Heat shock protein 90 is downregulated in calcific aortic valve disease
Abstract Background Calcific aortic valve disease (CAVD) is an atheroinflammatory process; finally it leads to progressive calcification of the valve. There is no effective pharmacological treatment for CAVD and many of the underlying molecular mechanisms remain unknown. We conducted a proteomic stu...
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doaj-89288b4c82b24f9b91558eb2b2448f6f2020-12-20T12:18:17ZengBMCBMC Cardiovascular Disorders1471-22612019-12-0119111210.1186/s12872-019-01294-2Heat shock protein 90 is downregulated in calcific aortic valve diseaseJonna Weisell0Pauli Ohukainen1Juha Näpänkangas2Steffen Ohlmeier3Ulrich Bergmann4Tuomas Peltonen5Panu Taskinen6Heikki Ruskoaho7Jaana Rysä8School of Pharmacy, University of Eastern FinlandResearch Unit of Biomedicine, Computational Medicine, University of OuluDepartment of Pathology, Cancer Research and Translational Medicine Research Unit, University of Oulu and Oulu University HospitalProteomics and Mass Spectrometry Core Facilities, Biocenter Oulu, Faculty of Biochemistry and Molecular Medicine, University of OuluProteomics and Mass Spectrometry Core Facilities, Biocenter Oulu, Faculty of Biochemistry and Molecular Medicine, University of OuluDepartment of Pharmacology and Toxicology, University of OuluDepartment of Cardiovascular Surgery, Oulu University Hospital, University of OuluDepartment of Pharmacology and Toxicology, University of OuluSchool of Pharmacy, University of Eastern FinlandAbstract Background Calcific aortic valve disease (CAVD) is an atheroinflammatory process; finally it leads to progressive calcification of the valve. There is no effective pharmacological treatment for CAVD and many of the underlying molecular mechanisms remain unknown. We conducted a proteomic study to reveal novel factors associated with CAVD. Methods We compared aortic valves from patients undergoing valvular replacement surgery due to non-calcified aortic insufficiency (control group, n = 5) to a stenotic group (n = 7) using two-dimensional difference gel electrophoresis (2D-DIGE). Protein spots were identified with mass spectrometry. Western blot and immunohistochemistry were used to validate the results in a separate patient cohort and Ingenuity Pathway Analysis (IPA) was exploited to predict the regulatory network of CAVD. Results We detected an upregulation of complement 9 (C9), serum amyloid P-component (APCS) and transgelin as well as downregulation of heat shock protein (HSP90), protein disulfide isomerase A3 (PDIA3), annexin A2 (ANXA2) and galectin-1 in patients with aortic valve stenosis. The decreased protein expression of HSP90 was confirmed with Western blot. Conclusions We describe here a novel data set of proteomic changes associated with CAVD, including downregulation of the pro-inflammatory cytosolic protein, HSP90.https://doi.org/10.1186/s12872-019-01294-2Aortic valve stenosisCalcified aortic valve diseaseHeat-shock proteinProteomics |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jonna Weisell Pauli Ohukainen Juha Näpänkangas Steffen Ohlmeier Ulrich Bergmann Tuomas Peltonen Panu Taskinen Heikki Ruskoaho Jaana Rysä |
spellingShingle |
Jonna Weisell Pauli Ohukainen Juha Näpänkangas Steffen Ohlmeier Ulrich Bergmann Tuomas Peltonen Panu Taskinen Heikki Ruskoaho Jaana Rysä Heat shock protein 90 is downregulated in calcific aortic valve disease BMC Cardiovascular Disorders Aortic valve stenosis Calcified aortic valve disease Heat-shock protein Proteomics |
author_facet |
Jonna Weisell Pauli Ohukainen Juha Näpänkangas Steffen Ohlmeier Ulrich Bergmann Tuomas Peltonen Panu Taskinen Heikki Ruskoaho Jaana Rysä |
author_sort |
Jonna Weisell |
title |
Heat shock protein 90 is downregulated in calcific aortic valve disease |
title_short |
Heat shock protein 90 is downregulated in calcific aortic valve disease |
title_full |
Heat shock protein 90 is downregulated in calcific aortic valve disease |
title_fullStr |
Heat shock protein 90 is downregulated in calcific aortic valve disease |
title_full_unstemmed |
Heat shock protein 90 is downregulated in calcific aortic valve disease |
title_sort |
heat shock protein 90 is downregulated in calcific aortic valve disease |
publisher |
BMC |
series |
BMC Cardiovascular Disorders |
issn |
1471-2261 |
publishDate |
2019-12-01 |
description |
Abstract Background Calcific aortic valve disease (CAVD) is an atheroinflammatory process; finally it leads to progressive calcification of the valve. There is no effective pharmacological treatment for CAVD and many of the underlying molecular mechanisms remain unknown. We conducted a proteomic study to reveal novel factors associated with CAVD. Methods We compared aortic valves from patients undergoing valvular replacement surgery due to non-calcified aortic insufficiency (control group, n = 5) to a stenotic group (n = 7) using two-dimensional difference gel electrophoresis (2D-DIGE). Protein spots were identified with mass spectrometry. Western blot and immunohistochemistry were used to validate the results in a separate patient cohort and Ingenuity Pathway Analysis (IPA) was exploited to predict the regulatory network of CAVD. Results We detected an upregulation of complement 9 (C9), serum amyloid P-component (APCS) and transgelin as well as downregulation of heat shock protein (HSP90), protein disulfide isomerase A3 (PDIA3), annexin A2 (ANXA2) and galectin-1 in patients with aortic valve stenosis. The decreased protein expression of HSP90 was confirmed with Western blot. Conclusions We describe here a novel data set of proteomic changes associated with CAVD, including downregulation of the pro-inflammatory cytosolic protein, HSP90. |
topic |
Aortic valve stenosis Calcified aortic valve disease Heat-shock protein Proteomics |
url |
https://doi.org/10.1186/s12872-019-01294-2 |
work_keys_str_mv |
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