Diversity of low-molecular-weight-glutenin subunit genes associated with D-genome in Triticum aestivum, Aegilops crassa, A. cylindrica and A. tauschii
The 40% of endosperm protein of common wheat is composed of Low Molecular Weight (LMW) Glutenin Subunits. To examine variation in the D genome of wheat, 98 accessions from different areas of Iran were studied using the five Glu-D3-specific pair primers. The amplification percentages of all primer pa...
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doaj-89a4c2bf21ef4697a3121a811650d7482020-11-24T21:49:03ZengAzarian JournalsAzarian Journal of Agriculture2383-44202016-09-01312227Diversity of low-molecular-weight-glutenin subunit genes associated with D-genome in Triticum aestivum, Aegilops crassa, A. cylindrica and A. tauschii Fatemeh Vafadar Shamasbi0Mohamadreza Naghavi1Sayvan Ahmadi2Ali-Akbar Shahnejat-Bushehri3Agricultural Biotechnology Department, Islamic Azad University, Maragheh branch, Maragheh, IranAgronomy and Plant Breeding Department, Agricultural College, University of Tehran, Karaj, IranBreeding and Biotechnology Department, Faculty of Agriculture, University of Tabriz, Tabriz, IranAgronomy and Plant Breeding Department, Agricultural College, University of Tehran, Karaj, IranThe 40% of endosperm protein of common wheat is composed of Low Molecular Weight (LMW) Glutenin Subunits. To examine variation in the D genome of wheat, 98 accessions from different areas of Iran were studied using the five Glu-D3-specific pair primers. The amplification percentages of all primer pair sets were 80.61%, 92.86%, 79.59%, 90.82% and 67.35%, respectively. In comparison of the four species, the most observed bands of the first primer pair were found in Ae. tauschii samples. For the second primer pair, the most frequency of the amplified product was found in the T. aestivum samples. For the third primer pair, the Ae. cylindrical samples had the most amplified band. For the fourth primer pair, the most amplified band was found in the T. aestivum samples. The Ae. cylindrica samples had the most frequency band for the fifth primer pair. Based on dendrogram analysis, the accessions were divided in to 18 categories; and also 42 accessions had a bond for any PCR reaction. It is hoped that the result will be effective in molecular analysis and breeding of native landrace plants.http://azarianjournals.ir/wp-content/uploads/aja15122001.pdfD-genomeDiversityLMW Glutenin |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fatemeh Vafadar Shamasbi Mohamadreza Naghavi Sayvan Ahmadi Ali-Akbar Shahnejat-Bushehri |
spellingShingle |
Fatemeh Vafadar Shamasbi Mohamadreza Naghavi Sayvan Ahmadi Ali-Akbar Shahnejat-Bushehri Diversity of low-molecular-weight-glutenin subunit genes associated with D-genome in Triticum aestivum, Aegilops crassa, A. cylindrica and A. tauschii Azarian Journal of Agriculture D-genome Diversity LMW Glutenin |
author_facet |
Fatemeh Vafadar Shamasbi Mohamadreza Naghavi Sayvan Ahmadi Ali-Akbar Shahnejat-Bushehri |
author_sort |
Fatemeh Vafadar Shamasbi |
title |
Diversity of low-molecular-weight-glutenin subunit genes associated with D-genome in Triticum aestivum, Aegilops crassa, A. cylindrica and A. tauschii |
title_short |
Diversity of low-molecular-weight-glutenin subunit genes associated with D-genome in Triticum aestivum, Aegilops crassa, A. cylindrica and A. tauschii |
title_full |
Diversity of low-molecular-weight-glutenin subunit genes associated with D-genome in Triticum aestivum, Aegilops crassa, A. cylindrica and A. tauschii |
title_fullStr |
Diversity of low-molecular-weight-glutenin subunit genes associated with D-genome in Triticum aestivum, Aegilops crassa, A. cylindrica and A. tauschii |
title_full_unstemmed |
Diversity of low-molecular-weight-glutenin subunit genes associated with D-genome in Triticum aestivum, Aegilops crassa, A. cylindrica and A. tauschii |
title_sort |
diversity of low-molecular-weight-glutenin subunit genes associated with d-genome in triticum aestivum, aegilops crassa, a. cylindrica and a. tauschii |
publisher |
Azarian Journals |
series |
Azarian Journal of Agriculture |
issn |
2383-4420 |
publishDate |
2016-09-01 |
description |
The 40% of endosperm protein of common wheat is composed of Low Molecular Weight (LMW) Glutenin Subunits. To examine variation in the D genome of wheat, 98 accessions from different areas of Iran were studied using the five Glu-D3-specific pair primers. The amplification percentages of all primer pair sets were 80.61%, 92.86%, 79.59%, 90.82% and 67.35%, respectively. In comparison of the four species, the most observed bands of the first primer pair were found in Ae. tauschii samples. For the second primer pair, the most frequency of the amplified product was found in the T. aestivum samples. For the third primer pair, the Ae. cylindrical samples had the most amplified band. For the fourth primer pair, the most amplified band was found in the T. aestivum samples. The Ae. cylindrica samples had the most frequency band for the fifth primer pair. Based on dendrogram analysis, the accessions were divided in to 18 categories; and also 42 accessions had a bond for any PCR reaction. It is hoped that the result will be effective in molecular analysis and breeding of native landrace plants. |
topic |
D-genome Diversity LMW Glutenin |
url |
http://azarianjournals.ir/wp-content/uploads/aja15122001.pdf |
work_keys_str_mv |
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