Determination of the Thermodegradation of deoxyArbutin in Aqueous Solution by High Performance Liquid Chromatography
Tyrosinase is the key and rate-limiting enzyme responsible for the conversion of tyrosine into melanin. Competitive inhibition of tyrosinase enzymatic activity results in decreased or absent melanin synthesis by melanocytes in human skin. DeoxyArbutin (4-[(tetrahydro-2H-pyran-2-yl)oxy]phenol), a nov...
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doaj-898301adccc145a58cc7e229aa3da89a2020-11-24T21:50:59ZengMDPI AGInternational Journal of Molecular Sciences1422-00672010-10-0111103977398710.3390/ijms11103977Determination of the Thermodegradation of deoxyArbutin in Aqueous Solution by High Performance Liquid ChromatographyChih-Chien LinChao-Hsun YangYi-Shyan ChenWilly W. L. HongJeng-Shiow LaiTyrosinase is the key and rate-limiting enzyme responsible for the conversion of tyrosine into melanin. Competitive inhibition of tyrosinase enzymatic activity results in decreased or absent melanin synthesis by melanocytes in human skin. DeoxyArbutin (4-[(tetrahydro-2H-pyran-2-yl)oxy]phenol), a novel skin whitening agent, was synthesized through the removal of hydroxyl groups from the glucose side-chain of arbutin. DeoxyArbutin not only shows greater inhibition of tyrosinase activity but is also safer than hydroquinone and arbutin. Hence, deoxyArbutin is a potential skin whitening agent for cosmetics and depigmenting drugs; however, stability of this compound under some conditions remains a problem. The lack of stability poses developmental and practical difficulties for the use of deoxyArbutin in cosmetics and medicines. Improving the thermostability of deoxyArbutin is an important issue for its development. In this research, we established an analytical procedure to verify the amount of deoxyArbutin in solutions using a high performance liquid chromatographic (HPLC) method. The results indicate that this novel skin whitening agent is a thermolabile compound in aqueous solutions. Additionally, the rate constant for thermodegradation (k) and the half-life (t1/2) of deoxyArbutin were determined and can be used to understand the thermodegradation kinetics of deoxyArbutin. This information can aid in the application of deoxyArbutin for many future uses. http://www.mdpi.com/1422-0067/11/10/3977/deoxyArbutinhydroquinoneskin whiteningthermostabilitytyrosinase inhibitor |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chih-Chien Lin Chao-Hsun Yang Yi-Shyan Chen Willy W. L. Hong Jeng-Shiow Lai |
spellingShingle |
Chih-Chien Lin Chao-Hsun Yang Yi-Shyan Chen Willy W. L. Hong Jeng-Shiow Lai Determination of the Thermodegradation of deoxyArbutin in Aqueous Solution by High Performance Liquid Chromatography International Journal of Molecular Sciences deoxyArbutin hydroquinone skin whitening thermostability tyrosinase inhibitor |
author_facet |
Chih-Chien Lin Chao-Hsun Yang Yi-Shyan Chen Willy W. L. Hong Jeng-Shiow Lai |
author_sort |
Chih-Chien Lin |
title |
Determination of the Thermodegradation of deoxyArbutin in Aqueous Solution by High Performance Liquid Chromatography |
title_short |
Determination of the Thermodegradation of deoxyArbutin in Aqueous Solution by High Performance Liquid Chromatography |
title_full |
Determination of the Thermodegradation of deoxyArbutin in Aqueous Solution by High Performance Liquid Chromatography |
title_fullStr |
Determination of the Thermodegradation of deoxyArbutin in Aqueous Solution by High Performance Liquid Chromatography |
title_full_unstemmed |
Determination of the Thermodegradation of deoxyArbutin in Aqueous Solution by High Performance Liquid Chromatography |
title_sort |
determination of the thermodegradation of deoxyarbutin in aqueous solution by high performance liquid chromatography |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2010-10-01 |
description |
Tyrosinase is the key and rate-limiting enzyme responsible for the conversion of tyrosine into melanin. Competitive inhibition of tyrosinase enzymatic activity results in decreased or absent melanin synthesis by melanocytes in human skin. DeoxyArbutin (4-[(tetrahydro-2H-pyran-2-yl)oxy]phenol), a novel skin whitening agent, was synthesized through the removal of hydroxyl groups from the glucose side-chain of arbutin. DeoxyArbutin not only shows greater inhibition of tyrosinase activity but is also safer than hydroquinone and arbutin. Hence, deoxyArbutin is a potential skin whitening agent for cosmetics and depigmenting drugs; however, stability of this compound under some conditions remains a problem. The lack of stability poses developmental and practical difficulties for the use of deoxyArbutin in cosmetics and medicines. Improving the thermostability of deoxyArbutin is an important issue for its development. In this research, we established an analytical procedure to verify the amount of deoxyArbutin in solutions using a high performance liquid chromatographic (HPLC) method. The results indicate that this novel skin whitening agent is a thermolabile compound in aqueous solutions. Additionally, the rate constant for thermodegradation (k) and the half-life (t1/2) of deoxyArbutin were determined and can be used to understand the thermodegradation kinetics of deoxyArbutin. This information can aid in the application of deoxyArbutin for many future uses. |
topic |
deoxyArbutin hydroquinone skin whitening thermostability tyrosinase inhibitor |
url |
http://www.mdpi.com/1422-0067/11/10/3977/ |
work_keys_str_mv |
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