The relationship between IL-10 (-819 C/T) polymorphism and Hepatitis B infection
Background and Aim: Anti-inflammatory cytokine IL-10 is important and single nucleotide polymorphisms in the interleukin-10 gene promoter is associated with HBV infection. Especially single nucleotide polymorphisms in the promoter region of the gene IL10, at position -1082, -819, -592 is associated...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Farname
2016-08-01
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| Series: | Iranian Journal of Medical Microbiology |
| Subjects: | |
| Online Access: | http://ijmm.ir/article-1-472-en.html |
| Summary: | Background and Aim: Anti-inflammatory cytokine IL-10 is important and single nucleotide polymorphisms in the interleukin-10 gene promoter is associated with HBV infection. Especially single nucleotide polymorphisms in the promoter region of the gene IL10, at position -1082, -819, -592 is associated with production of IL10. Therefore, the present study was to determine the effect of IL-10 -819 polymorphism with chronic HBV infection in Iranian patients.
Materials and Methods: A total of 200 subjects (100 chronically infected hepatitis B patients and 100 blood donors) with an age range of 18-59 years were included in this study randomly.The study in 1392 on samples of patients referred to clinical laboratory blood transfusion was performed. Genomic DNA was extracted from Buffy coat layer with using of salting out procedure.Allele Specific Polymerase Chain Reaction Method was used for determining polymorphisms -819 (C/T) in the IL-10 gene promoter.Electrophoresis was performed on a arose gel 1.5 per cent for the detection of PCR products. Data were analyzed using Chi Square analysis
Results: The frequency of Homozygote genotype IL-10 -819 (TT, TC, CC) was in patients with HBV 84%,12% and 4% and 89%,10% and 1% in healthy individuals respectively and no significant difference was observed between the two groups in terms of genotype (P =0.8). Variant of C allele frequency was in patients with HBV 10% and 6% in healthy individuals. There was no statistically significant difference between the two groups (P=.0.6).
Conclusions: Our results showed that there was no correlation between polymorphism IL-10-819(T/C) in control group and chronic Hepatitis B infection. |
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| ISSN: | 1735-8612 2345-4342 |