DNA methylation alteration is a major consequence of genome doubling in autotetraploid Brassica rapa
Polyploids are typically classified as autopolyploids or allopolyploids based on the origin of their chromosome sets. Autopolyploidy is much more common than traditionally believed. Allopolyploidization, accompanied by genomic and transcriptomic changes, has been well investigated. In this...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
University of Belgrade, University of Novi Sad
2017-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2017/0354-46641700015X.pdf |
| Summary: | Polyploids are typically classified as autopolyploids or allopolyploids based
on the origin of their chromosome sets. Autopolyploidy is much more common
than traditionally believed. Allopolyploidization, accompanied by genomic
and transcriptomic changes, has been well investigated. In this study,
genetic, DNA methylation and gene expression changes in autotetraploid
Brassica rapa were investigated. No genetic alteration was detected using an
amplified fragment length polymorphism (AFLP) approach. Using a cDNA-AFLP
approach, approximately 0.58% of fragments showed changes in gene expression
in autotetraploid B. rapa. The methylation-sensitive amplification
polymorphism (MSAP) analysis showed that approximately 1.7% of the fragments
underwent DNA methylation changes upon genome doubling, with
hypermethylation and demethylation changes equally affected. Fragments
displaying changes in gene expression and methylation status were isolated
and then sequenced and characterized, respectively. This study showed that
variation in cytosine methylation is a major consequence of genome doubling
in autotetraploid Brassica rapa. |
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| ISSN: | 0354-4664 1821-4339 |