The Association of Vitamin D Receptor Polymorphisms of FokI and TaqI with Rheumatoid Arthritis in North-East of Iran
Background and objectives: Vitamin D receptor (VDR) has been identified as a susceptibility gene for several autoimmune diseases. This study was designed to investigate the association of VDR gene polymorphisms with the susceptibility to rheumatoid arthritis (RA). Methods: A case-control study was p...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Golestan University of Medical Sciences
2019-12-01
|
Series: | Jorjani Biomedicine Journal |
Subjects: | |
Online Access: | http://goums.ac.ir/jorjanijournal/article-1-705-en.html |
id |
doaj-89f6e5a3c531470087f546be40152101 |
---|---|
record_format |
Article |
spelling |
doaj-89f6e5a3c531470087f546be401521012020-11-25T03:23:47ZengGolestan University of Medical Sciences Jorjani Biomedicine Journal2645-35092019-12-01742029The Association of Vitamin D Receptor Polymorphisms of FokI and TaqI with Rheumatoid Arthritis in North-East of Irannafiseh kaviani0yaghoub yazdani1Hadi Bazzazi2 Department of Biology, Gorgan Branch, Islamic Azad University, Gorgan, Iran Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, Iran Department of Medical Laboratory Sciences, Gorgan Branch, Islamic Azad University, Gorgan, Iran Background and objectives: Vitamin D receptor (VDR) has been identified as a susceptibility gene for several autoimmune diseases. This study was designed to investigate the association of VDR gene polymorphisms with the susceptibility to rheumatoid arthritis (RA). Methods: A case-control study was performed on 130 RA patients and 128 healthy subjects in the north-east of Iran using restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) technique. Results: Our findings suggested a significant association of T allele (p=0.01) of TaqI (rs731236), and f allele (p=0.01) of FokI (rs10735810) genetic variants of the VDR gene with RA susceptibility. These significant associations were also found in the T/T genotype of TaqI (p=0.009), and F/f genotype of FokI (P=0.014). The f-T haplotype was more significantly detected in-patients than in healthy controls (p=0.007). Conclusion: The RA group showed an increase in the f allele and heterozygous F/f genotype and also in the T allele and homozygous T/T and heterozygous T/t genotypes as compared to the control group. Our results demonstrated that polymorphisms of TaqI and FokI in the VDR gene might be involved in the development of RA in an Iranian population.http://goums.ac.ir/jorjanijournal/article-1-705-en.htmlfokitaqivitamin d receptorrheumatoid arthritisrestriction fragment length polymorphism-polymerase chain reaction (rflp-pcr) |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
nafiseh kaviani yaghoub yazdani Hadi Bazzazi |
spellingShingle |
nafiseh kaviani yaghoub yazdani Hadi Bazzazi The Association of Vitamin D Receptor Polymorphisms of FokI and TaqI with Rheumatoid Arthritis in North-East of Iran Jorjani Biomedicine Journal foki taqi vitamin d receptor rheumatoid arthritis restriction fragment length polymorphism-polymerase chain reaction (rflp-pcr) |
author_facet |
nafiseh kaviani yaghoub yazdani Hadi Bazzazi |
author_sort |
nafiseh kaviani |
title |
The Association of Vitamin D Receptor Polymorphisms of FokI and TaqI with Rheumatoid Arthritis in North-East of Iran |
title_short |
The Association of Vitamin D Receptor Polymorphisms of FokI and TaqI with Rheumatoid Arthritis in North-East of Iran |
title_full |
The Association of Vitamin D Receptor Polymorphisms of FokI and TaqI with Rheumatoid Arthritis in North-East of Iran |
title_fullStr |
The Association of Vitamin D Receptor Polymorphisms of FokI and TaqI with Rheumatoid Arthritis in North-East of Iran |
title_full_unstemmed |
The Association of Vitamin D Receptor Polymorphisms of FokI and TaqI with Rheumatoid Arthritis in North-East of Iran |
title_sort |
association of vitamin d receptor polymorphisms of foki and taqi with rheumatoid arthritis in north-east of iran |
publisher |
Golestan University of Medical Sciences |
series |
Jorjani Biomedicine Journal |
issn |
2645-3509 |
publishDate |
2019-12-01 |
description |
Background and objectives: Vitamin D receptor (VDR) has been identified as a susceptibility gene for several autoimmune diseases. This study was designed to investigate the association of VDR gene polymorphisms with the susceptibility to rheumatoid arthritis (RA).
Methods: A case-control study was performed on 130 RA patients and 128 healthy subjects in the north-east of Iran using restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) technique.
Results: Our findings suggested a significant association of T allele (p=0.01) of TaqI (rs731236), and f allele (p=0.01) of FokI (rs10735810) genetic variants of the VDR gene with RA susceptibility. These significant associations were also found in the T/T genotype of TaqI (p=0.009), and F/f genotype of FokI (P=0.014). The f-T haplotype was more significantly detected in-patients than in healthy controls (p=0.007).
Conclusion: The RA group showed an increase in the f allele and heterozygous F/f genotype and also in the T allele and homozygous T/T and heterozygous T/t genotypes as compared to the control group. Our results demonstrated that polymorphisms of TaqI and FokI in the VDR gene might be involved in the development of RA in an Iranian population. |
topic |
foki taqi vitamin d receptor rheumatoid arthritis restriction fragment length polymorphism-polymerase chain reaction (rflp-pcr) |
url |
http://goums.ac.ir/jorjanijournal/article-1-705-en.html |
work_keys_str_mv |
AT nafisehkaviani theassociationofvitamindreceptorpolymorphismsoffokiandtaqiwithrheumatoidarthritisinnortheastofiran AT yaghoubyazdani theassociationofvitamindreceptorpolymorphismsoffokiandtaqiwithrheumatoidarthritisinnortheastofiran AT hadibazzazi theassociationofvitamindreceptorpolymorphismsoffokiandtaqiwithrheumatoidarthritisinnortheastofiran AT nafisehkaviani associationofvitamindreceptorpolymorphismsoffokiandtaqiwithrheumatoidarthritisinnortheastofiran AT yaghoubyazdani associationofvitamindreceptorpolymorphismsoffokiandtaqiwithrheumatoidarthritisinnortheastofiran AT hadibazzazi associationofvitamindreceptorpolymorphismsoffokiandtaqiwithrheumatoidarthritisinnortheastofiran |
_version_ |
1724604591288352768 |