Real-Time Whole-Genome Sequencing for Surveillance of Listeria monocytogenes, France
During 2015–2016, we evaluated the performance of whole-genome sequencing (WGS) as a routine typing tool. Its added value for microbiological and epidemiologic surveillance of listeriosis was compared with that for pulsed-field gel electrophoresis (PFGE), the current standard method. A total of 2,74...
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doaj-89e1de26d8354a3195f41333e8a5ac612020-11-25T00:32:12ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592017-09-012391462147010.3201/eid2309.170336Real-Time Whole-Genome Sequencing for Surveillance of Listeria monocytogenes, FranceAlexandra MouraMathieu TourdjmanAlexandre LeclercqEstelle HamelinEdith LaurentNathalie FredriksenDieter Van CauterenHélène Bracq-DieyePierre ThouvenotGuillaume ValesNathalie Tessaud-RitaMylène M. MauryAndreea AlexandruAlexis CriscuoloEmmanuel QuevillonMarie-Pierre DonguyVincent EnoufHenriette de ValkSylvain BrisseMarc LecuitDuring 2015–2016, we evaluated the performance of whole-genome sequencing (WGS) as a routine typing tool. Its added value for microbiological and epidemiologic surveillance of listeriosis was compared with that for pulsed-field gel electrophoresis (PFGE), the current standard method. A total of 2,743 Listeria monocytogenes isolates collected as part of routine surveillance were characterized in parallel by PFGE and core genome multilocus sequence typing (cgMLST) extracted from WGS. We investigated PFGE and cgMLST clusters containing human isolates. Discrimination of isolates was significantly higher by cgMLST than by PFGE (p<0.001). cgMLST discriminated unrelated isolates that shared identical PFGE profiles and phylogenetically closely related isolates with distinct PFGE profiles. This procedure also refined epidemiologic investigations to include only phylogenetically closely related isolates, improved source identification, and facilitated epidemiologic investigations, enabling identification of more outbreaks at earlier stages. WGS-based typing should replace PFGE as the primary typing method for L. monocytogenes.https://wwwnc.cdc.gov/eid/article/23/9/17-0336_articleListeria monocytogenesbacteriawhole-genome sequencinghigh-throughput DNA sequencingmolecular typingpublic health |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alexandra Moura Mathieu Tourdjman Alexandre Leclercq Estelle Hamelin Edith Laurent Nathalie Fredriksen Dieter Van Cauteren Hélène Bracq-Dieye Pierre Thouvenot Guillaume Vales Nathalie Tessaud-Rita Mylène M. Maury Andreea Alexandru Alexis Criscuolo Emmanuel Quevillon Marie-Pierre Donguy Vincent Enouf Henriette de Valk Sylvain Brisse Marc Lecuit |
spellingShingle |
Alexandra Moura Mathieu Tourdjman Alexandre Leclercq Estelle Hamelin Edith Laurent Nathalie Fredriksen Dieter Van Cauteren Hélène Bracq-Dieye Pierre Thouvenot Guillaume Vales Nathalie Tessaud-Rita Mylène M. Maury Andreea Alexandru Alexis Criscuolo Emmanuel Quevillon Marie-Pierre Donguy Vincent Enouf Henriette de Valk Sylvain Brisse Marc Lecuit Real-Time Whole-Genome Sequencing for Surveillance of Listeria monocytogenes, France Emerging Infectious Diseases Listeria monocytogenes bacteria whole-genome sequencing high-throughput DNA sequencing molecular typing public health |
author_facet |
Alexandra Moura Mathieu Tourdjman Alexandre Leclercq Estelle Hamelin Edith Laurent Nathalie Fredriksen Dieter Van Cauteren Hélène Bracq-Dieye Pierre Thouvenot Guillaume Vales Nathalie Tessaud-Rita Mylène M. Maury Andreea Alexandru Alexis Criscuolo Emmanuel Quevillon Marie-Pierre Donguy Vincent Enouf Henriette de Valk Sylvain Brisse Marc Lecuit |
author_sort |
Alexandra Moura |
title |
Real-Time Whole-Genome Sequencing for Surveillance of Listeria monocytogenes, France |
title_short |
Real-Time Whole-Genome Sequencing for Surveillance of Listeria monocytogenes, France |
title_full |
Real-Time Whole-Genome Sequencing for Surveillance of Listeria monocytogenes, France |
title_fullStr |
Real-Time Whole-Genome Sequencing for Surveillance of Listeria monocytogenes, France |
title_full_unstemmed |
Real-Time Whole-Genome Sequencing for Surveillance of Listeria monocytogenes, France |
title_sort |
real-time whole-genome sequencing for surveillance of listeria monocytogenes, france |
publisher |
Centers for Disease Control and Prevention |
series |
Emerging Infectious Diseases |
issn |
1080-6040 1080-6059 |
publishDate |
2017-09-01 |
description |
During 2015–2016, we evaluated the performance of whole-genome sequencing (WGS) as a routine typing tool. Its added value for microbiological and epidemiologic surveillance of listeriosis was compared with that for pulsed-field gel electrophoresis (PFGE), the current standard method. A total of 2,743 Listeria monocytogenes isolates collected as part of routine surveillance were characterized in parallel by PFGE and core genome multilocus sequence typing (cgMLST) extracted from WGS. We investigated PFGE and cgMLST clusters containing human isolates. Discrimination of isolates was significantly higher by cgMLST than by PFGE (p<0.001). cgMLST discriminated unrelated isolates that shared identical PFGE profiles and phylogenetically closely related isolates with distinct PFGE profiles. This procedure also refined epidemiologic investigations to include only phylogenetically closely related isolates, improved source identification, and facilitated epidemiologic investigations, enabling identification of more outbreaks at earlier stages. WGS-based typing should replace PFGE as the primary typing method for L. monocytogenes. |
topic |
Listeria monocytogenes bacteria whole-genome sequencing high-throughput DNA sequencing molecular typing public health |
url |
https://wwwnc.cdc.gov/eid/article/23/9/17-0336_article |
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