Differentiation between Acute Skin Rejection in Allotransplantation and T-Cell Mediated Skin Inflammation Based on Gene Expression Analysis
Advances in microsurgical techniques and immunosuppressive medication have rendered transplantation of vascularized composite allografts possible, when autologous tissue is neither available nor sufficient for reconstruction. However, skin rejection and side effects of long-term immunosuppression st...
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doaj-89fd87a114a64ca99c373f0ac2b22b9b2020-11-25T00:29:58ZengHindawi LimitedBioMed Research International2314-61332314-61412015-01-01201510.1155/2015/259160259160Differentiation between Acute Skin Rejection in Allotransplantation and T-Cell Mediated Skin Inflammation Based on Gene Expression AnalysisDolores Wolfram0Evi M. Morandi1Nadine Eberhart2Theresa Hautz3Hubert Hackl4Bettina Zelger5Gregor Riede6Tanja Wachter7Sandrine Dubrac8Christian Ploner9Gerhard Pierer10Stefan Schneeberger11Department of Plastic, Reconstructive and Aesthetic Surgery, Medical University of Innsbruck, Anichstrasse 35, 6020 Innsbruck, AustriaDepartment of Plastic, Reconstructive and Aesthetic Surgery, Medical University of Innsbruck, Anichstrasse 35, 6020 Innsbruck, AustriaDepartment of Plastic, Reconstructive and Aesthetic Surgery, Medical University of Innsbruck, Anichstrasse 35, 6020 Innsbruck, AustriaDepartment of Visceral, Transplant and Thoracic Surgery, Medical University of Innsbruck, Anichstrasse 35, 6020 Innsbruck, AustriaDivision of Bioinformatics, Biocenter, Medical University of Innsbruck, Innrain 80, 6020 Innsbruck, AustriaDepartment of Pathology, Medical University of Innsbruck, Muellerstrasse 44, 6020 Innsbruck, AustriaDepartment of Plastic, Reconstructive and Aesthetic Surgery, Medical University of Innsbruck, Anichstrasse 35, 6020 Innsbruck, AustriaDepartment of Plastic, Reconstructive and Aesthetic Surgery, Medical University of Innsbruck, Anichstrasse 35, 6020 Innsbruck, AustriaDepartment of Dermatology and Venereology, Medical University of Innsbruck, Anichstrasse 35, 6020 Innsbruck, AustriaDepartment of Plastic, Reconstructive and Aesthetic Surgery, Medical University of Innsbruck, Anichstrasse 35, 6020 Innsbruck, AustriaDepartment of Plastic, Reconstructive and Aesthetic Surgery, Medical University of Innsbruck, Anichstrasse 35, 6020 Innsbruck, AustriaDepartment of Visceral, Transplant and Thoracic Surgery, Medical University of Innsbruck, Anichstrasse 35, 6020 Innsbruck, AustriaAdvances in microsurgical techniques and immunosuppressive medication have rendered transplantation of vascularized composite allografts possible, when autologous tissue is neither available nor sufficient for reconstruction. However, skin rejection and side effects of long-term immunosuppression still remain a major hurdle for wide adoption of this excellent reconstructive technique. Histopathologic changes during acute skin rejection in vascular composite allotransplantation often mimic inflammatory skin disorders and are hard to distinguish. Hence, the identification of diagnostic and therapeutic markers specific for skin rejection is of particular clinical need. Here we present novel markers allowing for early differentiation between rejection in hind limb allotransplantation and contact hypersensitivity. Assessment of Ccl7, Il18, and Il1b expression is most indicative of distinguishing skin rejection from skin inflammatory disorders. Gene expression levels varied significantly across skin types and regions, indicating localization specific mechanism of leukocyte migration and infiltration. Expression of Il12b, Il17a, and Il1b gene expression levels differed significantly between rejection and inflammation, independent of the skin type. In synopsis of the RNA expression profile and previously assessed protein expression, the Il1 family appears as a promising option for accurate skin rejection diagnosis and, as a following step, for development of novel rejection treatments.http://dx.doi.org/10.1155/2015/259160 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Dolores Wolfram Evi M. Morandi Nadine Eberhart Theresa Hautz Hubert Hackl Bettina Zelger Gregor Riede Tanja Wachter Sandrine Dubrac Christian Ploner Gerhard Pierer Stefan Schneeberger |
spellingShingle |
Dolores Wolfram Evi M. Morandi Nadine Eberhart Theresa Hautz Hubert Hackl Bettina Zelger Gregor Riede Tanja Wachter Sandrine Dubrac Christian Ploner Gerhard Pierer Stefan Schneeberger Differentiation between Acute Skin Rejection in Allotransplantation and T-Cell Mediated Skin Inflammation Based on Gene Expression Analysis BioMed Research International |
author_facet |
Dolores Wolfram Evi M. Morandi Nadine Eberhart Theresa Hautz Hubert Hackl Bettina Zelger Gregor Riede Tanja Wachter Sandrine Dubrac Christian Ploner Gerhard Pierer Stefan Schneeberger |
author_sort |
Dolores Wolfram |
title |
Differentiation between Acute Skin Rejection in Allotransplantation and T-Cell Mediated Skin Inflammation Based on Gene Expression Analysis |
title_short |
Differentiation between Acute Skin Rejection in Allotransplantation and T-Cell Mediated Skin Inflammation Based on Gene Expression Analysis |
title_full |
Differentiation between Acute Skin Rejection in Allotransplantation and T-Cell Mediated Skin Inflammation Based on Gene Expression Analysis |
title_fullStr |
Differentiation between Acute Skin Rejection in Allotransplantation and T-Cell Mediated Skin Inflammation Based on Gene Expression Analysis |
title_full_unstemmed |
Differentiation between Acute Skin Rejection in Allotransplantation and T-Cell Mediated Skin Inflammation Based on Gene Expression Analysis |
title_sort |
differentiation between acute skin rejection in allotransplantation and t-cell mediated skin inflammation based on gene expression analysis |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2015-01-01 |
description |
Advances in microsurgical techniques and immunosuppressive medication have rendered transplantation of vascularized composite allografts possible, when autologous tissue is neither available nor sufficient for reconstruction. However, skin rejection and side effects of long-term immunosuppression still remain a major hurdle for wide adoption of this excellent reconstructive technique. Histopathologic changes during acute skin rejection in vascular composite allotransplantation often mimic inflammatory skin disorders and are hard to distinguish. Hence, the identification of diagnostic and therapeutic markers specific for skin rejection is of particular clinical need. Here we present novel markers allowing for early differentiation between rejection in hind limb allotransplantation and contact hypersensitivity. Assessment of Ccl7, Il18, and Il1b expression is most indicative of distinguishing skin rejection from skin inflammatory disorders. Gene expression levels varied significantly across skin types and regions, indicating localization specific mechanism of leukocyte migration and infiltration. Expression of Il12b, Il17a, and Il1b gene expression levels differed significantly between rejection and inflammation, independent of the skin type. In synopsis of the RNA expression profile and previously assessed protein expression, the Il1 family appears as a promising option for accurate skin rejection diagnosis and, as a following step, for development of novel rejection treatments. |
url |
http://dx.doi.org/10.1155/2015/259160 |
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