Inhibitory Effect of Indigo Naturalis on Tumor Necrosis Factor-α-Induced Vascular Cell Adhesion Molecule-1 Expression in Human Umbilical Vein Endothelial Cells
The use of indigo naturalis to treat psoriasis has proved effective in our previous clinical studies. The present study was designed to examine the anti-inflammatory effect of indigo naturalis in primary cultured human umbilical vein endothelial cells (HUVECs). Pretreatment of cells with indigo natu...
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2010-09-01
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Online Access: | http://www.mdpi.com/1420-3049/15/9/6423/ |
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doaj-88df0ad7290844a58fc372b5a994dba72020-11-24T23:34:53ZengMDPI AGMolecules1420-30492010-09-011596423643510.3390/molecules15096423Inhibitory Effect of Indigo Naturalis on Tumor Necrosis Factor-α-Induced Vascular Cell Adhesion Molecule-1 Expression in Human Umbilical Vein Endothelial CellsHsin-Ning ChangJong-Hwei Su PangSien-Hung YangChi-Feng HungChi-Hsin ChiangYin-Ku LinTung-Yi LinThe use of indigo naturalis to treat psoriasis has proved effective in our previous clinical studies. The present study was designed to examine the anti-inflammatory effect of indigo naturalis in primary cultured human umbilical vein endothelial cells (HUVECs). Pretreatment of cells with indigo naturalis extract attenuated TNF-α-induced increase in Jurkat T cell adhesion to HUVECs as well as decreased the protein and messenger (m)RNA expression levels of vascular cell adhesion molecule-1 (VCAM-1) on HUVECs. Indigo naturalis extract also inhibited the protein expression of activator protein-1 (AP-1)/c-Jun, a critical transcription factor for the activation of VCAM-1 gene expression. Since the reduction of lymphocyte adhesion to vascular cells by indigo naturalis extract could subsequently reduce the inflammatory reactions caused by lymphocyte infiltration in the epidermal layer and help to improve psoriasis, this study provides a potential mechanism for the anti-inflammatory therapeutic effect of indigo naturalis extract in psoriasis. http://www.mdpi.com/1420-3049/15/9/6423/psoriasisindigo naturalishuman umbilical vein endothelial cellvascular cell adhesion-1c-Jun |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hsin-Ning Chang Jong-Hwei Su Pang Sien-Hung Yang Chi-Feng Hung Chi-Hsin Chiang Yin-Ku Lin Tung-Yi Lin |
spellingShingle |
Hsin-Ning Chang Jong-Hwei Su Pang Sien-Hung Yang Chi-Feng Hung Chi-Hsin Chiang Yin-Ku Lin Tung-Yi Lin Inhibitory Effect of Indigo Naturalis on Tumor Necrosis Factor-α-Induced Vascular Cell Adhesion Molecule-1 Expression in Human Umbilical Vein Endothelial Cells Molecules psoriasis indigo naturalis human umbilical vein endothelial cell vascular cell adhesion-1 c-Jun |
author_facet |
Hsin-Ning Chang Jong-Hwei Su Pang Sien-Hung Yang Chi-Feng Hung Chi-Hsin Chiang Yin-Ku Lin Tung-Yi Lin |
author_sort |
Hsin-Ning Chang |
title |
Inhibitory Effect of Indigo Naturalis on Tumor Necrosis Factor-α-Induced Vascular Cell Adhesion Molecule-1 Expression in Human Umbilical Vein Endothelial Cells |
title_short |
Inhibitory Effect of Indigo Naturalis on Tumor Necrosis Factor-α-Induced Vascular Cell Adhesion Molecule-1 Expression in Human Umbilical Vein Endothelial Cells |
title_full |
Inhibitory Effect of Indigo Naturalis on Tumor Necrosis Factor-α-Induced Vascular Cell Adhesion Molecule-1 Expression in Human Umbilical Vein Endothelial Cells |
title_fullStr |
Inhibitory Effect of Indigo Naturalis on Tumor Necrosis Factor-α-Induced Vascular Cell Adhesion Molecule-1 Expression in Human Umbilical Vein Endothelial Cells |
title_full_unstemmed |
Inhibitory Effect of Indigo Naturalis on Tumor Necrosis Factor-α-Induced Vascular Cell Adhesion Molecule-1 Expression in Human Umbilical Vein Endothelial Cells |
title_sort |
inhibitory effect of indigo naturalis on tumor necrosis factor-α-induced vascular cell adhesion molecule-1 expression in human umbilical vein endothelial cells |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2010-09-01 |
description |
The use of indigo naturalis to treat psoriasis has proved effective in our previous clinical studies. The present study was designed to examine the anti-inflammatory effect of indigo naturalis in primary cultured human umbilical vein endothelial cells (HUVECs). Pretreatment of cells with indigo naturalis extract attenuated TNF-α-induced increase in Jurkat T cell adhesion to HUVECs as well as decreased the protein and messenger (m)RNA expression levels of vascular cell adhesion molecule-1 (VCAM-1) on HUVECs. Indigo naturalis extract also inhibited the protein expression of activator protein-1 (AP-1)/c-Jun, a critical transcription factor for the activation of VCAM-1 gene expression. Since the reduction of lymphocyte adhesion to vascular cells by indigo naturalis extract could subsequently reduce the inflammatory reactions caused by lymphocyte infiltration in the epidermal layer and help to improve psoriasis, this study provides a potential mechanism for the anti-inflammatory therapeutic effect of indigo naturalis extract in psoriasis. |
topic |
psoriasis indigo naturalis human umbilical vein endothelial cell vascular cell adhesion-1 c-Jun |
url |
http://www.mdpi.com/1420-3049/15/9/6423/ |
work_keys_str_mv |
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