African relapsing fever borreliae genomospecies revealed by comparative genomics
Background:<br/>Relapsing fever borreliae are vector-borne bacteria responsible for febrile infection in humans in North America, Africa, Asia and in the Iberian Peninsula in Europe. Relapsing fever borreliae are phylogenetically closely related, yet they differ in pathogenicity and vectors. T...
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doaj-89d44cba0afb40f7b77f96e800c631692020-11-24T20:45:12ZengFrontiers Media S.A.Frontiers in Public Health2296-25652014-05-01210.3389/fpubh.2014.0004388982African relapsing fever borreliae genomospecies revealed by comparative genomicsHaitham eElbir0Laurent eAbi-Rached1Pierre ePontarotti2Niayz eYoosuf3Michel eDRANCOURT4Aix Marseille UniversitéAix Marseille UniversitéAix Marseille UniversitéAix Marseille UniversitéAix Marseille UniversitéBackground:<br/>Relapsing fever borreliae are vector-borne bacteria responsible for febrile infection in humans in North America, Africa, Asia and in the Iberian Peninsula in Europe. Relapsing fever borreliae are phylogenetically closely related, yet they differ in pathogenicity and vectors. Their long-term taxonomy, based on geography and vector grouping, needs a re-appraisal in the genomic area. We therefore embarked into genomic analyses of relapsing fever borreliae, focusing on species found in Africa. <br/><br/>Results:<br/>Genome-wide phylogenetic analyses group Old World Borrelia crocidurae, Borrelia hispanica, B. duttonii and B. recurrentis in one clade, and New World Borrelia turicatae and Borrelia hermsii in a second clade. Accordingly, average nucleotide identity is 99% among B. duttonii, B. recurrentis and B. crocidurae and 96% between latter borreliae and B. hispanica while the similarity is 86% between Old World and New World borreliae. Comparative genomics indicates that the Old World relapsing fever B. duttonii, B. recurrentis, B. crocidurae and B. hispanica have a 2,514-gene pan-genome and a 933-gene core genome that includes 788 chromosomal and 145 plasmidic genes. Analysing the role that natural selection has played in the evolution of Old World borreliae species revealed that 55 loci were under positive diversifying selection, including loci coding for membrane, flagellar and chemotaxis proteins, three categories associated with adaption to specific niches. <br/> <br/>Conclusions:<br/>Genomic analyses led to a reappraisal of the taxonomy of relapsing fever borreliae in Africa. These analyses suggest that B. crocidurae, B. duttonii and B. recurrentis are ecotypes of a unique genomospecies, while B. hispanica is a distinct species. <br/>http://journal.frontiersin.org/Journal/10.3389/fpubh.2014.00043/fullBorreliaGenomicsRelapsing Feverphylogenyspeciation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Haitham eElbir Laurent eAbi-Rached Pierre ePontarotti Niayz eYoosuf Michel eDRANCOURT |
spellingShingle |
Haitham eElbir Laurent eAbi-Rached Pierre ePontarotti Niayz eYoosuf Michel eDRANCOURT African relapsing fever borreliae genomospecies revealed by comparative genomics Frontiers in Public Health Borrelia Genomics Relapsing Fever phylogeny speciation |
author_facet |
Haitham eElbir Laurent eAbi-Rached Pierre ePontarotti Niayz eYoosuf Michel eDRANCOURT |
author_sort |
Haitham eElbir |
title |
African relapsing fever borreliae genomospecies revealed by comparative genomics |
title_short |
African relapsing fever borreliae genomospecies revealed by comparative genomics |
title_full |
African relapsing fever borreliae genomospecies revealed by comparative genomics |
title_fullStr |
African relapsing fever borreliae genomospecies revealed by comparative genomics |
title_full_unstemmed |
African relapsing fever borreliae genomospecies revealed by comparative genomics |
title_sort |
african relapsing fever borreliae genomospecies revealed by comparative genomics |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Public Health |
issn |
2296-2565 |
publishDate |
2014-05-01 |
description |
Background:<br/>Relapsing fever borreliae are vector-borne bacteria responsible for febrile infection in humans in North America, Africa, Asia and in the Iberian Peninsula in Europe. Relapsing fever borreliae are phylogenetically closely related, yet they differ in pathogenicity and vectors. Their long-term taxonomy, based on geography and vector grouping, needs a re-appraisal in the genomic area. We therefore embarked into genomic analyses of relapsing fever borreliae, focusing on species found in Africa. <br/><br/>Results:<br/>Genome-wide phylogenetic analyses group Old World Borrelia crocidurae, Borrelia hispanica, B. duttonii and B. recurrentis in one clade, and New World Borrelia turicatae and Borrelia hermsii in a second clade. Accordingly, average nucleotide identity is 99% among B. duttonii, B. recurrentis and B. crocidurae and 96% between latter borreliae and B. hispanica while the similarity is 86% between Old World and New World borreliae. Comparative genomics indicates that the Old World relapsing fever B. duttonii, B. recurrentis, B. crocidurae and B. hispanica have a 2,514-gene pan-genome and a 933-gene core genome that includes 788 chromosomal and 145 plasmidic genes. Analysing the role that natural selection has played in the evolution of Old World borreliae species revealed that 55 loci were under positive diversifying selection, including loci coding for membrane, flagellar and chemotaxis proteins, three categories associated with adaption to specific niches. <br/> <br/>Conclusions:<br/>Genomic analyses led to a reappraisal of the taxonomy of relapsing fever borreliae in Africa. These analyses suggest that B. crocidurae, B. duttonii and B. recurrentis are ecotypes of a unique genomospecies, while B. hispanica is a distinct species. <br/> |
topic |
Borrelia Genomics Relapsing Fever phylogeny speciation |
url |
http://journal.frontiersin.org/Journal/10.3389/fpubh.2014.00043/full |
work_keys_str_mv |
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