A Novel Type of Neuron Within the Dorsal Striatum
The striatum is predominantly composed of medium spiny projection neurons, with the remaining neurons consisting of several types of interneurons. Among the interneurons are a group of cells that express tyrosine hydroxylase (TH). Although the intrinsic electrical properties of these TH-expressing i...
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doaj-89d0a989084447f4af869728de8d70a82020-11-25T02:27:42ZengFrontiers Media S.A.Frontiers in Neural Circuits1662-51102019-05-011310.3389/fncir.2019.00032430182A Novel Type of Neuron Within the Dorsal StriatumMiaomiao Mao0Miaomiao Mao1Aditya Nair2Aditya Nair3Aditya Nair4Aditya Nair5George J. Augustine6George J. Augustine7Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore, SingaporeInstitute of Molecular and Cell Biology, Singapore, SingaporeLee Kong Chian School of Medicine, Nanyang Technological University, Singapore, SingaporeInstitute of Molecular and Cell Biology, Singapore, SingaporeLife Sciences Programme, Faculty of Science, National University of Singapore, Singapore, SingaporeSingapore Bioimaging Consortium, Agency of Science, Technology and Research, Singapore, SingaporeLee Kong Chian School of Medicine, Nanyang Technological University, Singapore, SingaporeInstitute of Molecular and Cell Biology, Singapore, SingaporeThe striatum is predominantly composed of medium spiny projection neurons, with the remaining neurons consisting of several types of interneurons. Among the interneurons are a group of cells that express tyrosine hydroxylase (TH). Although the intrinsic electrical properties of these TH-expressing interneurons have been characterized, there is no agreement on the number of TH-expressing cell types and their electrical properties. Here, we have used transgenic mice in which YFP-tagged channelrhodopsin-2 (ChR2) was expressed in potential TH-expressing cells in a Cre-dependent manner. We found that the YFP+ neurons in the striatum were heterogeneous in their intrinsic electrical properties; unbiased clustering indicated that there are three main neuronal subtypes. One population of neurons had aspiny dendrites with high-frequency action potential (AP) firing and plateau potentials, resembling the TH interneurons (THIN) described previously. A second, very small population of labeled neurons resembled medium-sized spiny neurons (MSN). The third population of neurons had dendrites with an intermediate density of spines, showed substantial AP adaptation and generated prolonged spikes. This type of striatal neuron has not been previously identified in the adult mouse and we have named it the Frequency-Adapting Neuron with Spines (FANS). Because of their distinctive properties, FANS may play a unique role in striatal information processing.https://www.frontiersin.org/article/10.3389/fncir.2019.00032/fullinterneuronsneuron diversitydorsal striatumtyrosine hydroxylaseintrinsic electrical propertiesneuron classification |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Miaomiao Mao Miaomiao Mao Aditya Nair Aditya Nair Aditya Nair Aditya Nair George J. Augustine George J. Augustine |
spellingShingle |
Miaomiao Mao Miaomiao Mao Aditya Nair Aditya Nair Aditya Nair Aditya Nair George J. Augustine George J. Augustine A Novel Type of Neuron Within the Dorsal Striatum Frontiers in Neural Circuits interneurons neuron diversity dorsal striatum tyrosine hydroxylase intrinsic electrical properties neuron classification |
author_facet |
Miaomiao Mao Miaomiao Mao Aditya Nair Aditya Nair Aditya Nair Aditya Nair George J. Augustine George J. Augustine |
author_sort |
Miaomiao Mao |
title |
A Novel Type of Neuron Within the Dorsal Striatum |
title_short |
A Novel Type of Neuron Within the Dorsal Striatum |
title_full |
A Novel Type of Neuron Within the Dorsal Striatum |
title_fullStr |
A Novel Type of Neuron Within the Dorsal Striatum |
title_full_unstemmed |
A Novel Type of Neuron Within the Dorsal Striatum |
title_sort |
novel type of neuron within the dorsal striatum |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Neural Circuits |
issn |
1662-5110 |
publishDate |
2019-05-01 |
description |
The striatum is predominantly composed of medium spiny projection neurons, with the remaining neurons consisting of several types of interneurons. Among the interneurons are a group of cells that express tyrosine hydroxylase (TH). Although the intrinsic electrical properties of these TH-expressing interneurons have been characterized, there is no agreement on the number of TH-expressing cell types and their electrical properties. Here, we have used transgenic mice in which YFP-tagged channelrhodopsin-2 (ChR2) was expressed in potential TH-expressing cells in a Cre-dependent manner. We found that the YFP+ neurons in the striatum were heterogeneous in their intrinsic electrical properties; unbiased clustering indicated that there are three main neuronal subtypes. One population of neurons had aspiny dendrites with high-frequency action potential (AP) firing and plateau potentials, resembling the TH interneurons (THIN) described previously. A second, very small population of labeled neurons resembled medium-sized spiny neurons (MSN). The third population of neurons had dendrites with an intermediate density of spines, showed substantial AP adaptation and generated prolonged spikes. This type of striatal neuron has not been previously identified in the adult mouse and we have named it the Frequency-Adapting Neuron with Spines (FANS). Because of their distinctive properties, FANS may play a unique role in striatal information processing. |
topic |
interneurons neuron diversity dorsal striatum tyrosine hydroxylase intrinsic electrical properties neuron classification |
url |
https://www.frontiersin.org/article/10.3389/fncir.2019.00032/full |
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