Transferability of Microsatellite Markers Developed in <i>Oenothera</i> spp. to the Invasive Species <i>Oenothera</i> <i>drummondii</i> Hook. (Onagraceae)
<i>Oenothera drummondii</i> Hook. (Onagraceae) has life-history traits that make it an invasive species. Native populations are distributed along the coastal dunes from North Carolina in the United States to Tabasco in the Gulf of Mexico. It has been reported as an invasive species in Sp...
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doaj-88f63440447e4a8c8cc59c903a926e672020-11-25T03:55:09ZengMDPI AGDiversity1424-28182020-10-011238738710.3390/d12100387Transferability of Microsatellite Markers Developed in <i>Oenothera</i> spp. to the Invasive Species <i>Oenothera</i> <i>drummondii</i> Hook. (Onagraceae)Raquel Hernández-Espinosa0Jorge González-Astorga1Alejandro Espinosa de los Monteros2Dánae Cabrera-Toledo3Juan B. Gallego-Fernández4Red de Biología Evolutiva, Instituto de Ecología A. C. Carretera Antigua a Coatepec No. 351, El Haya, Xalapa 91073, Veracruz, MexicoRed de Biología Evolutiva, Instituto de Ecología A. C. Carretera Antigua a Coatepec No. 351, El Haya, Xalapa 91073, Veracruz, MexicoRed de Biología Evolutiva, Instituto de Ecología A. C. Carretera Antigua a Coatepec No. 351, El Haya, Xalapa 91073, Veracruz, MexicoInstituto de Botánica, Departamento de Botánica y Zoología, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara, Zapopan 44600, MexicoDepartamento de Biología Vegetal y Ecología, Universidad de Sevilla, Sevilla 41012, Spain<i>Oenothera drummondii</i> Hook. (Onagraceae) has life-history traits that make it an invasive species. Native populations are distributed along the coastal dunes from North Carolina in the United States to Tabasco in the Gulf of Mexico. It has been reported as an invasive species in Spain, Israel, and China, where this species can successfully colonize and dominate if the environmental conditions are appropriate. In South Africa, Australia, New Zealand, and France, it is reported to be naturalized. In this study, 28 microsatellite markers developed for other <i>Oenothera</i> species were evaluated for cross-amplification in <i>O. drummondii</i>. Nine primers showed consistent amplification and were polymorphic. Polymorphism was assessed in three populations from both native and invaded areas. Results indicated generalized low genetic variability. Three loci showed significant deviations from the Hardy Weinberg equilibrium, associated with null alleles' presence. The observed heterozygosity and inbreeding coefficient reflected a generalized excess of homozygotes, particularly in the invaded population “El Dique,” likely due to allele fixation. High genetic differentiation was found between the three populations. These results highlight the accuracy of these markers for future population genetic studies in <i>O. drummondii</i>.https://www.mdpi.com/1424-2818/12/10/387genetic diversitygenetic structureinvasive plantcross-amplificationcoastal dunesmicrosatellite loci |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Raquel Hernández-Espinosa Jorge González-Astorga Alejandro Espinosa de los Monteros Dánae Cabrera-Toledo Juan B. Gallego-Fernández |
spellingShingle |
Raquel Hernández-Espinosa Jorge González-Astorga Alejandro Espinosa de los Monteros Dánae Cabrera-Toledo Juan B. Gallego-Fernández Transferability of Microsatellite Markers Developed in <i>Oenothera</i> spp. to the Invasive Species <i>Oenothera</i> <i>drummondii</i> Hook. (Onagraceae) Diversity genetic diversity genetic structure invasive plant cross-amplification coastal dunes microsatellite loci |
author_facet |
Raquel Hernández-Espinosa Jorge González-Astorga Alejandro Espinosa de los Monteros Dánae Cabrera-Toledo Juan B. Gallego-Fernández |
author_sort |
Raquel Hernández-Espinosa |
title |
Transferability of Microsatellite Markers Developed in <i>Oenothera</i> spp. to the Invasive Species <i>Oenothera</i> <i>drummondii</i> Hook. (Onagraceae) |
title_short |
Transferability of Microsatellite Markers Developed in <i>Oenothera</i> spp. to the Invasive Species <i>Oenothera</i> <i>drummondii</i> Hook. (Onagraceae) |
title_full |
Transferability of Microsatellite Markers Developed in <i>Oenothera</i> spp. to the Invasive Species <i>Oenothera</i> <i>drummondii</i> Hook. (Onagraceae) |
title_fullStr |
Transferability of Microsatellite Markers Developed in <i>Oenothera</i> spp. to the Invasive Species <i>Oenothera</i> <i>drummondii</i> Hook. (Onagraceae) |
title_full_unstemmed |
Transferability of Microsatellite Markers Developed in <i>Oenothera</i> spp. to the Invasive Species <i>Oenothera</i> <i>drummondii</i> Hook. (Onagraceae) |
title_sort |
transferability of microsatellite markers developed in <i>oenothera</i> spp. to the invasive species <i>oenothera</i> <i>drummondii</i> hook. (onagraceae) |
publisher |
MDPI AG |
series |
Diversity |
issn |
1424-2818 |
publishDate |
2020-10-01 |
description |
<i>Oenothera drummondii</i> Hook. (Onagraceae) has life-history traits that make it an invasive species. Native populations are distributed along the coastal dunes from North Carolina in the United States to Tabasco in the Gulf of Mexico. It has been reported as an invasive species in Spain, Israel, and China, where this species can successfully colonize and dominate if the environmental conditions are appropriate. In South Africa, Australia, New Zealand, and France, it is reported to be naturalized. In this study, 28 microsatellite markers developed for other <i>Oenothera</i> species were evaluated for cross-amplification in <i>O. drummondii</i>. Nine primers showed consistent amplification and were polymorphic. Polymorphism was assessed in three populations from both native and invaded areas. Results indicated generalized low genetic variability. Three loci showed significant deviations from the Hardy Weinberg equilibrium, associated with null alleles' presence. The observed heterozygosity and inbreeding coefficient reflected a generalized excess of homozygotes, particularly in the invaded population “El Dique,” likely due to allele fixation. High genetic differentiation was found between the three populations. These results highlight the accuracy of these markers for future population genetic studies in <i>O. drummondii</i>. |
topic |
genetic diversity genetic structure invasive plant cross-amplification coastal dunes microsatellite loci |
url |
https://www.mdpi.com/1424-2818/12/10/387 |
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