Multi-functional roles of TaSSI2 involved in Fusarium head blight and powdery mildew resistance and drought tolerance
The mutation of the gene encoding a stearoyl-acyl carrier protein fatty acid desaturase (ssi2) has been proved to enhance pathogen resistance in several plants, while it's potential to regulate biotic and abiotic stresses in wheat is still unclear. In this study, we cloned TaSSI2 gene in wheat...
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doaj-888df4aeb7c14a41a45723d6cd1067cf2021-06-08T04:38:50ZengElsevierJournal of Integrative Agriculture2095-31192018-02-01172368380Multi-functional roles of TaSSI2 involved in Fusarium head blight and powdery mildew resistance and drought toleranceLi-qin HU0Jing-jing MU1Pei-sen SU2Hong-yan WU3Guang-hui YU4Gui-ping WANG5Liang WANG6Xin MA7An-fei LI8Hong-wei WANG9Lan-fei ZHAO10Ling-rang KONG11State Key Laboratory of Crop Biology/Shandong Key Laboratory of Crop Biology/College of Agronomy, Shandong Agricultural University, Tai'an 271018, P.R. ChinaAgriculture Bureau of Rushan, Rushan 264500, P.R. ChinaState Key Laboratory of Crop Biology/Shandong Key Laboratory of Crop Biology/College of Agronomy, Shandong Agricultural University, Tai'an 271018, P.R. ChinaShandong Agricultural University Fertilizer Science Technology Co., Ltd., Feicheng 271600, P.R. ChinaCollege of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, P.R. ChinaState Key Laboratory of Crop Biology/Shandong Key Laboratory of Crop Biology/College of Agronomy, Shandong Agricultural University, Tai'an 271018, P.R. ChinaState Key Laboratory of Crop Biology/Shandong Key Laboratory of Crop Biology/College of Agronomy, Shandong Agricultural University, Tai'an 271018, P.R. ChinaState Key Laboratory of Crop Biology/Shandong Key Laboratory of Crop Biology/College of Agronomy, Shandong Agricultural University, Tai'an 271018, P.R. ChinaState Key Laboratory of Crop Biology/Shandong Key Laboratory of Crop Biology/College of Agronomy, Shandong Agricultural University, Tai'an 271018, P.R. ChinaState Key Laboratory of Crop Biology/Shandong Key Laboratory of Crop Biology/College of Agronomy, Shandong Agricultural University, Tai'an 271018, P.R. ChinaState Key Laboratory of Crop Biology/Shandong Key Laboratory of Crop Biology/College of Agronomy, Shandong Agricultural University, Tai'an 271018, P.R. ChinaState Key Laboratory of Crop Biology/Shandong Key Laboratory of Crop Biology/College of Agronomy, Shandong Agricultural University, Tai'an 271018, P.R. China; Correspondence KONG Ling-rang, Tel: +86-538-8249278, Fax: +86-538-8242226The mutation of the gene encoding a stearoyl-acyl carrier protein fatty acid desaturase (ssi2) has been proved to enhance pathogen resistance in several plants, while it's potential to regulate biotic and abiotic stresses in wheat is still unclear. In this study, we cloned TaSSI2 gene in wheat and provided several evidences of its involvement in multiple biological functions. By using barley stripe mosaic virus (BSMV)-induced gene silencing (VIGS) in wheat, it was found that TaSSI2 negatively regulated both powdery mildew and Fusarium head blight (FHB) resistance, which was consistent with the phenotype observed in knock-out mutants of Kronos. The expression of TaSSI2 was down-regulated by in vitro treatments of methyl jasmonate (MeJA), but positively regulated by salicylic acid (SA) and abscisic acid (ABA), implying the cross-talk between different hormone signaling pathways involved in wheat to regulate biotic stresses is still to be elucidated. Furthermore, the up-regulated expression of PR4 and PR5 indicated that TaSSI2 probably regulated FHB resistance by depressing the SA signaling pathway in wheat. In addition, the over-expression of TaSSI2 increased the content of linolenic acid (18:3) and subsequently enhanced drought tolerance of transgenic Brachypodium. This phenomenon might be associated with its subcellular localization in the whole cytosol, partly