Disinfection effect of microwave radiation on Bacillus subtilis as indicator organism on contaminated dental stone casts under dry and wet conditions
Objective: The disinfection of dental stone casts using microwave radiation has been shown, but doubts remain regarding its efficacy under various conditions. The aim of the present study was to evaluate the efficacy of microwave disinfection on wet and dry dental stone casts contaminated by a resis...
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German Medical Science GMS Publishing House
2017-08-01
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doaj-894d6a648fbd469f8a57c23f65f64fe82020-11-25T03:27:14ZdeuGerman Medical Science GMS Publishing HouseGMS Hygiene and Infection Control2196-52262017-08-0112Doc0910.3205/dgkh000294Disinfection effect of microwave radiation on Bacillus subtilis as indicator organism on contaminated dental stone casts under dry and wet conditionsRobati Anaraki, Mahmood0Mahboubi, Shiva1Pirzadeh, Tahereh2Lotfipour, Farzaneh3Torkamanzad, Naimeh4Dental and Periodontal Research Center, Tabriz University of Medical Science, Tabriz, IranDepartment of Prosthetic Dentistry, Dental School, Kurdistan University of Medical Science, Sanandaj, IranDepartment of Microbiology, Medical School, Tabriz University of Medical Science, Tabriz, IranFaculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, IranDental School, Tabriz University of Medical Science, Tabriz, IranObjective: The disinfection of dental stone casts using microwave radiation has been shown, but doubts remain regarding its efficacy under various conditions. The aim of the present study was to evaluate the efficacy of microwave disinfection on wet and dry dental stone casts contaminated by a resistant microorganism. Material and methods: In this in vitro study, 34 stone half-casts were prepared, contaminated with and divided into two groups. After drying the specimens of one group for 15 minutes using 450 W microwave energy, all the wet and dry specimens were exposed to 900 W microwave energy for 5 minutes. Specimens were then individually transferred to nutrient broth culture medium and after , one milliliter from each tube was cultured in nutrient agar media for 24 hours, and the colonies were counted in CFU/mL. Data were analyzed using multifactorial ANOVA and Bonferroni tests.Results: Casts in both wet and dry groups were disinfected to a high level (6 log), with no statistically significant differences between them (P<0.05). Conclusion: According to the results, microwave irradiation can disinfect dental stone casts to a high degree, irrespective of moisture level. However, the result should be confirmed by exploring with other species of resistant microorganisms.http://www.egms.de/static/en/journals/dgkh/2017-12/dgkh000294.shtmlBacillus subtilisdisinfectionstone castmicrowavesmoist and dry conditions |
collection |
DOAJ |
language |
deu |
format |
Article |
sources |
DOAJ |
author |
Robati Anaraki, Mahmood Mahboubi, Shiva Pirzadeh, Tahereh Lotfipour, Farzaneh Torkamanzad, Naimeh |
spellingShingle |
Robati Anaraki, Mahmood Mahboubi, Shiva Pirzadeh, Tahereh Lotfipour, Farzaneh Torkamanzad, Naimeh Disinfection effect of microwave radiation on Bacillus subtilis as indicator organism on contaminated dental stone casts under dry and wet conditions GMS Hygiene and Infection Control Bacillus subtilis disinfection stone cast microwaves moist and dry conditions |
author_facet |
Robati Anaraki, Mahmood Mahboubi, Shiva Pirzadeh, Tahereh Lotfipour, Farzaneh Torkamanzad, Naimeh |
author_sort |
Robati Anaraki, Mahmood |
title |
Disinfection effect of microwave radiation on Bacillus subtilis as indicator organism on contaminated dental stone casts under dry and wet conditions |
title_short |
Disinfection effect of microwave radiation on Bacillus subtilis as indicator organism on contaminated dental stone casts under dry and wet conditions |
title_full |
Disinfection effect of microwave radiation on Bacillus subtilis as indicator organism on contaminated dental stone casts under dry and wet conditions |
title_fullStr |
Disinfection effect of microwave radiation on Bacillus subtilis as indicator organism on contaminated dental stone casts under dry and wet conditions |
title_full_unstemmed |
Disinfection effect of microwave radiation on Bacillus subtilis as indicator organism on contaminated dental stone casts under dry and wet conditions |
title_sort |
disinfection effect of microwave radiation on bacillus subtilis as indicator organism on contaminated dental stone casts under dry and wet conditions |
publisher |
German Medical Science GMS Publishing House |
series |
GMS Hygiene and Infection Control |
issn |
2196-5226 |
publishDate |
2017-08-01 |
description |
Objective: The disinfection of dental stone casts using microwave radiation has been shown, but doubts remain regarding its efficacy under various conditions. The aim of the present study was to evaluate the efficacy of microwave disinfection on wet and dry dental stone casts contaminated by a resistant microorganism. Material and methods: In this in vitro study, 34 stone half-casts were prepared, contaminated with and divided into two groups. After drying the specimens of one group for 15 minutes using 450 W microwave energy, all the wet and dry specimens were exposed to 900 W microwave energy for 5 minutes. Specimens were then individually transferred to nutrient broth culture medium and after , one milliliter from each tube was cultured in nutrient agar media for 24 hours, and the colonies were counted in CFU/mL. Data were analyzed using multifactorial ANOVA and Bonferroni tests.Results: Casts in both wet and dry groups were disinfected to a high level (6 log), with no statistically significant differences between them (P<0.05). Conclusion: According to the results, microwave irradiation can disinfect dental stone casts to a high degree, irrespective of moisture level. However, the result should be confirmed by exploring with other species of resistant microorganisms. |
topic |
Bacillus subtilis disinfection stone cast microwaves moist and dry conditions |
url |
http://www.egms.de/static/en/journals/dgkh/2017-12/dgkh000294.shtml |
work_keys_str_mv |
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