Metabolic profile comparison between follicular fluid and serum in normal cows and those affected by ovarian cysts

The aim of this study was to carry out the metabolic profile comparison between follicular fluid and serum in normal cows and those affected by ovarian cysts (OC). After slaughtering, blood samples and follicular fluids from normal and cystic animals were collected and assayed using commercial kits...

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Main Authors: Nora Mimoune, Rachid Kaidi, Mohammed Hocine Benaissa, Mohamed Wail Bahouh, Ratiba Baazizi, Mohamed Yassine Azzouz
Format: Article
Language:English
Published: Ss.Cyril and Methodius University, Skopje 2019-10-01
Series:Macedonian Veterinary Review
Subjects:
Online Access:https://macvetrev.mk/LoadArticleContent?DOI=10.2478_macvetrev_2018_0030
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spelling doaj-89ab1650ebad4f59be0aeaafba0c4f002020-11-25T02:39:52ZengSs.Cyril and Methodius University, SkopjeMacedonian Veterinary Review 1409-76211857-74152019-10-01421515910.2478/macvetrev-2018-0030Metabolic profile comparison between follicular fluid and serum in normal cows and those affected by ovarian cystsNora Mimoune0Rachid Kaidi1Mohammed Hocine Benaissa2Mohamed Wail Bahouh3Ratiba Baazizi4Mohamed Yassine Azzouz5National High School of Veterinary Medicine, Bab-Ezzouar, Algiers, Algeria, Institute of Veterinary Sciences, LBRA, University of Blida 1, PB 270, Soumaa, Blida, AlgeriaInstitute of Veterinary Sciences, LBRA, University of Blida 1, PB 270, Soumaa, Blida, Algeria, School of Veterinary Medicine and Science, University of Nottingham, Nottingham, Leicestershire, United KingdomScientific and Technical Research Centre for Arid Areas (CRSTRA), Biophysical Station, Touggourt, AlgeriaNational High School of Veterinary Medicine, Bab-Ezzouar, Algiers, AlgeriaNational High School of Veterinary Medicine, Bab-Ezzouar, Algiers, AlgeriaInstitute of Veterinary Sciences, LBRA, University of Blida 1, PB 270, Soumaa, Blida, AlgeriaThe aim of this study was to carry out the metabolic profile comparison between follicular fluid and serum in normal cows and those affected by ovarian cysts (OC). After slaughtering, blood samples and follicular fluids from normal and cystic animals were collected and assayed using commercial kits to determine the concentrations of metabolites (glucose, total protein, total cholesterol, cortisol, triglycerides, urea, creatinine and insulin) and the liver enzymes activity. Data showed that OC were characterized by low levels of glucose, total protein, cholesterol and cortisol in cystic fluid, while urea concentrations were high compared to normal follicular fluid (P<0.001). On the other hand, serum assays of cystic animals revealed very low values of insulin and urea, whereas cortisol levels were relatively high in comparison with the serum of normal cows (P<0.001). Significant correlations between the serum and follicular fluid concentrations of normal cows were found for glucose (r=0.49), total cholesterol (r=0.31), cortisol (r=0.38) and total protein (r=0.63). The highest correlation was found for urea (r=0.86). On contrary, weak correlations were observed between metabolites concentrations in cystic fluid and in serum for normal and cystic cows. In conclusion, OC grow and persist in a metabolic environment, which differs from follicular fluid to blood. These changes may act together and/or separately to ensure the continuous development of OC. To understand a part of the mechanism, the authors propose a deep study about blood-follicle-barrier.https://macvetrev.mk/LoadArticleContent?DOI=10.2478_macvetrev_2018_0030ovarian cystfollicular fluidserummetabolic profile
collection DOAJ
language English
format Article
sources DOAJ
author Nora Mimoune
Rachid Kaidi
Mohammed Hocine Benaissa
Mohamed Wail Bahouh
Ratiba Baazizi
