Acute toxicity profiling of the ethyl acetate fraction of Swietenia macrophylla seeds and in-vitro neuroprotectio
Swietenia macrophylla (SM) is a medicinally important plant found in tropical and subtropical regions of the world. The ethyl acetate fraction of the seeds of S. macrophylla (SMEAF) is reported to exhibit potent anticancer, antitumor, anti-inflammatory and antifeedant activities. Till date, there ha...
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doaj-88dc17d0db83402ebaad2db6cf3106d22020-11-24T23:38:40ZengElsevierSaudi Pharmaceutical Journal1319-01642017-02-0125219620510.1016/j.jsps.2016.05.002Acute toxicity profiling of the ethyl acetate fraction of Swietenia macrophylla seeds and in-vitro neuroprotectioMustak Sayyad0Ning Tiang1Yatinesh Kumari2Bey Hing Goh3Yogini Jaiswal4Rozita Rosli5Leonard Williams6Mohd Farooq Shaikh7Department of Medical Genetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, MalaysiaJeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway 46150, Selangor Darul Ehsan, MalaysiaJeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway 46150, Selangor Darul Ehsan, MalaysiaJeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway 46150, Selangor Darul Ehsan, MalaysiaCenter for Excellence in Post Harvest Technologies, The North Carolina Research Campus, 500 Laureate Way, Kannapolis, NC 2808, USADepartment of Medical Genetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, MalaysiaCenter for Excellence in Post Harvest Technologies, The North Carolina Research Campus, 500 Laureate Way, Kannapolis, NC 2808, USAJeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway 46150, Selangor Darul Ehsan, MalaysiaSwietenia macrophylla (SM) is a medicinally important plant found in tropical and subtropical regions of the world. The ethyl acetate fraction of the seeds of S. macrophylla (SMEAF) is reported to exhibit potent anticancer, antitumor, anti-inflammatory and antifeedant activities. Till date, there have been no studies reported on the acute oral toxicity profile of the ethyl acetate fraction of the seeds of SM. The objective of the present study was to determine the acute toxicity of SMEAF and evaluate the in-vitro neuroprotective activity of SMEAF using primary neuronal cell cultures. In acute oral toxicity study, the SMEAF did not produce any lethal signs of morbidity and mortality. Histo-pathological findings, support the safety of SMEAF, as there were no significant changes observed in any of the parameters studied. Based on the results obtained in MTT assay, we infer that SMEAF has a significant neuroprotective effect, as it increased the cell viability and exhibited protection to the neuronal cells against TBHP induced oxidative stress. Thus, SMEAF can be suggested for use in the development of herbal drug formulations with neuroprotective potential.http://www.sciencedirect.com/science/article/pii/S1319016416300305Swietenia macrophyllaEthyl acetate extractNeuroprotectionOxidative stressAcute oral toxicity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mustak Sayyad Ning Tiang Yatinesh Kumari Bey Hing Goh Yogini Jaiswal Rozita Rosli Leonard Williams Mohd Farooq Shaikh |
spellingShingle |
Mustak Sayyad Ning Tiang Yatinesh Kumari Bey Hing Goh Yogini Jaiswal Rozita Rosli Leonard Williams Mohd Farooq Shaikh Acute toxicity profiling of the ethyl acetate fraction of Swietenia macrophylla seeds and in-vitro neuroprotectio Saudi Pharmaceutical Journal Swietenia macrophylla Ethyl acetate extract Neuroprotection Oxidative stress Acute oral toxicity |
author_facet |
Mustak Sayyad Ning Tiang Yatinesh Kumari Bey Hing Goh Yogini Jaiswal Rozita Rosli Leonard Williams Mohd Farooq Shaikh |
author_sort |
Mustak Sayyad |
title |
Acute toxicity profiling of the ethyl acetate fraction of Swietenia macrophylla seeds and in-vitro neuroprotectio |
title_short |
Acute toxicity profiling of the ethyl acetate fraction of Swietenia macrophylla seeds and in-vitro neuroprotectio |
title_full |
Acute toxicity profiling of the ethyl acetate fraction of Swietenia macrophylla seeds and in-vitro neuroprotectio |
title_fullStr |
Acute toxicity profiling of the ethyl acetate fraction of Swietenia macrophylla seeds and in-vitro neuroprotectio |
title_full_unstemmed |
Acute toxicity profiling of the ethyl acetate fraction of Swietenia macrophylla seeds and in-vitro neuroprotectio |
title_sort |
acute toxicity profiling of the ethyl acetate fraction of swietenia macrophylla seeds and in-vitro neuroprotectio |
publisher |
Elsevier |
series |
Saudi Pharmaceutical Journal |
issn |
1319-0164 |
publishDate |
2017-02-01 |
description |
Swietenia macrophylla (SM) is a medicinally important plant found in tropical and subtropical regions of the world. The ethyl acetate fraction of the seeds of S. macrophylla (SMEAF) is reported to exhibit potent anticancer, antitumor, anti-inflammatory and antifeedant activities. Till date, there have been no studies reported on the acute oral toxicity profile of the ethyl acetate fraction of the seeds of SM. The objective of the present study was to determine the acute toxicity of SMEAF and evaluate the in-vitro neuroprotective activity of SMEAF using primary neuronal cell cultures. In acute oral toxicity study, the SMEAF did not produce any lethal signs of morbidity and mortality. Histo-pathological findings, support the safety of SMEAF, as there were no significant changes observed in any of the parameters studied. Based on the results obtained in MTT assay, we infer that SMEAF has a significant neuroprotective effect, as it increased the cell viability and exhibited protection to the neuronal cells against TBHP induced oxidative stress. Thus, SMEAF can be suggested for use in the development of herbal drug formulations with neuroprotective potential. |
topic |
Swietenia macrophylla Ethyl acetate extract Neuroprotection Oxidative stress Acute oral toxicity |
url |
http://www.sciencedirect.com/science/article/pii/S1319016416300305 |
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