Diffusion coefficients and dissociation constants of enhanced green fluorescent protein binding to free standing membranes

Recently, a new and versatile assay to determine the partitioning coefficient KP as a measure for the affinity of peripheral membrane proteins for lipid bilayers was presented in the research article entitled, “Introducing a fluorescence-based standard to quantify protein partitioning into membranes...

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Main Authors:	Franziska A. Thomas, Ilaria Visco, Zdeněk Petrášek, Fabian Heinemann, Petra Schwille
Format:	Article
Language:	English
Published:	Elsevier 2015-12-01
Series:	Data in Brief
Online Access:	http://www.sciencedirect.com/science/article/pii/S2352340915002504

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spelling	doaj-88fe3b4b949c41aeabada6fbbab908a42020-11-25T01:14:51ZengElsevierData in Brief2352-34092015-12-015C53754110.1016/j.dib.2015.10.002Diffusion coefficients and dissociation constants of enhanced green fluorescent protein binding to free standing membranesFranziska A. Thomas0Ilaria Visco1Zdeněk Petrášek2Fabian Heinemann3Petra Schwille4Max Planck Institute of Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, GermanyMax Planck Institute of Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, GermanyMax Planck Institute of Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, GermanyMax Planck Institute of Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, GermanyMax Planck Institute of Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, GermanyRecently, a new and versatile assay to determine the partitioning coefficient KP as a measure for the affinity of peripheral membrane proteins for lipid bilayers was presented in the research article entitled, “Introducing a fluorescence-based standard to quantify protein partitioning into membranes” [1]. Here, the well-characterized binding of hexahistidine-tag (His6) to NTA(Ni) was utilized. Complementarily, this data article reports the average diffusion coefficient D of His6-tagged enhanced green fluorescent protein (eGFP-His6) and the fluorescent lipid analog ATTO‐647N‐DOPE in giant unilamellar vesicles (GUVs) containing different amounts of NTA(Ni) lipids. In addition, dissociation constants Kd of the NTA(Ni)/eGFP-His6 system are reported. Further, a conversion between Kd and KP is provided.http://www.sciencedirect.com/science/article/pii/S2352340915002504
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Franziska A. Thomas Ilaria Visco Zdeněk Petrášek Fabian Heinemann Petra Schwille
spellingShingle	Franziska A. Thomas Ilaria Visco Zdeněk Petrášek Fabian Heinemann Petra Schwille Diffusion coefficients and dissociation constants of enhanced green fluorescent protein binding to free standing membranes Data in Brief
author_facet	Franziska A. Thomas Ilaria Visco Zdeněk Petrášek Fabian Heinemann Petra Schwille
author_sort	Franziska A. Thomas
title	Diffusion coefficients and dissociation constants of enhanced green fluorescent protein binding to free standing membranes
title_short	Diffusion coefficients and dissociation constants of enhanced green fluorescent protein binding to free standing membranes
title_full	Diffusion coefficients and dissociation constants of enhanced green fluorescent protein binding to free standing membranes
title_fullStr	Diffusion coefficients and dissociation constants of enhanced green fluorescent protein binding to free standing membranes
title_full_unstemmed	Diffusion coefficients and dissociation constants of enhanced green fluorescent protein binding to free standing membranes
title_sort	diffusion coefficients and dissociation constants of enhanced green fluorescent protein binding to free standing membranes
publisher	Elsevier
series	Data in Brief
issn	2352-3409
publishDate	2015-12-01
description	Recently, a new and versatile assay to determine the partitioning coefficient KP as a measure for the affinity of peripheral membrane proteins for lipid bilayers was presented in the research article entitled, “Introducing a fluorescence-based standard to quantify protein partitioning into membranes” [1]. Here, the well-characterized binding of hexahistidine-tag (His6) to NTA(Ni) was utilized. Complementarily, this data article reports the average diffusion coefficient D of His6-tagged enhanced green fluorescent protein (eGFP-His6) and the fluorescent lipid analog ATTO‐647N‐DOPE in giant unilamellar vesicles (GUVs) containing different amounts of NTA(Ni) lipids. In addition, dissociation constants Kd of the NTA(Ni)/eGFP-His6 system are reported. Further, a conversion between Kd and KP is provided.
url	http://www.sciencedirect.com/science/article/pii/S2352340915002504
work_keys_str_mv	AT franziskaathomas diffusioncoefficientsanddissociationconstantsofenhancedgreenfluorescentproteinbindingtofreestandingmembranes AT ilariavisco diffusioncoefficientsanddissociationconstantsofenhancedgreenfluorescentproteinbindingtofreestandingmembranes AT zdenekpetrasek diffusioncoefficientsanddissociationconstantsofenhancedgreenfluorescentproteinbindingtofreestandingmembranes AT fabianheinemann diffusioncoefficientsanddissociationconstantsofenhancedgreenfluorescentproteinbindingtofreestandingmembranes AT petraschwille diffusioncoefficientsanddissociationconstantsofenhancedgreenfluorescentproteinbindingtofreestandingmembranes
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Diffusion coefficients and dissociation constants of enhanced green fluorescent protein binding to free standing membranes

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