Metabolic profile comparison between follicular fluid and serum in normal cows and those affected by ovarian cysts
The aim of this study was to carry out the metabolic profile comparison between follicular fluid and serum in normal cows and those affected by ovarian cysts (OC). After slaughtering, blood samples and follicular fluids from normal and cystic animals were collected and assayed using commercial kits...
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Ss.Cyril and Methodius University, Skopje
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doaj-89ab1650ebad4f59be0aeaafba0c4f002020-11-25T02:39:52ZengSs.Cyril and Methodius University, SkopjeMacedonian Veterinary Review 1409-76211857-74152019-10-01421515910.2478/macvetrev-2018-0030Metabolic profile comparison between follicular fluid and serum in normal cows and those affected by ovarian cystsNora Mimoune0Rachid Kaidi1Mohammed Hocine Benaissa2Mohamed Wail Bahouh3Ratiba Baazizi4Mohamed Yassine Azzouz5National High School of Veterinary Medicine, Bab-Ezzouar, Algiers, Algeria, Institute of Veterinary Sciences, LBRA, University of Blida 1, PB 270, Soumaa, Blida, AlgeriaInstitute of Veterinary Sciences, LBRA, University of Blida 1, PB 270, Soumaa, Blida, Algeria, School of Veterinary Medicine and Science, University of Nottingham, Nottingham, Leicestershire, United KingdomScientific and Technical Research Centre for Arid Areas (CRSTRA), Biophysical Station, Touggourt, AlgeriaNational High School of Veterinary Medicine, Bab-Ezzouar, Algiers, AlgeriaNational High School of Veterinary Medicine, Bab-Ezzouar, Algiers, AlgeriaInstitute of Veterinary Sciences, LBRA, University of Blida 1, PB 270, Soumaa, Blida, AlgeriaThe aim of this study was to carry out the metabolic profile comparison between follicular fluid and serum in normal cows and those affected by ovarian cysts (OC). After slaughtering, blood samples and follicular fluids from normal and cystic animals were collected and assayed using commercial kits to determine the concentrations of metabolites (glucose, total protein, total cholesterol, cortisol, triglycerides, urea, creatinine and insulin) and the liver enzymes activity. Data showed that OC were characterized by low levels of glucose, total protein, cholesterol and cortisol in cystic fluid, while urea concentrations were high compared to normal follicular fluid (P<0.001). On the other hand, serum assays of cystic animals revealed very low values of insulin and urea, whereas cortisol levels were relatively high in comparison with the serum of normal cows (P<0.001). Significant correlations between the serum and follicular fluid concentrations of normal cows were found for glucose (r=0.49), total cholesterol (r=0.31), cortisol (r=0.38) and total protein (r=0.63). The highest correlation was found for urea (r=0.86). On contrary, weak correlations were observed between metabolites concentrations in cystic fluid and in serum for normal and cystic cows. In conclusion, OC grow and persist in a metabolic environment, which differs from follicular fluid to blood. These changes may act together and/or separately to ensure the continuous development of OC. To understand a part of the mechanism, the authors propose a deep study about blood-follicle-barrier.https://macvetrev.mk/LoadArticleContent?DOI=10.2478_macvetrev_2018_0030ovarian cystfollicular fluidserummetabolic profile |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nora Mimoune Rachid Kaidi Mohammed Hocine Benaissa Mohamed Wail Bahouh Ratiba Baazizi Mohamed Yassine Azzouz |
spellingShingle |
Nora Mimoune Rachid Kaidi Mohammed Hocine Benaissa Mohamed Wail Bahouh Ratiba Baazizi Mohamed Yassine Azzouz Metabolic profile comparison between follicular fluid and serum in normal cows and those affected by ovarian cysts Macedonian Veterinary Review ovarian cyst follicular fluid serum metabolic profile |
author_facet |
Nora Mimoune Rachid Kaidi Mohammed Hocine Benaissa Mohamed Wail Bahouh Ratiba Baazizi Mohamed Yassine Azzouz |
author_sort |
Nora Mimoune |
title |
Metabolic profile comparison between follicular fluid and serum in normal cows and those affected by ovarian cysts |
title_short |
Metabolic profile comparison between follicular fluid and serum in normal cows and those affected by ovarian cysts |
title_full |
Metabolic profile comparison between follicular fluid and serum in normal cows and those affected by ovarian cysts |
title_fullStr |
Metabolic profile comparison between follicular fluid and serum in normal cows and those affected by ovarian cysts |
title_full_unstemmed |
Metabolic profile comparison between follicular fluid and serum in normal cows and those affected by ovarian cysts |
title_sort |
metabolic profile comparison between follicular fluid and serum in normal cows and those affected by ovarian cysts |
publisher |
Ss.Cyril and Methodius University, Skopje |
series |
Macedonian Veterinary Review |
issn |
1409-7621 1857-7415 |
publishDate |
2019-10-01 |
description |
The aim of this study was to carry out the metabolic profile comparison between follicular fluid and serum in normal cows and those affected by ovarian cysts (OC). After slaughtering, blood samples and follicular fluids from normal and cystic animals were collected and assayed using commercial kits to determine the concentrations of metabolites (glucose, total protein, total cholesterol, cortisol, triglycerides, urea, creatinine and insulin) and the liver enzymes activity. Data showed that OC were characterized by low levels of glucose, total protein, cholesterol and cortisol in cystic fluid, while urea concentrations were high compared to normal follicular fluid (P<0.001). On the other hand, serum assays of cystic animals revealed very low values of insulin and urea, whereas cortisol levels were relatively high in comparison with the serum of normal cows (P<0.001). Significant correlations between the serum and follicular fluid concentrations of normal cows were found for glucose (r=0.49), total cholesterol (r=0.31), cortisol (r=0.38) and total protein (r=0.63). The highest correlation was found for urea (r=0.86). On contrary, weak correlations were observed between metabolites concentrations in cystic fluid and in serum for normal and cystic cows. In conclusion, OC grow and persist in a metabolic environment, which differs from follicular fluid to blood. These changes may act together and/or separately to ensure the continuous development of OC. To understand a part of the mechanism, the authors propose a deep study about blood-follicle-barrier. |
topic |
ovarian cyst follicular fluid serum metabolic profile |
url |
https://macvetrev.mk/LoadArticleContent?DOI=10.2478_macvetrev_2018_0030 |
work_keys_str_mv |
AT noramimoune metabolicprofilecomparisonbetweenfollicularfluidandseruminnormalcowsandthoseaffectedbyovariancysts AT rachidkaidi metabolicprofilecomparisonbetweenfollicularfluidandseruminnormalcowsandthoseaffectedbyovariancysts AT mohammedhocinebenaissa metabolicprofilecomparisonbetweenfollicularfluidandseruminnormalcowsandthoseaffectedbyovariancysts AT mohamedwailbahouh metabolicprofilecomparisonbetweenfollicularfluidandseruminnormalcowsandthoseaffectedbyovariancysts AT ratibabaazizi metabolicprofilecomparisonbetweenfollicularfluidandseruminnormalcowsandthoseaffectedbyovariancysts AT mohamedyassineazzouz metabolicprofilecomparisonbetweenfollicularfluidandseruminnormalcowsandthoseaffectedbyovariancysts |
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