Colorimetric Assay for 4-Phenylthiosemicarbazide Detection in Environmental Samples Based on Prussian Blue Nanoparticles Formation Ion
In this research, a fast colorimetric method for the detection of 4-phenylthiosemicarbazid (4-PTSC) in environmental samples, is reported for the first time. Semicarbazide compounds have been used as pesticides or fungicides in agriculture and for chemical synthesis in industrial processes. With thi...
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Iranian Institute of Research and Development in Chemical Industries (IRDCI)-ACECR
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doaj-88a66f75dd3f48c0aba025e0309b14132020-11-25T02:42:03ZengIranian Institute of Research and Development in Chemical Industries (IRDCI)-ACECRIranian Journal of Chemistry & Chemical Engineering 1021-99861021-99862017-03-0136112513325197Colorimetric Assay for 4-Phenylthiosemicarbazide Detection in Environmental Samples Based on Prussian Blue Nanoparticles Formation IonAmir Hatamie0Behrooz Zargar1Amir Jalali2Hesam Ameri3Department of Pharmacology and Toxicology, School of Pharmacy and Toxicology Research Center, Jundishapur University of Medical Sciences, Ahvaz, I.R. IRANDepartment of Chemistry, Faculty of Sciences, Shahid Chamran University, Ahvaz, I.R. IRANDepartment of Pharmacology and Toxicology, School of Pharmacy and Toxicology Research Center, Jundishapur University of Medical Sciences, Ahvaz, I.R. IRANDepartment of Pharmacology and Toxicology, School of Pharmacy and Toxicology Research Center, Jundishapur University of Medical Sciences, Ahvaz, I.R. IRANIn this research, a fast colorimetric method for the detection of 4-phenylthiosemicarbazid (4-PTSC) in environmental samples, is reported for the first time. Semicarbazide compounds have been used as pesticides or fungicides in agriculture and for chemical synthesis in industrial processes. With this method, one or the total amount of Semicarbazide in real samples can be detected within 6-7 min. For this purpose, 4-PTSC was determined and can be used as a representative of the Semicarbazide family. The assay principle is based on the oxidation of 4-phenylthiosemicarbazid (as reducing agent) with Ferric ions in the presence of ferricyanide ion and triton X-100 as a stabilizer, as well as the formation of Prussian Blue NanoParticles (PBNPs). These nanoparticles exhibit a strong UV-Vis extinction band at 700 nm with high absorption coefficient (3.0× 104 M-1 cm-1 at 700 nm). A UV-Vis spectrophotometer is used to monitor changes in the absorption intensity of PBNPs. A change in the color of the solution can be easily observed by naked eyes, in the presence of sub-ppm levels of 4-PTSC, which is directly related to the 4-PTSC concentration. TEM images showed an average diameter of 30 nm. The effect of several reaction variables on the formation rate of PBNPs was studied. Beer's law was obeyed in the concentration range of 0.20-3.20 µg/mL. The detection limit was found to be 0.10 µg/mL. This method was successfully applied for the determination of 4-PTSC in different environmental samples. Interfering species with reducing properties are not commonly present in environmental samples. Hence, this optical assay is simple and has acceptable selectivity.http://www.ijcce.ac.ir/article_25197_a58d849ba2dfb2c2d6880cb26a563ed5.pdfspectrophotometric probe. pesticideprussian blue nanoparticlesenvironmental samples |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Amir Hatamie Behrooz Zargar Amir Jalali Hesam Ameri |
spellingShingle |
Amir Hatamie Behrooz Zargar Amir Jalali Hesam Ameri Colorimetric Assay for 4-Phenylthiosemicarbazide Detection in Environmental Samples Based on Prussian Blue Nanoparticles Formation Ion Iranian Journal of Chemistry & Chemical Engineering spectrophotometric probe. pesticide prussian blue nanoparticles environmental samples |
author_facet |
Amir Hatamie Behrooz Zargar Amir Jalali Hesam Ameri |
author_sort |
Amir Hatamie |
title |
Colorimetric Assay for 4-Phenylthiosemicarbazide Detection in Environmental Samples Based on Prussian Blue Nanoparticles Formation Ion |
title_short |
Colorimetric Assay for 4-Phenylthiosemicarbazide Detection in Environmental Samples Based on Prussian Blue Nanoparticles Formation Ion |
title_full |
Colorimetric Assay for 4-Phenylthiosemicarbazide Detection in Environmental Samples Based on Prussian Blue Nanoparticles Formation Ion |
title_fullStr |
Colorimetric Assay for 4-Phenylthiosemicarbazide Detection in Environmental Samples Based on Prussian Blue Nanoparticles Formation Ion |
title_full_unstemmed |
Colorimetric Assay for 4-Phenylthiosemicarbazide Detection in Environmental Samples Based on Prussian Blue Nanoparticles Formation Ion |
title_sort |
colorimetric assay for 4-phenylthiosemicarbazide detection in environmental samples based on prussian blue nanoparticles formation ion |
publisher |
Iranian Institute of Research and Development in Chemical Industries (IRDCI)-ACECR |
series |
Iranian Journal of Chemistry & Chemical Engineering |
issn |
1021-9986 1021-9986 |
publishDate |
2017-03-01 |
description |
In this research, a fast colorimetric method for the detection of 4-phenylthiosemicarbazid (4-PTSC) in environmental samples, is reported for the first time. Semicarbazide compounds have been used as pesticides or fungicides in agriculture and for chemical synthesis in industrial processes. With this method, one or the total amount of Semicarbazide in real samples can be detected within 6-7 min. For this purpose, 4-PTSC was determined and can be used as a representative of the Semicarbazide family. The assay principle is based on the oxidation of 4-phenylthiosemicarbazid (as reducing agent) with Ferric ions in the presence of ferricyanide ion and triton X-100 as a stabilizer, as well as the formation of Prussian Blue NanoParticles (PBNPs). These nanoparticles exhibit a strong UV-Vis extinction band at 700 nm with high absorption coefficient (3.0× 104 M-1 cm-1 at 700 nm). A UV-Vis spectrophotometer is used to monitor changes in the absorption intensity of PBNPs. A change in the color of the solution can be easily observed by naked eyes, in the presence of sub-ppm levels of 4-PTSC, which is directly related to the 4-PTSC concentration. TEM images showed an average diameter of 30 nm. The effect of several reaction variables on the formation rate of PBNPs was studied. Beer's law was obeyed in the concentration range of 0.20-3.20 µg/mL. The detection limit was found to be 0.10 µg/mL. This method was successfully applied for the determination of 4-PTSC in different environmental samples. Interfering species with reducing properties are not commonly present in environmental samples. Hence, this optical assay is simple and has acceptable selectivity. |
topic |
spectrophotometric probe. pesticide prussian blue nanoparticles environmental samples |
url |
http://www.ijcce.ac.ir/article_25197_a58d849ba2dfb2c2d6880cb26a563ed5.pdf |
work_keys_str_mv |
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