The Antimicrobial Efficacy of <em>Elaeis guineensis</em>: Characterization, <em>in Vitro</em> and <em>in Vivo</em> Studies
The urgent need to treat multi-drug resistant pathogenic microorganisms in chronically infected patients has given rise to the development of new antimicrobials from natural resources. We have tested <em>Elaeis guineensis </em>Jacq<em> </em>(Ar...
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doaj-88f99eade4e54b5ca18bc9a7e2f70eb02020-11-24T22:44:29ZengMDPI AGMolecules1420-30492012-04-0117548604877The Antimicrobial Efficacy of <em>Elaeis guineensis</em>: Characterization, <em>in Vitro</em> and <em>in Vivo</em> StudiesSreenivasan SasidharanJagat R. KanwarLachimanan Yoga LathaZuraini ZakariaYeng ChenSoundararajan VijayarathnaThe urgent need to treat multi-drug resistant pathogenic microorganisms in chronically infected patients has given rise to the development of new antimicrobials from natural resources. We have tested <em>Elaeis guineensis </em>Jacq<em> </em>(Arecaceae) methanol extract against a variety of bacterial, fungal and yeast strains associated with infections. Our studies have demonstrated that <em>E. guineensis</em> exhibits excellent antimicrobial activity <em>in vitro</em> and <em>in vivo</em> against the bacterial and fungal strains tested. A marked inhibitory effect of the <em>E. guineensis</em> extracts was observed against <em>C. albicans</em> whereby <em>E. guineensis</em> extract at ½, 1, or 2 times the MIC significantly inhibited <em>C. albicans</em> growth with a noticeable drop in optical density (OD) of the bacterial culture. This finding confirmed the anticandidal activity of the extract on <em>C. albicans</em>. Imaging using scanning (SEM) and transmission (TEM) electron microscopy was done to determine the major alterations in the microstructure of the extract-treated <em>C. albicans</em>. The main abnormalities noted via SEM and TEM studies were the alteration in morphology of the yeast cells. <em>In vivo</em> antimicrobial activity was studies in mice that had been inoculated with <em>C. albicans</em> and exhibited good anticandidal activity. The authors conclude that the extract may be used as a candidate for the development of anticandidal agent.<em> </em>http://www.mdpi.com/1420-3049/17/5/4860antimicrobial activity<em>Candida albicans</em><em>Elaeis guineensis</em><em> </em>scanning electron microscopytransmission electron microscopy |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sreenivasan Sasidharan Jagat R. Kanwar Lachimanan Yoga Latha Zuraini Zakaria Yeng Chen Soundararajan Vijayarathna |
spellingShingle |
Sreenivasan Sasidharan Jagat R. Kanwar Lachimanan Yoga Latha Zuraini Zakaria Yeng Chen Soundararajan Vijayarathna The Antimicrobial Efficacy of <em>Elaeis guineensis</em>: Characterization, <em>in Vitro</em> and <em>in Vivo</em> Studies Molecules antimicrobial activity <em>Candida albicans</em> <em>Elaeis guineensis</em> <em> </em>scanning electron microscopy transmission electron microscopy |
author_facet |
Sreenivasan Sasidharan Jagat R. Kanwar Lachimanan Yoga Latha Zuraini Zakaria Yeng Chen Soundararajan Vijayarathna |
author_sort |
Sreenivasan Sasidharan |
title |
The Antimicrobial Efficacy of <em>Elaeis guineensis</em>: Characterization, <em>in Vitro</em> and <em>in Vivo</em> Studies |
title_short |
The Antimicrobial Efficacy of <em>Elaeis guineensis</em>: Characterization, <em>in Vitro</em> and <em>in Vivo</em> Studies |
title_full |
The Antimicrobial Efficacy of <em>Elaeis guineensis</em>: Characterization, <em>in Vitro</em> and <em>in Vivo</em> Studies |
title_fullStr |
The Antimicrobial Efficacy of <em>Elaeis guineensis</em>: Characterization, <em>in Vitro</em> and <em>in Vivo</em> Studies |
title_full_unstemmed |
The Antimicrobial Efficacy of <em>Elaeis guineensis</em>: Characterization, <em>in Vitro</em> and <em>in Vivo</em> Studies |
title_sort |
antimicrobial efficacy of <em>elaeis guineensis</em>: characterization, <em>in vitro</em> and <em>in vivo</em> studies |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2012-04-01 |
description |
The urgent need to treat multi-drug resistant pathogenic microorganisms in chronically infected patients has given rise to the development of new antimicrobials from natural resources. We have tested <em>Elaeis guineensis </em>Jacq<em> </em>(Arecaceae) methanol extract against a variety of bacterial, fungal and yeast strains associated with infections. Our studies have demonstrated that <em>E. guineensis</em> exhibits excellent antimicrobial activity <em>in vitro</em> and <em>in vivo</em> against the bacterial and fungal strains tested. A marked inhibitory effect of the <em>E. guineensis</em> extracts was observed against <em>C. albicans</em> whereby <em>E. guineensis</em> extract at ½, 1, or 2 times the MIC significantly inhibited <em>C. albicans</em> growth with a noticeable drop in optical density (OD) of the bacterial culture. This finding confirmed the anticandidal activity of the extract on <em>C. albicans</em>. Imaging using scanning (SEM) and transmission (TEM) electron microscopy was done to determine the major alterations in the microstructure of the extract-treated <em>C. albicans</em>. The main abnormalities noted via SEM and TEM studies were the alteration in morphology of the yeast cells. <em>In vivo</em> antimicrobial activity was studies in mice that had been inoculated with <em>C. albicans</em> and exhibited good anticandidal activity. The authors conclude that the extract may be used as a candidate for the development of anticandidal agent.<em> </em> |
topic |
antimicrobial activity <em>Candida albicans</em> <em>Elaeis guineensis</em> <em> </em>scanning electron microscopy transmission electron microscopy |
url |
http://www.mdpi.com/1420-3049/17/5/4860 |
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