Evaluation of the potential defensive strategy against Influenza A in cell line models [version 2; referees: 2 approved]
Background: Influenza virus can cause both seasonal infections and unpredictable pandemics. Rapidly evolving avian H5N1 and H7N9 viruses have a potential pandemic threat for humans. Since avian Influenza can be transmitted by domestic birds, serving as a key link between wild birds and humans, an e...
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doaj-8a2c8beaaefc4653b74a4c2b6a6029962020-11-25T03:04:44ZengF1000 Research LtdF1000Research2046-14022018-05-01710.12688/f1000research.13496.216185Evaluation of the potential defensive strategy against Influenza A in cell line models [version 2; referees: 2 approved]Ekaterina Antonova0Olga Glazova1Anna Gaponova2Aykaz Eremyan3Natalya Grebenkina4Svetlana Zvereva5Natalya Volkova6Pavel Volchkov7Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, 141701, Russian FederationMoscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, 141701, Russian FederationMoscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, 141701, Russian FederationMoscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, 141701, Russian FederationMoscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, 141701, Russian FederationMoscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, 141701, Russian FederationErnst Institute of Animal Husbandry, Podolsk Municipal District, Moscow Region, 142132, Russian FederationMoscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, 141701, Russian FederationBackground: Influenza virus can cause both seasonal infections and unpredictable pandemics. Rapidly evolving avian H5N1 and H7N9 viruses have a potential pandemic threat for humans. Since avian Influenza can be transmitted by domestic birds, serving as a key link between wild birds and humans, an effective measure to control the influenza transmission would be eradication of the infection in poultry. It is known that the virus penetrates into the cell through binding with the terminal oligosaccharides - sialic acids (SA) - on the cell surfaces. Removal of SA might be a potential antiviral strategy. An approach to developing chicken lines that are resistant to influenza viruses could be the creation of genetically modified birds. Thus it is necessary to select a gene that provides defense to influenza. Here we have expressed in cells a range of exogenous sialidases and estimated their activity and specificity towards SA residues. Methods: Several bacterial, viral and human sialidases were tested. We adopted bacterial sialidases from Salmonella and Actinomyces for expression on the cell surface by fusing catalytic domains with transmembrane domains. We also selected Influenza A/PuertoRico/8/34/H1N1 neuraminidase and human membrane sialidase ( hNeu3) genes. Lectin binding assay was used for estimation of a α (2,3)-sialylation level by fluorescent microscopy and FACS. Results: We compared sialidases from bacteria, Influenza virus and human. Sialidases from Salmonella and Influenza A neuraminidase effectively cleaved α (2-3)-SA receptors. Viral neuraminidase demonstrated a higher activity. Sialidases from Actinomyces and hNeu3 did not show any activity against α (2-3) SA under physiological conditions. Conclusion: Our results demonstrated that sialidases with different specificity and activity can be selected as genes providing antiviral defence. Combining chosen sialidases with different activity together with tissue-specific promoters would provide an optimal level of desialylation. Tissue specific expression of the sialidases could protect domestic birds from infection.https://f1000research.com/articles/7-206/v2 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ekaterina Antonova Olga Glazova Anna Gaponova Aykaz Eremyan Natalya Grebenkina Svetlana Zvereva Natalya Volkova Pavel Volchkov |
spellingShingle |
Ekaterina Antonova Olga Glazova Anna Gaponova Aykaz Eremyan Natalya Grebenkina Svetlana Zvereva Natalya Volkova Pavel Volchkov Evaluation of the potential defensive strategy against Influenza A in cell line models [version 2; referees: 2 approved] F1000Research |
author_facet |
Ekaterina Antonova Olga Glazova Anna Gaponova Aykaz Eremyan Natalya Grebenkina Svetlana Zvereva Natalya Volkova Pavel Volchkov |
author_sort |
Ekaterina Antonova |
title |
Evaluation of the potential defensive strategy against Influenza A in cell line models [version 2; referees: 2 approved] |
title_short |
Evaluation of the potential defensive strategy against Influenza A in cell line models [version 2; referees: 2 approved] |
title_full |
Evaluation of the potential defensive strategy against Influenza A in cell line models [version 2; referees: 2 approved] |
title_fullStr |
Evaluation of the potential defensive strategy against Influenza A in cell line models [version 2; referees: 2 approved] |
title_full_unstemmed |
Evaluation of the potential defensive strategy against Influenza A in cell line models [version 2; referees: 2 approved] |
title_sort |
evaluation of the potential defensive strategy against influenza a in cell line models [version 2; referees: 2 approved] |
publisher |
F1000 Research Ltd |
series |
F1000Research |
issn |
2046-1402 |
publishDate |
2018-05-01 |
description |
Background: Influenza virus can cause both seasonal infections and unpredictable pandemics. Rapidly evolving avian H5N1 and H7N9 viruses have a potential pandemic threat for humans. Since avian Influenza can be transmitted by domestic birds, serving as a key link between wild birds and humans, an effective measure to control the influenza transmission would be eradication of the infection in poultry. It is known that the virus penetrates into the cell through binding with the terminal oligosaccharides - sialic acids (SA) - on the cell surfaces. Removal of SA might be a potential antiviral strategy. An approach to developing chicken lines that are resistant to influenza viruses could be the creation of genetically modified birds. Thus it is necessary to select a gene that provides defense to influenza. Here we have expressed in cells a range of exogenous sialidases and estimated their activity and specificity towards SA residues. Methods: Several bacterial, viral and human sialidases were tested. We adopted bacterial sialidases from Salmonella and Actinomyces for expression on the cell surface by fusing catalytic domains with transmembrane domains. We also selected Influenza A/PuertoRico/8/34/H1N1 neuraminidase and human membrane sialidase ( hNeu3) genes. Lectin binding assay was used for estimation of a α (2,3)-sialylation level by fluorescent microscopy and FACS. Results: We compared sialidases from bacteria, Influenza virus and human. Sialidases from Salmonella and Influenza A neuraminidase effectively cleaved α (2-3)-SA receptors. Viral neuraminidase demonstrated a higher activity. Sialidases from Actinomyces and hNeu3 did not show any activity against α (2-3) SA under physiological conditions. Conclusion: Our results demonstrated that sialidases with different specificity and activity can be selected as genes providing antiviral defence. Combining chosen sialidases with different activity together with tissue-specific promoters would provide an optimal level of desialylation. Tissue specific expression of the sialidases could protect domestic birds from infection. |
url |
https://f1000research.com/articles/7-206/v2 |
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