Mohamed Yassine Azzouz
spellingShingle Nora Mimoune
Rachid Kaidi
Mohammed Hocine Benaissa
Mohamed Wail Bahouh
Ratiba Baazizi
Mohamed Yassine Azzouz
Metabolic profile comparison between follicular fluid and serum in normal cows and those affected by ovarian cysts
Macedonian Veterinary Review
ovarian cyst
follicular fluid
serum
metabolic profile
author_facet Nora Mimoune
Rachid Kaidi
Mohammed Hocine Benaissa
Mohamed Wail Bahouh
Ratiba Baazizi
Mohamed Yassine Azzouz
author_sort Nora Mimoune
title Metabolic profile comparison between follicular fluid and serum in normal cows and those affected by ovarian cysts
title_short Metabolic profile comparison between follicular fluid and serum in normal cows and those affected by ovarian cysts
title_full Metabolic profile comparison between follicular fluid and serum in normal cows and those affected by ovarian cysts
title_fullStr Metabolic profile comparison between follicular fluid and serum in normal cows and those affected by ovarian cysts
title_full_unstemmed Metabolic profile comparison between follicular fluid and serum in normal cows and those affected by ovarian cysts
title_sort metabolic profile comparison between follicular fluid and serum in normal cows and those affected by ovarian cysts
publisher Ss.Cyril and Methodius University, Skopje
series Macedonian Veterinary Review
issn 1409-7621
1857-7415
publishDate 2019-10-01
description The aim of this study was to carry out the metabolic profile comparison between follicular fluid and serum in normal cows and those affected by ovarian cysts (OC). After slaughtering, blood samples and follicular fluids from normal and cystic animals were collected and assayed using commercial kits to determine the concentrations of metabolites (glucose, total protein, total cholesterol, cortisol, triglycerides, urea, creatinine and insulin) and the liver enzymes activity. Data showed that OC were characterized by low levels of glucose, total protein, cholesterol and cortisol in cystic fluid, while urea concentrations were high compared to normal follicular fluid (P<0.001). On the other hand, serum assays of cystic animals revealed very low values of insulin and urea, whereas cortisol levels were relatively high in comparison with the serum of normal cows (P<0.001). Significant correlations between the serum and follicular fluid concentrations of normal cows were found for glucose (r=0.49), total cholesterol (r=0.31), cortisol (r=0.38) and total protein (r=0.63). The highest correlation was found for urea (r=0.86). On contrary, weak correlations were observed between metabolites concentrations in cystic fluid and in serum for normal and cystic cows. In conclusion, OC grow and persist in a metabolic environment, which differs from follicular fluid to blood. These changes may act together and/or separately to ensure the continuous development of OC. To understand a part of the mechanism, the authors propose a deep study about blood-follicle-barrier.
topic ovarian cyst
follicular fluid
serum
metabolic profile
url https://macvetrev.mk/LoadArticleContent?DOI=10.2478_macvetrev_2018_0030
work_keys_str_mv AT noramimoune metabolicprofilecomparisonbetweenfollicularfluidandseruminnormalcowsandthoseaffectedbyovariancysts
AT rachidkaidi metabolicprofilecomparisonbetweenfollicularfluidandseruminnormalcowsandthoseaffectedbyovariancysts
AT mohammedhocinebenaissa metabolicprofilecomparisonbetweenfollicularfluidandseruminnormalcowsandthoseaffectedbyovariancysts
AT mohamedwailbahouh metabolicprofilecomparisonbetweenfollicularfluidandseruminnormalcowsandthoseaffectedbyovariancysts
AT ratibabaazizi metabolicprofilecomparisonbetweenfollicularfluidandseruminnormalcowsandthoseaffectedbyovariancysts
AT mohamedyassineazzouz metabolicprofilecomparisonbetweenfollicularfluidandseruminnormalcowsandthoseaffectedbyovariancysts
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