Naringenin Regulates CFTR Activation and Expression in Airway Epithelial Cells
Background/Aims: Sputum symptoms are commonly seen in the elderly. This study aimed to identify an efficacious expectorant treatment stratagem through evaluating the secretion-promoting activation and cystic fibrosis transmembrane conductance regulator (CFTR) expression of the bioactive herbal monom...
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Cell Physiol Biochem Press GmbH & Co KG
2017-11-01
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doaj-89eb06b796314c5d93530d6788e484592020-11-25T02:19:31ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782017-11-014431146116010.1159/000485419485419Naringenin Regulates CFTR Activation and Expression in Airway Epithelial CellsRui ShiZi-Ting XiaoYi-Jun ZhengYi-Lin ZhangJia-Wen XuJie-Hong HuangWen-Liang ZhouPei-Bo LiWei-Wei SuBackground/Aims: Sputum symptoms are commonly seen in the elderly. This study aimed to identify an efficacious expectorant treatment stratagem through evaluating the secretion-promoting activation and cystic fibrosis transmembrane conductance regulator (CFTR) expression of the bioactive herbal monomer naringenin. Methods: Vectorial Cl- transport was determined by measuring short-circuit current (ISC) in rat airway epithelium. cAMP content was measured by ELISA in primary cultured epithelial cells and Calu-3 cells. CFTR expression in Calu-3 cells was determined by qPCR. Results: Addition of naringenin to the basolateral side of the rat airway led to a concentration-dependent sustained increase in ISC. The current was suppressed when exposed to Cl–-free solution or by bumetanide, BaCl2, and DPC but not by DIDS and IBMX. Forskolin-induced ISC increase and CFTRinh-172/MDL-12330A-induced ISC inhibition were not altered by naringenin. Intracellular cAMP content was significantly increased by naringenin. With lipopolysaccharide stimulation, CFTR expression was significantly reduced, and naringenin dose-dependently enhanced CFTR mRNA expression. Conclusion: These results demonstrate that naringenin has the ability to stimulate Cl- secretion, which is mediated by CFTR through a signaling pathway by increasing cAMP content. Moreover, naringenin can increase CFTR expression when organism CFTR expression is seriously hampered. Our data suggest a potentially effective treatment strategy for sputum.https://www.karger.com/Article/FullText/485419NaringeninAirway epitheliumCFTRCl- secretionTraditional Chinese medicine |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rui Shi Zi-Ting Xiao Yi-Jun Zheng Yi-Lin Zhang Jia-Wen Xu Jie-Hong Huang Wen-Liang Zhou Pei-Bo Li Wei-Wei Su |
spellingShingle |
Rui Shi Zi-Ting Xiao Yi-Jun Zheng Yi-Lin Zhang Jia-Wen Xu Jie-Hong Huang Wen-Liang Zhou Pei-Bo Li Wei-Wei Su Naringenin Regulates CFTR Activation and Expression in Airway Epithelial Cells Cellular Physiology and Biochemistry Naringenin Airway epithelium CFTR Cl- secretion Traditional Chinese medicine |
author_facet |
Rui Shi Zi-Ting Xiao Yi-Jun Zheng Yi-Lin Zhang Jia-Wen Xu Jie-Hong Huang Wen-Liang Zhou Pei-Bo Li Wei-Wei Su |
author_sort |
Rui Shi |
title |
Naringenin Regulates CFTR Activation and Expression in Airway Epithelial Cells |
title_short |
Naringenin Regulates CFTR Activation and Expression in Airway Epithelial Cells |
title_full |
Naringenin Regulates CFTR Activation and Expression in Airway Epithelial Cells |
title_fullStr |
Naringenin Regulates CFTR Activation and Expression in Airway Epithelial Cells |
title_full_unstemmed |
Naringenin Regulates CFTR Activation and Expression in Airway Epithelial Cells |
title_sort |
naringenin regulates cftr activation and expression in airway epithelial cells |
publisher |
Cell Physiol Biochem Press GmbH & Co KG |
series |
Cellular Physiology and Biochemistry |
issn |
1015-8987 1421-9778 |
publishDate |
2017-11-01 |
description |
Background/Aims: Sputum symptoms are commonly seen in the elderly. This study aimed to identify an efficacious expectorant treatment stratagem through evaluating the secretion-promoting activation and cystic fibrosis transmembrane conductance regulator (CFTR) expression of the bioactive herbal monomer naringenin. Methods: Vectorial Cl- transport was determined by measuring short-circuit current (ISC) in rat airway epithelium. cAMP content was measured by ELISA in primary cultured epithelial cells and Calu-3 cells. CFTR expression in Calu-3 cells was determined by qPCR. Results: Addition of naringenin to the basolateral side of the rat airway led to a concentration-dependent sustained increase in ISC. The current was suppressed when exposed to Cl–-free solution or by bumetanide, BaCl2, and DPC but not by DIDS and IBMX. Forskolin-induced ISC increase and CFTRinh-172/MDL-12330A-induced ISC inhibition were not altered by naringenin. Intracellular cAMP content was significantly increased by naringenin. With lipopolysaccharide stimulation, CFTR expression was significantly reduced, and naringenin dose-dependently enhanced CFTR mRNA expression. Conclusion: These results demonstrate that naringenin has the ability to stimulate Cl- secretion, which is mediated by CFTR through a signaling pathway by increasing cAMP content. Moreover, naringenin can increase CFTR expression when organism CFTR expression is seriously hampered. Our data suggest a potentially effective treatment strategy for sputum. |
topic |
Naringenin Airway epithelium CFTR Cl- secretion Traditional Chinese medicine |
url |
https://www.karger.com/Article/FullText/485419 |
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