overlapping with Golgi apparatus and the secreted vesicles. As a stearoyl-acyl carrier protein fatty acid desaturase, TaSSI2 was proposed to be involved in cell lipid metabolism and carried targets out of the cell from membrane or wax synthesis, resulting in enhanced drought tolerance in plant.http://www.sciencedirect.com/science/article/pii/S2095311917616800TaSSI2powdery mildewFusarium head blightdrought |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Li-qin HU Jing-jing MU Pei-sen SU Hong-yan WU Guang-hui YU Gui-ping WANG Liang WANG Xin MA An-fei LI Hong-wei WANG Lan-fei ZHAO Ling-rang KONG |
spellingShingle |
Li-qin HU Jing-jing MU Pei-sen SU Hong-yan WU Guang-hui YU Gui-ping WANG Liang WANG Xin MA An-fei LI Hong-wei WANG Lan-fei ZHAO Ling-rang KONG Multi-functional roles of TaSSI2 involved in Fusarium head blight and powdery mildew resistance and drought tolerance Journal of Integrative Agriculture TaSSI2 powdery mildew Fusarium head blight drought |
author_facet |
Li-qin HU Jing-jing MU Pei-sen SU Hong-yan WU Guang-hui YU Gui-ping WANG Liang WANG Xin MA An-fei LI Hong-wei WANG Lan-fei ZHAO Ling-rang KONG |
author_sort |
Li-qin HU |
title |
Multi-functional roles of TaSSI2 involved in Fusarium head blight and powdery mildew resistance and drought tolerance |
title_short |
Multi-functional roles of TaSSI2 involved in Fusarium head blight and powdery mildew resistance and drought tolerance |
title_full |
Multi-functional roles of TaSSI2 involved in Fusarium head blight and powdery mildew resistance and drought tolerance |
title_fullStr |
Multi-functional roles of TaSSI2 involved in Fusarium head blight and powdery mildew resistance and drought tolerance |
title_full_unstemmed |
Multi-functional roles of TaSSI2 involved in Fusarium head blight and powdery mildew resistance and drought tolerance |
title_sort |
multi-functional roles of tassi2 involved in fusarium head blight and powdery mildew resistance and drought tolerance |
publisher |
Elsevier |
series |
Journal of Integrative Agriculture |
issn |
2095-3119 |
publishDate |
2018-02-01 |
description |
The mutation of the gene encoding a stearoyl-acyl carrier protein fatty acid desaturase (ssi2) has been proved to enhance pathogen resistance in several plants, while it's potential to regulate biotic and abiotic stresses in wheat is still unclear. In this study, we cloned TaSSI2 gene in wheat and provided several evidences of its involvement in multiple biological functions. By using barley stripe mosaic virus (BSMV)-induced gene silencing (VIGS) in wheat, it was found that TaSSI2 negatively regulated both powdery mildew and Fusarium head blight (FHB) resistance, which was consistent with the phenotype observed in knock-out mutants of Kronos. The expression of TaSSI2 was down-regulated by in vitro treatments of methyl jasmonate (MeJA), but positively regulated by salicylic acid (SA) and abscisic acid (ABA), implying the cross-talk between different hormone signaling pathways involved in wheat to regulate biotic stresses is still to be elucidated. Furthermore, the up-regulated expression of PR4 and PR5 indicated that TaSSI2 probably regulated FHB resistance by depressing the SA signaling pathway in wheat. In addition, the over-expression of TaSSI2 increased the content of linolenic acid (18:3) and subsequently enhanced drought tolerance of transgenic Brachypodium. This phenomenon might be associated with its subcellular localization in the whole cytosol, partly overlapping with Golgi apparatus and the secreted vesicles. As a stearoyl-acyl carrier protein fatty acid desaturase, TaSSI2 was proposed to be involved in cell lipid metabolism and carried targets out of the cell from membrane or wax synthesis, resulting in enhanced drought tolerance in plant. |
topic |
TaSSI2 powdery mildew Fusarium head blight drought |
url |
http://www.sciencedirect.com/science/article/pii/S2095311917616800 |
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