Effect of CD14/TLR4 antagonist on GnRH/LH secretion in ewe during central inflammation induced by intracerebroventricular administration of LPS

Abstract Background Immune stress induced by lipopolysaccharide (LPS) influences the gonadotropin-releasing hormone (GnRH)/luteinizing hormone (LH) secretion. Presence of LPS interacting Toll-like receptor (TLR) 4 in the hypothalamus may enable the direct action of LPS on the GnRH/LH secretion. So,...

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Main Authors: Karolina Haziak, Andrzej Przemysław Herman, Karolina Wojtulewicz, Bartosz Pawlina, Kamila Paczesna, Joanna Bochenek, Dorota Tomaszewska-Zaremba
Format: Article
Language:English
Published: BMC 2018-07-01
Series:Journal of Animal Science and Biotechnology
Subjects:
Ewe
LH
LPS
Online Access:http://link.springer.com/article/10.1186/s40104-018-0267-8
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spelling doaj-88c574a0e0bc443e8727388b8fe310aa2020-11-25T01:05:48ZengBMCJournal of Animal Science and Biotechnology2049-18912018-07-019111010.1186/s40104-018-0267-8Effect of CD14/TLR4 antagonist on GnRH/LH secretion in ewe during central inflammation induced by intracerebroventricular administration of LPSKarolina Haziak0Andrzej Przemysław Herman1Karolina Wojtulewicz2Bartosz Pawlina3Kamila Paczesna4Joanna Bochenek5Dorota Tomaszewska-Zaremba6The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of SciencesThe Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of SciencesThe Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of SciencesThe Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of SciencesThe Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of SciencesThe Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of SciencesThe Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of SciencesAbstract Background Immune stress induced by lipopolysaccharide (LPS) influences the gonadotropin-releasing hormone (GnRH)/luteinizing hormone (LH) secretion. Presence of LPS interacting Toll-like receptor (TLR) 4 in the hypothalamus may enable the direct action of LPS on the GnRH/LH secretion. So, the aim of the study was to investigate the influence of intracerebroventricular (icv) injection of TLR4 antagonist on GnRH/LH secretion in anestrous ewes during LPS-induced central inflammation. Animals were divided into three groups icv-treated with: Ringer-Locke solution, LPS and TLR4 antagonist followed by LPS. Results It was demonstrated that TLR4 antagonist reduced LPS-dependent suppression of GnRH gene expression in the preoptic area and in the medial basal hypothalamus, and suppression of receptor for GnRH gene expression in the anterior pituitary gland. It was also shown that TLR4 antagonist reduced suppression of LH release caused by icv injection of LPS. Central administration of LPS stimulated TLR4 gene expression in the medial basal hypothalamus. Conclusions It was indicated that blockade of TLR4 prevents the inhibitory effect of centrally acting LPS on the GnRH/LH secretion. This suggests that some negative effects of bacterial infection on the hypothalamic-pituitary-gonadal axis activity at the hypothalamic level may be caused by central action of LPS acting through TLR4.http://link.springer.com/article/10.1186/s40104-018-0267-8EweGnRHLHLPSTLR4 antagonist
collection DOAJ
language English
format Article
sources DOAJ
author Karolina Haziak
Andrzej Przemysław Herman
Karolina Wojtulewicz
Bartosz Pawlina
Kamila Paczesna
Joanna Bochenek
Dorota Tomaszewska-Zaremba
spellingShingle Karolina Haziak
Andrzej Przemysław Herman
Karolina Wojtulewicz
Bartosz Pawlina
Kamila Paczesna
Joanna Bochenek
Dorota Tomaszewska-Zaremba
Effect of CD14/TLR4 antagonist on GnRH/LH secretion in ewe during central inflammation induced by intracerebroventricular administration of LPS
Journal of Animal Science and Biotechnology
Ewe
GnRH
LH
LPS
TLR4 antagonist
author_facet Karolina Haziak
Andrzej Przemysław Herman
Karolina Wojtulewicz
Bartosz Pawlina
Kamila Paczesna
Joanna Bochenek
Dorota Tomaszewska-Zaremba
author_sort Karolina Haziak
title Effect of CD14/TLR4 antagonist on GnRH/LH secretion in ewe during central inflammation induced by intracerebroventricular administration of LPS
title_short Effect of CD14/TLR4 antagonist on GnRH/LH secretion in ewe during central inflammation induced by intracerebroventricular administration of LPS
title_full Effect of CD14/TLR4 antagonist on GnRH/LH secretion in ewe during central inflammation induced by intracerebroventricular administration of LPS
title_fullStr Effect of CD14/TLR4 antagonist on GnRH/LH secretion in ewe during central inflammation induced by intracerebroventricular administration of LPS
title_full_unstemmed Effect of CD14/TLR4 antagonist on GnRH/LH secretion in ewe during central inflammation induced by intracerebroventricular administration of LPS
title_sort effect of cd14/tlr4 antagonist on gnrh/lh secretion in ewe during central inflammation induced by intracerebroventricular administration of lps
publisher BMC
series Journal of Animal Science and Biotechnology
issn 2049-1891
publishDate 2018-07-01
description Abstract Background Immune stress induced by lipopolysaccharide (LPS) influences the gonadotropin-releasing hormone (GnRH)/luteinizing hormone (LH) secretion. Presence of LPS interacting Toll-like receptor (TLR) 4 in the hypothalamus may enable the direct action of LPS on the GnRH/LH secretion. So, the aim of the study was to investigate the influence of intracerebroventricular (icv) injection of TLR4 antagonist on GnRH/LH secretion in anestrous ewes during LPS-induced central inflammation. Animals were divided into three groups icv-treated with: Ringer-Locke solution, LPS and TLR4 antagonist followed by LPS. Results It was demonstrated that TLR4 antagonist reduced LPS-dependent suppression of GnRH gene expression in the preoptic area and in the medial basal hypothalamus, and suppression of receptor for GnRH gene expression in the anterior pituitary gland. It was also shown that TLR4 antagonist reduced suppression of LH release caused by icv injection of LPS. Central administration of LPS stimulated TLR4 gene expression in the medial basal hypothalamus. Conclusions It was indicated that blockade of TLR4 prevents the inhibitory effect of centrally acting LPS on the GnRH/LH secretion. This suggests that some negative effects of bacterial infection on the hypothalamic-pituitary-gonadal axis activity at the hypothalamic level may be caused by central action of LPS acting through TLR4.
topic Ewe
GnRH
LH
LPS
TLR4 antagonist
url http://link.springer.com/article/10.1186/s40104-018-0267-8
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