux Valentin N. Baudais Véronique Aubert Gwenola Le Roy Gwenola Le Roy Henri de Larambergue Henri Miteul Henri Miteul Grégoire Aubert Gilles Boutet Gilles Boutet Gérard Duc Alain Baranger Alain Baranger Judith Burstin Maria Manzanares-Dauleux Marie-Laure Pilet-Nayel Marie-Laure Pilet-Nayel Virginie Bourion |
spellingShingle |
Aurore Desgroux Aurore Desgroux Aurore Desgroux Valentin N. Baudais Véronique Aubert Gwenola Le Roy Gwenola Le Roy Henri de Larambergue Henri Miteul Henri Miteul Grégoire Aubert Gilles Boutet Gilles Boutet Gérard Duc Alain Baranger Alain Baranger Judith Burstin Maria Manzanares-Dauleux Marie-Laure Pilet-Nayel Marie-Laure Pilet-Nayel Virginie Bourion Comparative Genome-Wide-Association Mapping Identifies Common Loci Controlling Root System Architecture and Resistance to Aphanomyces euteiches in Pea Frontiers in Plant Science root system architecture disease resistance Pisum sativum GWAS root rot |
author_facet |
Aurore Desgroux Aurore Desgroux Aurore Desgroux Valentin N. Baudais Véronique Aubert Gwenola Le Roy Gwenola Le Roy Henri de Larambergue Henri Miteul Henri Miteul Grégoire Aubert Gilles Boutet Gilles Boutet Gérard Duc Alain Baranger Alain Baranger Judith Burstin Maria Manzanares-Dauleux Marie-Laure Pilet-Nayel Marie-Laure Pilet-Nayel Virginie Bourion |
author_sort |
Aurore Desgroux |
title |
Comparative Genome-Wide-Association Mapping Identifies Common Loci Controlling Root System Architecture and Resistance to Aphanomyces euteiches in Pea |
title_short |
Comparative Genome-Wide-Association Mapping Identifies Common Loci Controlling Root System Architecture and Resistance to Aphanomyces euteiches in Pea |
title_full |
Comparative Genome-Wide-Association Mapping Identifies Common Loci Controlling Root System Architecture and Resistance to Aphanomyces euteiches in Pea |
title_fullStr |
Comparative Genome-Wide-Association Mapping Identifies Common Loci Controlling Root System Architecture and Resistance to Aphanomyces euteiches in Pea |
title_full_unstemmed |
Comparative Genome-Wide-Association Mapping Identifies Common Loci Controlling Root System Architecture and Resistance to Aphanomyces euteiches in Pea |
title_sort |
comparative genome-wide-association mapping identifies common loci controlling root system architecture and resistance to aphanomyces euteiches in pea |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2018-01-01 |
description |
Combining plant genetic resistance with architectural traits that are unfavorable to disease development is a promising strategy for reducing epidemics. However, few studies have identified root system architecture (RSA) traits with the potential to limit root disease development. Pea is a major cultivated legume worldwide and has a wide level of natural genetic variability for plant architecture. The root pathogen Aphanomyces euteiches is a major limiting factor of pea crop yield. This study aimed to increase the knowledge on the diversity of loci and candidate genes controlling RSA traits in pea and identify RSA genetic loci associated with resistance to A. euteiches which could be combined with resistance QTL in breeding. A comparative genome wide association (GWA) study of plant architecture and resistance to A. euteiches was conducted at the young plant stage in a collection of 266 pea lines contrasted for both traits. The collection was genotyped using 14,157 SNP markers from recent pea genomic resources. It was phenotyped for ten root, shoot and overall plant architecture traits, as well as three disease resistance traits in controlled conditions, using image analysis. We identified a total of 75 short-size genomic intervals significantly associated with plant architecture and overlapping with 46 previously detected QTL. The major consistent intervals included plant shoot architecture or flowering genes (PsLE, PsTFL1) with putative pleiotropic effects on root architecture. A total of 11 genomic intervals were significantly associated with resistance to A. euteiches confirming several consistent previously identified major QTL. One significant SNP, mapped to the major QTL Ae-Ps7.6, was associated with both resistance and RSA traits. At this marker, the resistance-enhancing allele was associated with an increased total root projected area, in accordance with the correlation observed between resistance and larger root systems in the collection. Seven additional intervals associated with plant architecture overlapped with GWA intervals previously identified for resistance to A. euteiches. This study provides innovative results about genetic interdependency of root disease resistance and RSA inheritance. It identifies pea lines, QTL, closely-linked markers and candidate genes for marker-assisted-selection of RSA loci to reduce Aphanomyces root rot severity in future pea varieties. |
topic |
root system architecture disease resistance Pisum sativum GWAS root rot |
url |
http://journal.frontiersin.org/article/10.3389/fpls.2017.02195/full |
work_keys_str_mv |
