Longitudinal Microbiome Analysis in a Dextran Sulfate Sodium-Induced Colitis Mouse Model
The role of the gut microbiota in the pathogenesis of inflammatory bowel disease (IBD) has been in focus for decades. Although metagenomic observations in patients/animal colitis models have been attempted, the microbiome results were still indefinite and broad taxonomic presumptions were made due t...
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doaj-89af706d0b7649659497676ca3cfc56b2021-02-13T00:04:59ZengMDPI AGMicroorganisms2076-26072021-02-01937037010.3390/microorganisms9020370Longitudinal Microbiome Analysis in a Dextran Sulfate Sodium-Induced Colitis Mouse ModelHyunjoon Park0Soyoung Yeo1Seokwon Kang2Chul Sung Huh3Research Institute of Eco-Friendly Livestock Science, Institute of Green-Bio Science and Technology, Seoul National University, Pyeongchang, Gangwon 25354, KoreaDepartment of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, KoreaDepartment of Life Sciences, Handong Global University, Pohang 37554, KoreaResearch Institute of Eco-Friendly Livestock Science, Institute of Green-Bio Science and Technology, Seoul National University, Pyeongchang, Gangwon 25354, KoreaThe role of the gut microbiota in the pathogenesis of inflammatory bowel disease (IBD) has been in focus for decades. Although metagenomic observations in patients/animal colitis models have been attempted, the microbiome results were still indefinite and broad taxonomic presumptions were made due to the cross-sectional studies. Herein, we conducted a longitudinal microbiome analysis in a dextran sulfate sodium (DSS)-induced colitis mouse model with a two-factor design based on serial DSS dose (0, 1, 2, and 3%) and duration for 12 days, and four mice from each group were sacrificed at two-day intervals. During the colitis development, a transition of the cecal microbial diversity from the normal state to dysbiosis and dynamic changes of the populations were observed. We identified genera that significantly induced or depleted depending on DSS exposure, and confirmed the correlations of the individual taxa to the colitis severity indicated by inflammatory biomarkers (intestinal bleeding and neutrophil-derived indicators). Of note, each taxonomic population showed its own susceptibility to the changing colitis status. Our findings suggest that an understanding of the individual susceptibility to colitis conditions may contribute to identifying the role of the gut microbes in the pathogenesis of IBD.https://www.mdpi.com/2076-2607/9/2/370intestinal microbiotaDSS-induced colitis developmentlongitudinal studiesdynamicssusceptibilitygut microbes |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hyunjoon Park Soyoung Yeo Seokwon Kang Chul Sung Huh |
spellingShingle |
Hyunjoon Park Soyoung Yeo Seokwon Kang Chul Sung Huh Longitudinal Microbiome Analysis in a Dextran Sulfate Sodium-Induced Colitis Mouse Model Microorganisms intestinal microbiota DSS-induced colitis development longitudinal studies dynamics susceptibility gut microbes |
author_facet |
Hyunjoon Park Soyoung Yeo Seokwon Kang Chul Sung Huh |
author_sort |
Hyunjoon Park |
title |
Longitudinal Microbiome Analysis in a Dextran Sulfate Sodium-Induced Colitis Mouse Model |
title_short |
Longitudinal Microbiome Analysis in a Dextran Sulfate Sodium-Induced Colitis Mouse Model |
title_full |
Longitudinal Microbiome Analysis in a Dextran Sulfate Sodium-Induced Colitis Mouse Model |
title_fullStr |
Longitudinal Microbiome Analysis in a Dextran Sulfate Sodium-Induced Colitis Mouse Model |
title_full_unstemmed |
Longitudinal Microbiome Analysis in a Dextran Sulfate Sodium-Induced Colitis Mouse Model |
title_sort |
longitudinal microbiome analysis in a dextran sulfate sodium-induced colitis mouse model |
publisher |
MDPI AG |
series |
Microorganisms |
issn |
2076-2607 |
publishDate |
2021-02-01 |
description |
The role of the gut microbiota in the pathogenesis of inflammatory bowel disease (IBD) has been in focus for decades. Although metagenomic observations in patients/animal colitis models have been attempted, the microbiome results were still indefinite and broad taxonomic presumptions were made due to the cross-sectional studies. Herein, we conducted a longitudinal microbiome analysis in a dextran sulfate sodium (DSS)-induced colitis mouse model with a two-factor design based on serial DSS dose (0, 1, 2, and 3%) and duration for 12 days, and four mice from each group were sacrificed at two-day intervals. During the colitis development, a transition of the cecal microbial diversity from the normal state to dysbiosis and dynamic changes of the populations were observed. We identified genera that significantly induced or depleted depending on DSS exposure, and confirmed the correlations of the individual taxa to the colitis severity indicated by inflammatory biomarkers (intestinal bleeding and neutrophil-derived indicators). Of note, each taxonomic population showed its own susceptibility to the changing colitis status. Our findings suggest that an understanding of the individual susceptibility to colitis conditions may contribute to identifying the role of the gut microbes in the pathogenesis of IBD. |
topic |
intestinal microbiota DSS-induced colitis development longitudinal studies dynamics susceptibility gut microbes |
url |
https://www.mdpi.com/2076-2607/9/2/370 |
work_keys_str_mv |
AT hyunjoonpark longitudinalmicrobiomeanalysisinadextransulfatesodiuminducedcolitismousemodel AT soyoungyeo longitudinalmicrobiomeanalysisinadextransulfatesodiuminducedcolitismousemodel AT seokwonkang longitudinalmicrobiomeanalysisinadextransulfatesodiuminducedcolitismousemodel AT chulsunghuh longitudinalmicrobiomeanalysisinadextransulfatesodiuminducedcolitismousemodel |
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