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doaj-8876e8cd50004531b68bbea9d321c4e82020-11-24T21:45:56ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2018-01-01810.3389/fpls.2017.02195315629Comparative Genome-Wide-Association Mapping Identifies Common Loci Controlling Root System Architecture and Resistance to Aphanomyces euteiches in PeaAurore Desgroux0Aurore Desgroux1Aurore Desgroux2Valentin N. Baudais3Véronique Aubert4Gwenola Le Roy5Gwenola Le Roy6Henri de Larambergue7Henri Miteul8Henri Miteul9Grégoire Aubert10Gilles Boutet11Gilles Boutet12Gérard Duc13Alain Baranger14Alain Baranger15Judith Burstin16Maria Manzanares-Dauleux17Marie-Laure Pilet-Nayel18Marie-Laure Pilet-Nayel19Virginie Bourion20Institut de Génétique, Environnement et Protection des Plantes, INRA, Agrocampus Ouest, Université Rennes 1, Le Rheu, FranceAgroécologie, INRA, AgroSup Dijon, Université Bourgogne Franche-Comté, Dijon, FrancePISOM, UMT INRA/Terre Inovia, Le Rheu, FranceInstitut de Génétique, Environnement et Protection des Plantes, INRA, Agrocampus Ouest, Université Rennes 1, Le Rheu, FranceAgroécologie, INRA, AgroSup Dijon, Université Bourgogne Franche-Comté, Dijon, FranceInstitut de Génétique, Environnement et Protection des Plantes, INRA, Agrocampus Ouest, Université Rennes 1, Le Rheu, FrancePISOM, UMT INRA/Terre Inovia, Le Rheu, FranceAgroécologie, INRA, AgroSup Dijon, Université Bourgogne Franche-Comté, Dijon, FranceInstitut de Génétique, Environnement et Protection des Plantes, INRA, Agrocampus Ouest, Université Rennes 1, Le Rheu, FrancePISOM, UMT INRA/Terre Inovia, Le Rheu, FranceAgroécologie, INRA, AgroSup Dijon, Université Bourgogne Franche-Comté, Dijon, FranceInstitut de Génétique, Environnement et Protection des Plantes, INRA, Agrocampus Ouest, Université Rennes 1, Le Rheu, FrancePISOM, UMT INRA/Terre Inovia, Le Rheu, FranceAgroécologie, INRA, AgroSup Dijon, Université Bourgogne Franche-Comté, Dijon, FranceInstitut de Génétique, Environnement et Protection des Plantes, INRA, Agrocampus Ouest, Université Rennes 1, Le Rheu, FrancePISOM, UMT INRA/Terre Inovia, Le Rheu, FranceAgroécologie, INRA, AgroSup Dijon, Université Bourgogne Franche-Comté, Dijon, FranceInstitut de Génétique, Environnement et Protection des Plantes, INRA, Agrocampus Ouest, Université Rennes 1, Le Rheu, FranceInstitut de Génétique, Environnement et Protection des Plantes, INRA, Agrocampus Ouest, Université Rennes 1, Le Rheu, FrancePISOM, UMT INRA/Terre Inovia, Le Rheu, FranceAgroécologie, INRA, AgroSup Dijon, Université Bourgogne Franche-Comté, Dijon, FranceCombining plant genetic resistance with architectural traits that are unfavorable to disease development is a promising strategy for reducing epidemics. However, few studies have identified root system architecture (RSA) traits with the potential to limit root disease development. Pea is a major cultivated legume worldwide and has a wide level of natural genetic variability for plant architecture. The root pathogen Aphanomyces euteiches is a major limiting factor of pea crop yield. This study aimed to increase the knowledge on the diversity of loci and candidate genes controlling RSA traits in pea and identify RSA genetic loci associated with resistance to A. euteiches which could be combined with resistance QTL in breeding. A comparative genome wide association (GWA) study of plant architecture and resistance to A. euteiches was conducted at the young plant stage in a collection of 266 pea lines contrasted for both traits. The collection was genotyped using 14,157 SNP markers from recent pea genomic resources. It was phenotyped for ten root, shoot and overall plant architecture traits, as well as three disease resistance traits in controlled conditions, using image analysis. We identified a total of 75 short-size genomic intervals significantly associated with plant architecture and overlapping with 46 previously detected QTL. The major consistent intervals included plant shoot architecture or flowering genes (PsLE, PsTFL1) with putative pleiotropic effects on root architecture. A total of 11 genomic intervals were significantly associated with resistance to A. euteiches confirming several consistent previously identified major QTL. One significant SNP, mapped to the major QTL Ae-Ps7.6, was associated with both resistance and RSA traits. At this marker, the resistance-enhancing allele was associated with an increased total root projected area, in accordance with the correlation observed between resistance and larger root systems in the collection. Seven additional intervals associated with plant architecture overlapped with GWA intervals previously identified for resistance to A. euteiches. This study provides innovative results about genetic interdependency of root disease resistance and RSA inheritance. It identifies pea lines, QTL, closely-linked markers and candidate genes for marker-assisted-selection of RSA loci to reduce Aphanomyces root rot severity in future pea varieties.http://journal.frontiersin.org/article/10.3389/fpls.2017.02195/fullroot system architecturedisease resistancePisum sativumGWASroot rot |